ABSTRACT
AIM To investigate the influences of Astragalus and Hurido effective components on apoptosis of rat glomerular mesangial cells.METHODS The proliferation of rat mesangial cells was induced by LPS and then intervened with Astragalus (astragalus polysaccharide,astragaloside Ⅳ,calycosin) and Hurido (hirudin).The cells were collected after 24 h.4',6-Diamidino-2-phenylindole (DAPI) staining was used to observe cell nuclear changes.Quantitative and qualitative expressions of Bcl-2 were detected by streptavidin-biotin complex (SABC).RESULTS Astragalus and Hurido effective components could induce the apoptosis of rat glomerular mesangial cells,and decrease the expression of Bcl-2.CONCLUSION Astragalus and Hurido effective components can induce the mesangial cell apoptosis by down-regulating Bcl-2 expression,which can be used for the treatment of mesangial proliferative glomerulonephritis.
ABSTRACT
This paper reviewed the clinical research progress of the treatment of children's adenoid hypertrophy in recent years from the aspects of Chinese medicine treatment and combined traditional Chinese and western medicine treatment. It is believed thatTraditional Chinese medicine treatment for the children with adenoid hypertrophyhas showed better curative effect and less side effects, which can reduce the operation rates. It's easy for parents and children to accept and can be widely promoted in clinical.
ABSTRACT
Objective] To explore both the correlation of matrix metal oproteinase and the imbalance of its tissue inhibitors-MMP-2 and TIMP-2 with glomerulosclerosis and its research progress in traditional Chinese medicine. [Methods] We generalized the research progress in traditional Chinese medicine from such aspects as the biological properties of MMP-2 and TMIP-2 and their mechanisms, their relationship with mesangial proliferative glomerulonephritis and effects of traditional Chinese medicine on their expression by searching the relevant literatures. [Results] Expressions of MMP-2 and TIMP-2 vary in different pathological stages(hyperplasia and sclerosis stage here) of glomerulonephritis, and traditional Chinese herbs could adjust their expressions in different stages. Traditional Chinese herbs can inhibit the development of glomerulosclerosis. [Conclusion] Applying traditional Chinese medicine in the treatment of mesangialproliferative glomerulonephritis could provide the base for further development of TCM as wel as a new choice for treating mesangialproliferative glomerulonephritis.
ABSTRACT
For example of children virus pneumonia, we put forward that clinical research should be carried out by review article analysis, experts’opinions integrated and the requests of evidence-based medicine. On the base of systemic evaluation of the sensitivity, reliability, validity and relation among them of all indicators for effect evaluation on virus pneumonia, we will establish the effect evaluation system of TCM in the treatment of virus pneumonia including disease and syndrome effect evaluation, complication and combining drug,using rate evaluation, safety evaluation and health economics evaluation.
ABSTRACT
Objective:To investigate effect of the serum contained Radix Astragali, Hirudo, Hirudin and the compounding of these two herbs respectively on the growth cycle and apoptosis of rats' glomerular mesangial cells(GMCs)and observe the morphology of apoptosis. Methods: The growth cycle and apoptosis of GMCs was measured by flow cytometer. Meanwhile morphous of apoptosis was visualized by Wright's staining and observed by microscope. Results: The serum contained Radix Astragali, Hirudo, Hirudin and the compounding respectively can keep most GMCs in the stage of G0/G1,and it had a significant deviation compared with the control(P
ABSTRACT
Objective To study the effect of the serum contained Radix Astragali, Hirudo or their compound on the growth cycle and apoptosis of rat mesangial cells (MC).Methods The serum contained the extract of high-concentration of Hirudo, the serum contained the extract of high-concentration of Radix Astragali, and the serum contained the extract of high-concentration of Hirudo and the extract of low-concentration of Radix Astragali were prepared. The cultured MC were measured by flow cytometer to observe the changes in their growth cycle and apoptosis. The morphological changes of apoptotic cells were observed.Results The medicinal serums can keep the most MC in the stage of G0/G1, and the significant difference was existing in comparison with that of the control group (P