ABSTRACT
AIM: To elucidate the cellular source of adrenomedullin (AM) in myocardial tissue, the effects of 5 substances on secretion of AM from cultured rat fibroblasts and myocytes were examined in this study. METHODS: Myocardial fibroblast and myocyte isolation and culture; AM was measured by radioimmunoassay; AM receptors were assayed by radioactive analysis method. RESULTS:Tumor necrosis factor ? (TNF?), lipopolysaccharide (LPS) and basic fibroblast growth factor (bFGF) all could stimulate AM synthesis and secretion in myocardial Fb and myocyte , and the amount of AM secreted by Fb was greater than that by myocytes, whereas transforming growth factor-?(TGF?),and interferon-?(IFN?) suppressed in it in myocardial Fb and myocytes. Myocardial Fb has two kinds of AM binding sites, but myocyte has only one high affinity- binding site.CONCLUSIOIN:Myocardial fibroblasts could synthesize and secrete AM, and there are AM receptors in both myocardial fibroblasts and myocytes.
ABSTRACT
Adrenomedullin is a vasodilative peptide found in 1993. It has been proved that proadrenomedullin can be divided into 4 peptides by endoenzyme. Proadrenomedullin N terminal 20 peptide (PAMP) is one of them. PAMP has many functions on cardiovascular physiology. But so far the relationship between PAMP and cardiovascular diseases has not been found. In this study its plasma levels in the patients with essential hypertension(EH) and heart failure(HF) were investigated with its pathophysiological effect discussed. The plasma levels of PAMP, endothilin(ET), Angiotension II(AT II) were measured with RIA, and norepinephrine(NE), epinephrine(E) were measured with HPLC. the plasma level of PAMP in the control group was 18 42?2 33ng/L, and was 1 62 times higher in EH group than in the control group, and was 1 53 times higher in HF group as compared with the control group respectively ( P
ABSTRACT
The activities of serum PK of 51 cases with AMI were determined. It was raised in all of the 43 cases with AMI within 24h after admission. Serum PK was specific for the early diagnosis of AMI