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Aim To investigate the effect of curcumin extract on melanin production and melanosome transport, and to explore the possible mechanism of the curcumin extract on microenvironment. Methods (1) B16F10 and HaCaT cells were cultured with different concentrations of curcumin. The proliferation ability was detected by MTT method. Melanin synthesis and tyrosinase activity in B16F10 cells were detected by NaOH pyrolysis method and Oxidation dopamine response in vitro. The expression levels of key proteins were detected by Western blot. (2) B16F10 cells were cultured with different concentrations of ISG15 protein. NaOH pyrolysis method and Oxidation dopamine response in vitro were used to detect melanin synthesis and tyrosinase activity in B16F10 cells. Results Curcumin could directly inhibited tyrosinase activity and melanin production, and inhibit melanocyte migration within a certain concentration range. ISG15 protein could enhance the melanin production, tyrosinase activity. Curcumin could reduce the expression of the ISG15 in HaCaT cell, change the microenvironment of melanocyte, and indirectly inhibit melanin synthesis through ISG15. Conclusions In addition to directly inhibiting melanin synthesis, curcumin can also play an indirect role in inhibiting melanin synthesis by inhibiting the expression of ISG15 protein and altering the microenvironment of melanocytes.
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OBJECTIVES@#To study the changes in the distribution and drug resistance profiles of pathogens causing bloodstream infection after chemotherapy in children with acute lymphoblastic leukemia.@*METHODS@#The medical data were collected from the children with acute lymphoblastic leukemia who were admitted to the First Affiliated Hospital of Zhengzhou University between January 2015 and December 2020 and developed bloodstream infection after chemotherapy. The samples were divided into the first three years group and the next three years group according to the time of testing to investigate the differences in the distribution and drug resistance profiles of pathogens as time.@*RESULTS@#A total of 235 strains of pathogens were isolated, among which there were 159 Gram-negative strains (67.7%; mainly Escherichia coli and Klebsiella pneumoniae), 61 Gram-positive strains (26.0%; mainly Staphylococcus epidermidis), and 15 strains of fungi (6.4%; mainly Candida albicans). There were no significant differences between the first three years group and the next three years group in the detection rate of Gram-negative bacteria (68.8% vs 66.9%, P>0.05) or Gram-positive bacteria (29.2% vs 23.7%, P>0.05). Compared with the first three years group, the next three years group had significant increases in the detection rate of Streptococcus mitis (5.8% vs 0.0%, P<0.05) and fungi (9.4% vs 2.1%, P<0.05). There was no significant difference in the drug resistance rate of Gram-negative or Gram-positive bacteria between the two groups (P>0.05).@*CONCLUSIONS@#Enterobacteriaceae bacteria are the main pathogens of bloodstream infection after chemotherapy in children with acute lymphoblastic leukemia, while the detection rates of Streptococcus mitis and fungi tend to increase as time, which needs to be taken seriously in clinical practice.
Subject(s)
Child , Humans , Anti-Bacterial Agents/therapeutic use , Bacteremia/drug therapy , Drug Resistance, Bacterial , Gram-Negative Bacteria , Methicillin-Resistant Staphylococcus aureus , Microbial Sensitivity Tests , Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy , Retrospective Studies , Sepsis/drug therapyABSTRACT
Objective: To study the relative molecular mass and composition of Ligustrum lucidum polysaccharide purified by dialysis, and provide the theories for the relationship between the biological activity and the internal composition. Methods: In this paper, L. lucidum as an object was studied. Polysaccharide was isolated by water extraction and ethyl alcohol precipitation and purified by Sevage method, hydrogen peroxide and dialysis. After the acidolysis of trifluoroacetic acid, the molecular weight was measured by GPC, and the composition of polysaccharide was analyzed by HPLC-RID. Results: The Mw of polysaccharide was 10 721, and the Mn of polysaccharide was 10 673. L. lucidum polysaccharide consisted of glucose, rhamnose, and arabinose, the molar ratio of these monosaccharide was 9.148.105.18. Conclusion: The purified polysaccharide composition is more homogeneous, and the monosaccharides of polysaccharides was easily analyzed by HPLC-RID without column derivatization.
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Objective To understand the natural population dynamics and spatial distribution of Tyrophagus putrescentiae in storage flour,so as to provide an evidence for its prevention and control. Methods The samples from five sampling points in Wuhu City were collected monthly from January to December,2013,and examined and counted for T. putrescentiae. The disper-sion pattern target,Iwao's m*--x regression analysis and Taylor's lgS2-lg-x regression analysis were used for analyzing the spa-tial distribution pattern of T. putrescentiae in the storage flour. Results The peaks of population dynamics of T. putrescentiae were discovered in July and September. The indexes of dispersion were as follows:I>0,CA>0,m*/-x >1;and the linear re-gression equation of Iwao:m*=3.7403+1.0175-x (r=0.9958)and Taylor:lgS2=0.5004+1.1349 lg-x (r=0.8328) showed that the spatial distribution pattern of T. putrescentiae in the storage flour was assembled. Conclusion The peak of pop-ulation dynamics of T. putrescentiae in the storage flour in Wuhu City is a double peak type,and the spatial distribution pattern of T. putrescentiae is assembled.
