Oral Testosterone Undecanoate Capsules combined with Qilin Pills for late-onset hypogonadism in males / 中华男科学杂志

Objective@#To investigate the clinical effects of oral Testosterone Undecanoate Capsules (TUC) combined with Qilin Pills (QLP) on late-onset hypogonadism (LOH) in men.@*METHODS@#Sixty-three LOH patients meeting the inclusion criteria were randomly divided into a control group (aged ［48.4 ± 6.2］ yr, n = 32) and an experimental group (aged ［47.2 ± 5.6］ yr, n = 31) to be treated with oral TUC (80 mg, qd) and TUC + QLP (6g, tid), respectively, both for 3 months. Comparisons were made between the two groups of patients in the IIEF-5 scores, total testosterone (TT) levels, and scores in the Aging Males' Symptoms (AMS) scale before and after treatment.@*RESULTS@#After treatment, the patients of the experimental group, as compared with the controls, showed a significantly increased IIEF-5 score (21.7 ± 5.8 vs 15.9 ± 4.7, P <0.05) and TT level (［16.7 ± 2.2］ vs ［13.1 ± 2.8］ nmol/L, P <0.05), but a decreased AMS score (20.7 ± 5.7 vs 31.3±6.5, P <0.05).@*CONCLUSIONS@#TUC combined with Qilin Pills has a better effect and a lower rate of adverse reactions than TUC used alone in the treatment of late-onset hypogonadism in males.

Progress on the Rule of Clavicle Epiphyseal Closure Using Multi-Imaging Technology / 法医学杂志

People aged 18 years could be punished lightly or diminished criminal responsibility, even be spared the death sentence, which has important meaning in Chinese judicatory adjudication. The epiphysis of long bones from human limbs and the secondary sexual characteristics almost have developed completely before 18 years old. Clavicle epiphysis is one of the articular metaphysis which has a late epiphyseal closure. The recent studies in exploring the rule of clavicle epiphyseal by multi-imaging technology shows that the development of clavicle epiphysis has some value in age estimation of 18 years old. CT, especially thin-section CT, is widely used at present. However, thin-section CT scanning has great net radiation, which is not ethically acceptable if it is not for diagnosis and treatment. MRI is nonradioactive tomographic imaging and easy to evaluate, which is one of the future research directions in forensic age estimation using the medial clavicle. This paper summarizes the progress on the rule of clavicle epiphyseal closure, and analyzes and summarizes the feasibility of rule of clavicle epiphyseal closure applies on age estimation.

Effects and mechanism of low-intensity pulsed ultrasound on extracellular matrix in rabbit knee osteoarthritis / 中国骨伤

<p><b>OBJECTIVE</b>To observe the effects of low-intensity pulsed ultrasound (LIPUS) on repairing extracellular matrix in rabbit knee osteoarthritis and analyze its mechanism.</p><p><b>METHODS</b>Sixty adult female rabbits with an average weight of (2.0 ± 0.2) kg, were divided randomly into two groups (experimental group and control group, 30 rabbits in each group). All rabbits were replicated in right knees by Hulth method for knee osteoarthritis model. Two weeks after operation, the rabbits in experimental group were treated with LIPUS, and the ultrasonic frequency was (800 ± 5%)KHz and the maximum intensities of spatially averaged and time averaged (SATA) was (50 ± 10%) mw/cm2, for 1 time a day and every time 20 min, while the rabbits in control group were treated with sham LIPUS,the same operation with experimental group but without energy output. At the 2, 4, 8 weeks after treatment, 10 rabbits in each group were randomly killed for each time. The general changes of cartilage and its histopathological changes by HE staining were observed; the expression of collagen type II, proteoglycan, MMP-3, 7, 13 in cartilage were analyzed by immunohistochemical and RT-PCR technique; and the expression of NO in cartilage was analyzed by nitrate reduction method.</p><p><b>RESULTS</b>On the same observed time point, the damage degree of cartilage in experimental group was slighter than that of control group (P < 0.01), the expression of MMP-3, 7, 13 and NO in cartilage in experimental group was lower than that of control group (P < 0.01) while collagen type II and proteoglycan was higher than that of control group (P < 0.01).</p><p><b>CONCLUSION</b>Low-intensity pulsed ultrasound can repair the damaged cartilage by reducing the expression of MMP-3, 7, 13, inhibiting the secretion of NO and promoting the synthesis of collagen type II and proteoglycan in cartilage.</p>

