ABSTRACT
ObjectiveTo investigate the incidence and gene mutation spectrum of childhood thalassemia in Sanya city. MethodsThere were 938 children in our hospital screened by routine blood test, C-reactive protein test and hemoglobinelectro-phoresis. Based on the screening results in children with thalassemia and its subtypes, the alpha thalassaemia children were diag-nosed by gap - polymerase chain reaction, while the beta thalassaemia ones were diagnosed by polymerase chain reaction-reverse dot blot, and all the results were retrospectively analyzed.ResultsScreening positive rate of thalassemia anemia was 13.65% (128/938), genetic diagnosis positive rate was 11.41% (107/938). In 107 cases diagnosed with thalassemia by genetic test, 59 were alpha thalassaemia, and 46 were beta thalassaemia, and 2 cases were alpha thalassaemia combined with beta thalassaemia. In 59 cases of alpha thalassaemia, 31 had genotype of - SEA/alpha alpha , 13 cases with genotype alpha 4.2 / alpha alpha, and 6 were of HbH disease. In the 46 cases of beta thalassaemia children, six gene locus mutations were found : CD41-42 (CTTT) in 21 cases, TATAbox - 28 (A - G) in 13 cases, CDs14/15 (G) in 5 cases, IVS - Int 654 (C - > T) in 4 cases, CD17 (A - > T) in 2 cases, and 27-28 (C) in 1 case.ConclusionHigh prevalence of thalassemia in children from Sanya city warrants genetic counseling, premarital check-up and prenatal diagnosis.
ABSTRACT
OBJECTIVE:To study the feasibility of the quality standard of Potassium sodium pehydroandroandrographolide succinate for injection. METHODS:The contents of dehydroandyograpolide succinate in Potassium sodium dehydroandrograpolide succinate for injection from 13 manufacturers were determined by HPLC and UV spectometry.The related substances of all the samples were determined by HPLC and TLC,respectively. The feasibility of different assaying methods was compared. RESULTS:Determined by HPLC assay,the principal constituent and the degradation products could be separated. There were significant differences between different assaying methods in the determination results of the related substances of samples; TLC assay was inferior to HPLC in respect of the limit of quantitation and accuracy. CONCLUSION:The quality in a few enterprises remains to be improved and the quality standard of Potassium sodium pehydroandroandrographolide succinate for injection remains to be further improved.