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Objective To investigate the pollution status of Gohieria fusca in the air conditioner-filters of different places in Wuhu City. Methods The dust samples were collected from the filters of air-conditioners in dining rooms,shopping malls,ho-tels and households between June and September,2013,and G. fusca was detected in the dust samples. Results There were 430 dust samples collected and 98 were G. fusca positive with the breeding rate of 22.79%. The difference of breeding rates of G. fusca were statistically significant among the different places(χ2=18.294,P<0.05). Among 510.5 g dust samples in total, 783 G. fusca mites were detected with an average breeding density of 1.53 mite/g. Conclusion G. fusca breeds in the dust of air-conditioner filters in Wuhu City gravely.
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Objective To investigate the breeding of Carpoglyphus lactis in the storage Arillus longan,so as to provide the evidence for preventing the harm of C. lactis to traditional Chinese medicine. Methods Chinese herbal medicine warehouses were chosen as survey sites according to the breeding habits of mites,and the A. longan samples were stored more than 6 months. The mites were isolated and identified under a microscope. Result The C. lactis breeding rate was 20.0％(4/20)and the breeding density was 184.95 per sample. The constitute rates of adult,larva,dormancy body and egg were 58.39％, 30.41％,0.06％,and 11.14％respectively. Conclusion The breeding density of C. lactis is high in the stored A. longan,so the control and prevention of human intestinal acariasis should be strengthened.
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<p><b>OBJECTIVE</b>This study was to explore the expression of CD71, as a proliferation indicator, on cell proliferaration in hematologic malignancy and its correlation with Ki-67, so as to assess the feasibility of CD71 instead of Ki-67 for assaying cell proliferation by flow cytometry (FCM).</p><p><b>METHODS</b>(1) Compared with mature B lymphoctyes during stationary phase in peripheral blood from healthy people, the cell cycle and the expression of CD71 and Ki-67 of cell lines from patients with leukemia and lymphoma were examined, the correlation among CD71, S-phase cell fraction (SPF) and Ki-67 were analyzed; (2) Compared with mature B lymphoctyes in bone marrow from non-hematologic disease patients, the expression and correlation of CD71 and Ki-67 of all kinds of leukemic cells and myeloma cells from bone marrow were analyzed by using Ki-67/CD71/CD45/CD123, Ki-67/CD71/CD45/CD20 or Ki-67/CD71/CD45/CD138.</p><p><b>RESULTS</b>(1) in respect to the expression rate of CD71 on tumor cell lines, the expression rate of CD71 on HL-60 cells was (99.77 ± 0.064)%, the expression rate of CD71 on NB4 cells was (99.23 ± 0.12)%, the expression rate on THP-1 cells was (98.90 ± 0.30)% and the expression rate on K562 cells was (97.03 ± 0.15)% in myelogenous leukemia cell lines, the expression rate of CD71 on Raji cells was (99.35 ± 0.21)% and the expression rate on Mino cell was (96.95 ± 0.42)% in lymphoma cell lines, which were also obviously higher than that on cells of the control group (P < 0.05); (2) in respect to the expression rate of CD71 on tumor cells in bone marrow, the expression rate of CD71 on poorly differentiated AML(M1 and M2) cells was (51.50 ± 19.31)%, the expression rate of CD71 on acute promyelocytic leukemia (AML-M3) cells was (35.71 ± 14.02) %, the expression rate of CD71 on acute monocytic leukemia (AML-M5) cells was (30.54 ± 14.38)%, the expression rate of CD71 on acute T lymphoblastic leukemia cells was (68.40 ± 20.83)%, the expression rate of CD71 on acute B lymphoblastic leukemia was (39.67 ± 18.27)%, the expression rate of CD71 on multiple myeloma (MM) cells was (55.49 ± 18.15%), the expression rate of CD71 on chronic lymphocytic leukemia(CLL) was (1.32 ± 0.33%), which were also higher than that on cells in the control group(P < 0.05) except for CLL cells (P > 0.05); (3) CD71 had a positive linear corrlation with SPF in cell lines (r = 0.914, P < 0.05), and also had a positive linear corrlation with Ki-67 in cell lines and carcinoma cells from bone marrow (r = 0.894,r = 0.904, P < 0.05).</p><p><b>CONCLUSION</b>The CD71 can take the place of Ki-67 as an indicator of cell proliferation activity of hematologic malignancies and the determination CD71 by FCM is simpler and better than that of Ki-67 in respest of methodology.</p>
Subject(s)
Humans , Antigens, CD , Cell Division , Cell Proliferation , Flow Cytometry , Hematologic Neoplasms , Ki-67 Antigen , Receptors, TransferrinABSTRACT
To analyze the clinical results of orbicularis muscle shortening surgery and eyelid skin orbicularis muscle resection for senile entropion. ●METHODS: From January 01, 2006 to December 31, 2012, senile patients with lower eyelid were divided into two groups according to different surgical methods. Group A with the orbicularis muscle shortening improved operation in 20 cases (38 eyes), group B used the eyelid skin orbicularis muscle resection in 21 cases (36 eyes). The effects of surgery were followed-up postoperatively. ●RESULTS: Patients were followed up for 1 - 2a, 20 patients ( 38 eyes ) in group A were cured without recurrence; 7 eyes recurred in group B, the recurrence rate was 19%, the recurrence rate after surgery both groups were statistically significant (P ●CONCLUSlON: The effect of orbicularis muscle shortening improved operation for senile entropion is good and stable.