ABSTRACT
Objective To explore the role of protein kinase(PKC)in the nosogenesis of hypoxic-ischemic encephalopathy(HIE).Methods Twenty-four seven-day-old SD rats were divided randomly into normal group (N),model control group(C)and HIE group(HIE).The biochemistry techniques of SDS-PAGE and western bolt were used to examine the level of PKC membrane translocation of cortex and hippocampi.Results The level of PKC membrane translocation of cortex and hippocampi in N group was 0.28±0.02,0.29±0.02 respectively,and 0.29±0.02,0.30±0.02 in C group,and 0.38±0.04,0.40±0.06 in group HIE.The level of PKC membrane translocation of cortex and hippocampi was higher in HIE group as compared with that in N,C group(P＜0.01),but there was no different between N group and C group(P＞0.05).Conclusion PKC are activated in HIE,which indicates that PKC may involve in the pathophysiologic mechanism of HIE.
ABSTRACT
Objective To explore the effects of neuronal apoptosis in the hippocampal CA 1 region by the selective nitric oxide synthase(NOS) inhibitor, 7 nitroindazole (7 NI).Methods 72 postnatal 7 day old Wistar rats were randomly divided into 3 groups.(1)Sham operation group:12 rats were cut in their neck centre without ligature in common carotid artery;(2)Obersevertion group(7 NI group):the abdominal cavity of 30 rats were injected intraperitoneally into 7 NI (25 mg/kg) after anoxic ischemic damaged models were made;(3)The control group(the normal saline):30 rats were injected intraperitoneally into the normal saline the same volume after HIBD.The sham operative group was later than the operative one.The rats in the observation group and the control one by killed later,they were cut heads and brains in 48h.and were detected by HE and TUNEL stainning,the numbers of cerebral apoptosis were detected under the lightmicroscope.Results Typal apoptotic cells in the hippocampal CA 1 region in the control group were detected by HE stainning. They showed cell pyknosis,cytoplasm deep stain,nucleus concentration,fragment,karyoplasm unclear,apoptosis corpuscle was formed;in 7 NI group the above apoptosis decresed significantly;There was significant difference between Group 2 and Group 3(TUNEL positive apoptotic cells in Group 2)( P