ABSTRACT
Objective: To investigate the effect of berberine on angiogenesis and signal transduction pathway of hypoxia-inducible growth factor-1alpha [HIF-1 alpha] / vascular endothelial growth factor [VEGF] in rats with cerebral ischemia-reperfusion injury. Study Design: An experimental study. Place and Duration of Study: Luoyang Central Hospital Affiliated with Zhengzhou University, China, from 2016 to 2017
Methodology: Forty-five rats were randomly divided into control group, model group and berberine group, 15 rats in each group. The model of rat with cerebral ischemia-reperfusion injury was duplicated by suture method. Neurological score was evaluated before, after operation, and 7 days after administration. Microvessel density [MVD] of cerebral ischemiareperfusion cortex was detected by immunohistochemistry. HIF-1 alpha mRNA and VEGF mRNA of cerebral ischemia reperfusion cortex were detected by RT-PCR. HIF-1 alpha, and VEGF protein expression of cerebral ischemia-reperfusion cortex was detected by Western Blotting
Results: After operation and 7 days of administration, the neurological scores of the control group, model group, and berberine group were different [all p<0.001]. After 7 days of administration, neurological score of berberine group was lower than that of model group [p<0.001], MVD, HIF-1 alpha mRNA and VEGF mRNA expression. HIF-1 alpha and VEGF protein expression levels were lower in model group than berberine group [all p<0.001]
Conclusion: Berberine can promote angiogenesis in the rat with cerebral ischemia-reperfusion injury. Its mechanism may be activation of HIF-1 alpha / VEGF signal transduction pathway
ABSTRACT
Objective: To investigate the effect of berberine on angiogenesis and signal transduction pathway of hypoxia-inducible growth factor-1Alpha [HIF-1Alpha] / vascular endothelial growth factor [VEGF] in rats with cerebral ischemia-reperfusion injury
Study Design: An experimental study
Place and Duration of Study: Luoyang Central Hospital Affiliated with Zhengzhou University, China, from 2016 to 2017
Methodology: Forty-five rats were randomly divided into control group, model group and berberine group, 15 rats in each group. The model of rat with cerebral ischemia-reperfusion injury was duplicated by suture method. Neurological score was evaluated before, after operation, and 7 days after administration. Microvessel density [MVD] of cerebral ischemiareperfusion cortex was detected by immunohistochemistry. HIF-1Alpha mRNA and VEGF mRNA of cerebral ischemiareperfusion cortex were detected by RT-PCR. HIF-1Ñ, and VEGF protein expression of cerebral ischemia-reperfusion cortex was detected by Western Blotting
Results: After operation and 7 days of administration, the neurological scores of the control group, model group, and berberine group were different [all p<0.001]. After 7 days of administration, neurological score of berberine group was lower than that of model group [p <0.001], MVD, HIF-1Alpha mRNA and VEGF mRNA expression. HIF-1Alpha and VEGF protein expression levels were lower in model group than berberine group [all p <0.001]
Conclusion: Berberine can promote angiogenesis in the rat with cerebral ischemia-reperfusion injury. Its mechanism may be activation of HIF-1Alpha / VEGF signal transduction pathway
ABSTRACT
BACKGROUND:There is a close relationship between epilepsy and apoptosis. The appearance of epilepsy can lead to the loss of neurons in the hippocampus, triggering a series of programmed cel death. OBJECTIVE:To investigate the effect of bone marrow stromal stem cel transplantation on apoptosis in epilepsy. METHODS:After modeled to be of epilepsy 45, Sprague-Dawley model rats were randomly divided into three groups, fol owed by given no intervention (moldel group), normal saline (normal saline group) or bone marrow stromal stem cel transplantation (transplantation group). At 1, 2 and 4 weeks after modeling, the number of Bax-positive cel s, Bcl-2-positive cel s and Bax/Bcl-2 were detected by immunohistochemistry. RESULTS AND CONCLUSION:The number of Bax-positive cel s, Bcl-2-positive cel s and Bax/Bcl-2 presented no obvious changes in the normal saline group at different time points. However, the number of Bax-positive cel s and Bax/Bcl-2 in the transplantation group was significantly decreased, while the number of Bcl-2-positive cel s significantly increased compared with the other two groups at 1, 2 and 4 weeks after modeling (P<0.05). Moreover, the above indicators varied significantly in the transplantation group at different time points after modeling (P<0.05). These results show that bone marrow stromal stem cel transplantation can affect the apoptosis and effectively reduce the apoptosis in rats with epilepsy by up-regulating the number of Bax-positive cel s and down-regulating the number of Bcl-2-positive cel s.
