ABSTRACT
Objective To investigate gene polymorphism of Kell blood group in different Zhuang population from Guangxi region .Methods The genotypes of Kell blood group of 1025 non‐related individuals in different areas of Guangxi Zhuang popula‐tion were analyzed by PCR‐SSP .Results The Kell antigen in all individuals was homozygous ,the gene frequency of K and Jsa was 0 ,while that of k and Jsb was 1 .000 .Conclusion The distribution characteristic of Kell blood group in Guangxi Zhuang population was monomorphism ,which was similar to other Chinese population reported by literatures .
ABSTRACT
Objective To investigate the distribution situation of ABO and Rh blood groups among Zhuang population in Nanning area to guide the scientific voluntary blood donor recruitment and rational storage of blood in this area.Methods 2 052 blood samples from Zhuang population in Nanning area were performed the identification of ABO and Rh blood groups.Whether the gene distributions conforming to the Hardy-Weinberg equilibrium principle was assessed by using the Chi-square test.The data was compared with the distribution characteristics in other areas or other minorities.Results The distribution characteristics of ABO blood groups in Nanning area were O > B> A > AB.Blood group O was highest (46.78%)and blood group AB was lowest (4.34%).The gene frequencies of A,B and O were 0.131 9,0.181 5 and 0.686 6,respectively.The blood groups distribution con-formed to the Hardy-Weinberg equilibrium principle;the RhD positive proportion(RhD+ )in Zhuang population was 99.90%,while the RhD negative (RhD- )proportion was 0.10%,which was lower than that in Han population.No phenotypes of ccdE,CcdE, CCdee,CCdE,CCDEE and ccDee were observed in this study.Conclusion The distribution of the ABO and Rh blood groups among Zhuang population in Nanning still maintains their own exclusive characteristics.The extremely low proportion of RhD- will bring about serious difficulties to the patients with long term blood transfusion.
ABSTRACT
Aim To investigate the inhibitory effect of peptide from Agkistrodon Acutus snake venom(fraction K)on the growth of human ovarian carcimona A2780 cell line and its mechanism.Methods The A2780 cells were treated with fraction K at different concentrations.The proliferation of A2780 cells was assayed with MTT colorimetric method.The histologi-cal and ultras-tructural changes of cell were scored using light and electron microseopy.The apoptosis of A2780 cells induced by fraction K was studied by fluorescent staining.Crystal violet staining and MTT assay were employed to determine the effect of fraction K on A2780 cells that they had been adhered to fibronectin.Result Proliferation of A2780 cells was inhibited significantly by fraction K in a dose-and time-dependant manner.Under electron-microseop and fluorescence microscope,some A2780 cells underwent a typical apoptosis and morphology changes after 24 hours incubation with fraction K.Fraction K could inhibit the adhesion of A2780 cells to fibronectin in a dose-dependent manner.Conclusion fraction K can inhibit proliferation of A2780 cells,which may be related to the mechanism of inducing cellular apoptosis and cellular adhesion.
ABSTRACT
Objective To evaluate the efficacy and safety of magnesium sulfate in the treatment of acute stroke.Methods The patients were randomly divided into treatment group and control group.Each group was treated with citicoline(CDPC)as a primary treatment.Magnesium sulfate was added to the treatment group for fifteen days.Results The therapeutic effect of the treatment group was significantly better than that of control group,with mild adverse effects.Conclusion Magnesium sulfate is effective and safe in the treatment of acute stroke.