ABSTRACT
Objective:To investigate the optimal dose of Th1/Th2 immuno-enhancement effects of cultivated Artemisia rupestris L. crude polysaccharides (CARCP) on foot-and-mouth disease vaccine (FMDV) via the intramuscular route. Methods:ICR mice were intramuscularly immunized twice with different concentrations of CARCP mixed with FMDV at 2-week intervals. FMDV-specific antibodies, isotypes, and IgE in serum were detected by ELISA. Splenocyte proliferation was detected by MTT. T lymphocyte subsets and cytokines in the spleen were detected by flow cytometry. Clinical signs and local reactions at the injection site were monitored daily, and the body weight of mice was weighed after immunization.Results:The medium dose of CARCP could significantly improve FMDV-specific IgG, IgG 1, and IgG 2a antibody levels and the IgG 2a/IgG 1 ratio ( P<0.05) and lead to significant splenocyte proliferative responses ( P<0.01). The medium dose of CARCP could also significantly increase the level of CD3 +CD4 + and CD3 +CD8 + T cells as well as CD4 +/CD8 + ratio ( P<0.05), elicited the higher levels of IFN-γ in CD4 + T cells and CD8 + T cells ( P<0.05). No local adverse reactions at the injection site were observed after immunization. There was no significant difference in body weight or growth between each group( P>0.05). CARCP did not significantly induce an IgE response ( P>0.05). Conclusions:CARCP as an FMDV adjuvant promotes Th1/Th2 immune responses, especially in favor of the Th1 response, and has a certain safety profile. The best immune enhancement is achieved with CARCP at medium doses.
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Objective: To analyze the clinical application effect and satisfaction of a self designed multifunctional wire arranging plate in the nursing care of operation rooms. Methods: 108 patients who had received abdominal operation were divided into observation group (54 cases) and control group (54 cases) according to random method. And risk nursing was applied in both of two groups, while a multifunctional wire arranging plate was given additionally to the observation group. The psychological comfort level, quality of care and satisfaction between the two groups were compared. Results: The postoperative SAS and SDS scores of both groups were better than that of preoperative scores, and the postoperative SAS and SDS of observation group were significantly higher than that of control group (t=9.2126, t=13.3934, P<0.01). And the scores of basic nursing, aseptic condition, nursing record, goods management and safety management of the observation group were (96.23±3.24), (95.56±7.23), (93.36±6.32), (93.65±6.45) and (95.69±2.65), respectively. And all of them were significantly better than that of the control group (t=12.1185, t=13.8281, t=8.7427, t=11.0142, t=10.0133, P<0.01). The difference of total satisfaction rate of nursing between the observation group (94.44%) and control group (79.63%) was significant (x2=5.2523, P<0.05). Conclusion: The application of a multifunctional wire arranging plate for nursing care in operation rooms can effectively enhance the psychological comfort level of patients, and promote the quality improvement of nursing, and enhance nursing satisfaction of patients.
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of parthenolide on the proliferation of vascular smooth muscle cell(VSMC) and its mechanism.</p><p><b>METHOD</b>Vascular smooth muscle cell was cultured, the protein levels of c-fos, c-myc, p15, p16, p18, p19 were measured by Western blot method, cell cycle were examined with flow cytometry, and the DNA synthesis was determined by [3H]-TdR incorporation.</p><p><b>RESULT</b>Parthenolide inhibited protein levels of c-fos, c-myc in a time-dependent manner but didn't affect the protein levels of p15, p16, p18, p19. Flow cytometric DNA analysis revealed that parthenolide increased significantly G0/G1 phase of VSMC and decreased S phase of VSMC in a dose-dependent manner. Parthenolide inhibited [3H]-TdR incorporation in a dose dependent manner.</p><p><b>CONCLUSION</b>Parthenolide may inhibit proliferation of VSMC by inhibiting the expressions of c-fos, c-myc, but not the expressions of p15, p16, p18, p19.</p>