ABSTRACT
Objective To compare the serum microelement changes between patients aged ≥80years and adults aged ＜ 60 years,and to compare the levels of serum microelement,total protein,albumin,hemoglobin between the long term in-bed and non in-bed oldest patients.Methods Beijing Bohui BH5100 type atomic absorption spectrometer was used to determine serum microelement.Total protein,albumin,hemoglobin were also determined.Results The amounts of ferrum,zinc,calcium,copper,magnesium were (7.21 ± 1.21) mmol/L,(93.29 ± 10.96)mmol/L,(1.63 ± 0.21)mmol/L,(17.31±3.61)mmol/L,(1.49±0.20)mmol/L in people aged over 80 years,(8.91±1.25)mmol/L,(121.85±11.24)mmol/L,(1.51±0.23)mmol/L,(15.89±3.87)mmol/L,(1.51±0.21)mmol/L in people aged less than 60 years,respectively.The ratios of Ferrum deficiency were 42.9 ％,zinc deficiency 14.3％,calcium deficiency 21.4％,copper deficiency 14.3％ in oldest-old,whereas were 9.1％,13.6 ％,31.8％,27.3％ in people aged less than 60 years.The amount of ferrum was significantly lower in people aged over 80 years than in people aged less than 60 years (P＜0.01).Proportion of ferrum deficiency was higher in patients aged over 80 years (P＜0.01).The amount of calcium and copper was markedly lower in people aged less than 60 years than in people aged over 80 years (P＜0.01).The proportions of calcium,copper deficiency were higher in people aged less than 60 years (P＜0.01).The amounts of ferrum,zinc,total protein,albumin,hemoglobin of the patients aged over 80 years in long-term in-bed group were significant decreased as compared with those of people in non long-term in-bed group [(6.44±0.89)mmol/L vs.(8.16±1.16)mmol/L,(84.48±8.98)mmol/L vs.(103.62±10.31)mmol/L,(62.8±3.9)g/L vs.(66.3±1.7)g/L,(33.7±2.6)g/L vs.(36.7±l.8)g/L,(109.0±12.5)g/L vs.(132.0±5.2)g/L,all P＜0.01].The amount of calcium of people in none long-term group was much lower [(1.57±0.23)mmol/L vs.(1.71±0.19)mmol/L,P＜0.01].Ferrum,zinc,calcium and copper deficiencies were 60.9％,18.8％,13.0％,13.0％ in long-term in-bed group,21.1％,8.8％,31.6％,15.8％ in non long-term in-bed group.Proportion of ferrum deficiency was higher in long term in-bed group than non long-term in-bed group (P＜0.01).Proportion of calcium deficiency was higher in non long-term in-bed group than long-term in bed group (P＜0.01).There was no significant difference in the amount of copper between people in long term in bed group and non long-term in-bed group (P＞0.05).There was no absence of magnesium in both two groups.Conclusions There is more absence of ferrum,zinc,total protein,albumin,hemoglobin in the patients aged 80 years and over in long-term in-bed.There is more calcium and copper absence of people aged less than 60 years.So it is necessary to supply high ferrum food and nutrients in order to get well sooner,especially to patients aged over 80 years.