ABSTRACT
OBJECTIVE To investigate the Ureaplasma urealyticum infection in patients with urogenital infections and the distribution of its serotypes and genotypes.METHODS The clinical samples were firstly screened with B-Merieux mycoplasma ID2 culture and identification kit,then the positive cultural samples were subtyped by PCR method.RESULTS The positive rate of U.urealyticum with cultural kit was 37.43%(670/1790).The 392 positive cultural samples were subtyped with PCR,the positive rate of U.parvum was 71.94% and the positive rate of U.urealyticum was 29.85%.The serotypes of 264 U.parvum positive samples were identified with PCR,there were 20 samples with serovar 1(7.58%),139 samples with serovar 3 or 14(52.65%),and 51 samples with serovar 6(19.32%).Ninety nine positive samples with U.urealyticum were genotyped,the results showed that the rate of genotype 3(serovar 7 or 11) was lower than genotypes 1 and 2.CONCLUSIONS PCR identification and subtyping of Ureaplasma in patients with urogenital infections showed that the major epidemic pathogen of Ureaplasma in east of Guangdong Province is U.urealyticum serovar 3 and serovar 6,and the distribution of different serotypes or genotypes of Ureaplasma in this area is specific.