Initial study on the discrimination of oral common Actinomycetes with metabonomics method / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The method of metabonomics based on 1H-nuclear magnetic resonance (1H-NMR) was preliminarily applied to discriminate the oral common Actinomycetes, Actinomyces naeslundii ATCC12104 and Actinomyces israelii ATCC12102.</p><p><b>METHODS</b>Solutions of Actinomyces naeslundii and Actinomyces israelii with same density were made and cultured respectively at BHI liquid culture medium. The concentration of bacteria was determined periodically, and then the growth curves were drawn. The culture solutions in stationary phase of the two bacteria were used to test with the 1H-NMR spectroscopy respectively. The data of 1H-NMR spectroscopy results were analyzed by principal components analysis (PCA).</p><p><b>RESULTS</b>The PCA showed the obvious clustering phenomena and the points of two groups data stayed differentially together by two clusters. Therefore, the NMR-based metabolomics profiles can discriminate the two different kinds of bacteria.</p><p><b>CONCLUSION</b>The analysis technology of metabonomics is expected to be applied to rapid identification of actinomycetes.</p>

Discrimination of common oral streptococcus with metabonomics method / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the feasibility of identifying oral streptococcus by comparing their metabolic profiling, and to find a convenient and rapid way to discriminate oral microorganisms.</p><p><b>METHODS</b>The pure cultivation of Streptococcus sanguis ATCC 10556 and Streptococcus sobrinus 6715 (reference strain) from solid culture were respectively inoculated in TPY liquid medium. Then the growth quantity was measured periodically by turbidimetry and the growth curves of the inoculated bacteria were completed. The culture solutions in the stationary phase of the two bacteria were centrifuged, and then tested with the 1H-nuclear magnetic resonance (1H-NMR) spectrometer respectively. The gained free induction decay (FID) data were all inputted into MestReC Soft and finally transformed into metabolic profiling. The metabolic profiles were integrated segmentingly and the results were inputted into SIMCA-P Soft for principal components analysis (PCA).</p><p><b>RESULTS</b>The PCA results showed the obvious clustering phenomena and the points of two group data differentially centralized in two clusters. Therefore, the NMR-based metabonomics profiles can discriminate the two different kinds of bacteria.</p><p><b>CONCLUSION</b>The metabonomics can be expected to be a kind of promising useful method in quick discrimination of oral streptococcus.</p>

Preliminary study on the discrimination of putative periodontal pathogens with a metabonomics method / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the feasibility of identifying oral pathogenic bacteria by comparing the metabolic profiling of putative periodontal pathogens and try to find a convenient and rapid way to discriminate oral microorganisms.</p><p><b>METHODS</b>Suspensions of Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum with same density were prepared and cultured respectively at liquid BHI medium. Then the growth quantity was measured periodically through turbidimetry and the growth curves of the inoculated bacteria were completed. The culture solutions of stable growth phase were sampled and characterized by 1H-nuclear magnetic resonance 1H-NMR). The data of 1H-NMR spectroscope results were analyzed by principal components analysis (PCA).</p><p><b>RESULTS</b>The PCA showed the obvious clustering phenomena and the points of three groups differentially centralized to three clusters. Therefore, the NMR-based metabonomics profiles could discriminate the three different kinds of bacteria.</p><p><b>CONCLUSION</b>The metabonomics is a potential classable method to identify the oral pathogenic bacteria.</p>

Initial study on the discrimination of oral microorganisms with a metabonomics method / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To establish the spectra of metabolites that coued be employed in identification of oral pathogenic bacteria, and try to find a convenient and rapid way to discriminate oral microorganisms.</p><p><b>METHODS</b>Suspensions of Streptococcus mutans ATCC 25175, Streptococcus sanguis ATCC 10556 and Lactobacillus acidophilus ATCC 4356 with same density were preparecd and cultured respectively at improved TPY liquid culture medium. The growth quantity were measured periodically by a turbidimeter. And the growth curves of the inoculated bacteria were completed. The culture solutions in stationary phase of the three bacteria were tested with 1H-nuclear magnetic resonance (1H-NMR) spectroscopy respectively. The data of 1H-NMR spectroscopy results were analyzed by principal components analysis (PCA).</p><p><b>RESULTS</b>The PCA showed the obvious clustering phenomena and the points of three group differentially centralized to three clusters. Therefore, the NMR-based metabonomics profiles could discriminate the three different kinds of bacteria.</p><p><b>CONCLUSION</b>The metabonomics is a promising new technology for developing to a rapid discrimination method of oral pathogenic bacteria.</p>