ABSTRACT
Objective: To explore the effect of 5-Aza-2′ -deoxycytidine (5-Aza-CdR) on ERα expression, proliferation and invasion of human breast cancer cell line MDA-MB-231. Methods: The breast cancer cell line MDA-MB-231 was treated with 5-Aza-CdR. The effect of 5-Aza-CdR on proliferation was evaluated by MTT assay and cell growth curve was drawn. The cell cycle was analyzed by flow cytometry. Immunohistochemistry and RT-PCR were used to determine the changes of ERα expression and matrix metalloproteinase-2 (MMP-2) expression in the MDA-MB-231 cell after 5-Aza-CdR treatment. The invasion ability of MDA-MB-231 cells was tested by Boyden chamber assay. Results: Compared with the control, the cell growth speed was significantly slowed down and the cell cycle was arrested at G0/G1 phase after 5-Aza-CdR treatment. The expression of ERα gene was partly restored in ERα-negative human breast cancer MDA-MB-231 cell after 5-Aza-CdR treatment. The expression of MMP-2 in MDA-MB-231 cells was down-regulated and the invasion ability of MDA-MB-231 cell was decreased after 5-Aza-CdR treatment. Conclusion: 5-Aza-CdR treatment effectively inhibited cell proliferation, induced cell cycle arrest, and decreased cell invasion ability of breast cancer MDA-MB-231 cells. The findings suggested that the partial restoration of ERα expression and the decrease in MMP-2 expression may be one of the mechanisms for the biological behaviour changes of MDA-MB-231 cells induced by 5-Aza-CdR.
ABSTRACT
AIM: To study the changes in anterior chamber angle after phacoemulsification and foldable IOL implantation with ultrasound biomicroscopy.METHODS: Small-incision phacoemulsification and foldable IOL implantation were performed in 50 eyes of 46 senior patients, and the changes of anterior chamber angle were determined quantitatively by using ultrasound biomicroscopy before and one month after the surgery.RESULTS: In all patients, the angle was widened significantly one month after the surgery (P＜0.01). The measurements of TIA500 ( trabecular-iris angle at 500μm from the scleral spur) , AOD250 (angle-opening distance at 250μm from the scleral spur) and AOD500 (angle-opening distance at 500μm from the scleral spur) increased significantly after the operation ( P＜ 0.01). The mean post/pre-operative TIA500ratio, AOD250 ratio and AOD500 ratio were 1.65 (1.12-4.91),1.81 (1.06-2.67) and 1.65 (1.01-2.76), respectively. A significant negative correlation existed between preoperative and postoperative data.CONCLUSION: Small-incision cataract surgery deepens the anterior chamber and widens anterior chamber angle significantly in senior patients. The narrower the preoperative angle, the higher ratio of post/preoperative ratio found.