ABSTRACT
Objective:To establish the quality standard for Lixue pills. Methods:TLC was used to identify the active ingredients, and HPLC was used to determine the content of hesperidin in the preparation. The analysis was performed on a WondaSil C18 column (250 mm ×4.6 mm,5 μm) with the mobile phase of acetonitrile-water-phosphoric acid(20:80:0.1)at a flow rate of 1.0 ml·min-1. The column temperature was maintained at 40℃. The detection wavelength was set at 284 nm. Results:The specific spots of the active ingredients were presented clearly by TLC without any interference from the negative control. The linearity of hesperidin was good with-in the range of 16. 560-82. 800 μg·ml-1(r=0. 999 8), and the average recovery was 98. 8%(RSD=1. 1%, n=6). Conclusion:The method is accurate and reliable, which can be used for the quality control of Lixue pills.
ABSTRACT
OBJECTIVE:To evaluate the environmental quality of planting bases of Artemisia argyi in Qichun county of Hubei province so as to provide scientific foundation for the construction of the planting bases of A. argyi which conforms to the require-ments of Good Agriculturing Practice (GAP). METHODS:By reference to national Ambient Air Quality Standards (GB 3095-1996),Water Quality Standards for Farm Irrigation(GB 5084-2005)and Soil Environment Quality Standards(GB 15618-1995), the ambient air,irrigation water and soil quality at 3 major producing areas of A. argyi,including Hongmenlou,Zhulinhu and Tongxin,were tested respectively. RESULTS:At the 3 major producing areas,the ambient air(including sulfur dioxide,nitrogen dioxide,total suspended particulates,fluoride,etc.)was up to primary standards in Ambient Air Quality Standards,irrigation wa-ter quality(including pH,chloride,sulfide,total arsenic,total mercury,lead,cadmium,fecal coligroup count,number of ascaris eggs,etc.)met the basic dry farming requirements in Water Quality Standards for Farm Irrigation,and soil quality(including pH, cadmium,mercury,lead,arsenic,chromium,copper,DDT,hexachlorocyclohexane,etc.) reached secondary standards in Soil Environment Quality Standards. CONCLUSIONS:The aforesaid results show that the planting bases meet GAP requirements in am-bient air,irrigation water and soil quality and are applicable producing areas of medicinal material A. argyi.
ABSTRACT
OBJECTIVE:To compare the contents of eucalyptol in Artemisia argyi of 5 different planting densities. METH-ODS:Gas chromatography method was performed on the column of HP-5 capillary column,and cyclohexanone was selected as in-ternal standard,the carrier gas was nitrogen at a flow rate of 0.6 ml/min,the volume temperature was 240℃,the detector tempera-ture was 250 ℃,the split ratio was 10∶1,and the injection volume was 1 μl. RESULTS:The linear range of eucalyptol was 0.420 4-1.261 2 mg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 0.92%;average recovery was 99.6%(RSD=1.4%,n=6). CONCLUSIONS:The method is simple,rapid,accurate,reliable and reproducible,and can be used for the content determination of eucalyptol in A. argyi. The optimal planting density of A. argyi was spaced 30 cm×40 cm.