ABSTRACT
Objective@#To investigate the status of nurses' humble leaders and nurses in seeking feedback, and to analyze the relationship between them.@*Methods@#From August to September 2018, 436 nurses from Beijing Luo he Hospital affiliated to Capital Medical University were selected as the research subjects. The questionnaires were surveyed using general data questionnaire, humble leadership scale and feedback seeking scale.@*Results@#The head nurse′s humble leadership scale was divided into (3.22±0.38) points, and the nurses' feedback seeking behavior was divided into (3.51±0.56) points. The head nurse′s humble leadership was positively correlated with the nurses' feedback seeking behavior (r=0.679, P<0.05).@*Conclusions@#The head nurse′s humble leadership can positively promote the nurse′s feedback seeking behavior. The head nurse should pay attention to the leadership qualities of the humble leader, so as to effectively improve the level of feedback seeking behavior.
ABSTRACT
Objective To investigate the status of nurses' humble leaders and nurses in seeking feedback, and to analyze the relationship between them. Methods From August to September 2018, 436 nurses from Beijing Luo he Hospital affiliated to Capital Medical University were selected as the research subjects. The questionnaires were surveyed using general data questionnaire, humble leadership scale and feedback seeking scale. Results The head nurse′s humble leadership scale was divided into (3.22 ± 0.38) points, and the nurses' feedback seeking behavior was divided into (3.51 ± 0.56) points. The head nurse′s humble leadership was positively correlated with the nurses'feedback seeking behavior (r=0.679, P<0.05). Conclusions The head nurse′s humble leadership can positively promote the nurse′s feedback seeking behavior. The head nurse should pay attention to the leadership qualities of the humble leader, so as to effectively improve the level of feedback seeking behavior.
ABSTRACT
Objective To establish antigen capture ELISA methed to detect Kaposi's sarcoma-as-sociated herpesvirus(KSHV)antigen,and to evaluate its feasibility for clinical application.Methods The BALB/c mice and New Zealand white rabbits were injected with purified recombinant KSHV gpK8.1 proteins to prepare the monoclonal antibody(McAb)and polyclonal antibody(PcAb)anti-gpK8.1,respectively.A new antigen capture ELISA method was established for KSHV antigen detection.The detection reproducibili-ty as well as the sensitivity and specificity of this new assay were determined by the optimization test,which antibody pairs were analyzed to choose the best coating antibody and detecting antibody.The 3 KSHV posi-tive patients sera and 257 patients sera from sexually transmitted disease,cancers or gynecological diseases were detected with this assay to evaluate its value for clinical application.Results When the McAb as coat-ing antibody at concentration of 5 μg/ml and PcAb as detecting antibody at concentration of 1.6μg/ml were selected,the highest P/N value could be obtained.The sensitive analysis of this test could detect recombi-nant KSHV gpK8.1 antigen of 31.28 ng/ml.Meanwhile,it is highly specific to detect KSHV antigen with-out cross reaction to Epstein-Barr vims(EBV),herpes simplex virus(HSV)-1 or HSV-2.All of three KSHV-positive sera and 4 sera from 257 clinical samples were positive with this new assay.which indicated that it could be used for capturing KSHV antigen.Conclusion A sensitive and specific McAb-based anti-gen capture ELISA method to detect KSHV antigen were established successfully.It is of great potential val-ue to develop reagent for KSHV clinical serologic dingnosis.
ABSTRACT
Objective To improve the success rate of CT examination on child care patients by summarizing the nursing experience.involved in the examination process. Methoods 500 patients were examined by CT,who had accepted relative nursing treatment during the whole checkup process. Results Imaging quality was good in 495 patients,poor in 5. Conclusion To acquire a good imaging quality and to secure child care safety,it is essential that the nursing experience be applied.