Genome-wide linkage scan for an ethnic Han Chinese pedigree affected with schizophrenia / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To perform genome-wide linkage analysis for an ethnic Han Chinese pedigree with schizophrenia in order to locate the susceptibility genes.</p><p><b>METHODS</b>Genomic DNA was extracted from 4 mL of peripheral blood using conventional phenol-chloroform method. Illumina Infinium Linkage 24 BeadChips chip was used for determining the genotypes through detection of single nucleotide polymorphisms (SNPs). After processing the raw data using Illumina BeadStudio software, two-point nonparametric linkage analysis and two-point parametric linkage analysis were performed with Merlin software.</p><p><b>RESULTS</b>By two-point nonparametric linkage analysis, 27 sites with high LOD scores (LOD=0.63-0.75, P U+003C 0.05) were identified. Among these, 3 SNPs(rs993694, rs992690 rs1861577) were located in 12p12.3 region, whilst the remainders were located in 4p12-q22 region. Two-point parametric linkage analysis under a dominant model has yielded almost identical results.</p><p><b>CONCLUSION</b>Chromosomal regions 4p12-q22 and 12p12.3 probably contain susceptibility genes for schizophrenia.</p>

Skeletal age estimation by pelvic X-ray of Han teenagers in Sichuan / 法医学杂志

OBJECTIVE@#To estimate the chronological age of Han teenagers in Sichuan by the epiphyses development characteristics of iliac crest and ischial tuberosity in X-ray digital images.@*METHODS@#According to TW2 classification principle, combining with the age range of our subjects and epiphyses development regularity, the degree of epiphyses development of iliac crest and ischial tuberosity had been divided into 8 grades (0-7) and 7 grades (0-6), respectively. Based on the degrees, the pelvic X-rays digital images of 691 samples of 16-20 aged teenagers were read and statistically analyzed by SPSS 19.0 software. The multiple linear regression equation was established by skeletal age (Y), development degree of iliac crest epiphyses (X1) and ischial tuberosity epiphyses (X2).@*RESULTS@#There were no statistical differences between two sides of epiphyses development of iliac crest and ischial tuberosity (P>0.05). There were no statistical difference between genders (P>0.05). The re-substitution method of the samples showed that the accuracy of equation as Y=15.269+0.444X1+0.236 X2 was high. The result of the re-substitution method showed a high accuracy of the equation.@*CONCLUSION@#The multiple linear regression equation could be used to identify the skeletal age for providing the scientific basis to identify the 18-year-old Han population in Sichuan.

Stature estimation for Sichuan Han nationality female based on X-ray technology with measurement of lumbar vertebrae / 法医学杂志

OBJECTIVE@#To establish the mathematical models of stature estimation for Sichuan Han female with measurement of lumbar vertebrae by X-ray to provide essential data for forensic anthropology research.@*METHODS@#The samples, 206 Sichuan Han females, were divided into three groups including group A, B and C according to the ages. Group A (206 samples) consisted of all ages, group B (116 samples) were 20-45 years old and 90 samples over 45 years old were group C. All the samples were examined lumbar vertebrae through CR technology, including the parameters of five centrums (L1-L5) as anterior border, posterior border and central heights (x1-x15), total central height of lumbar spine (x16), and the real height of every sample. The linear regression analysis was produced using the parameters to establish the mathematical models of stature estimation. Sixty-two trained subjects were tested to verify the accuracy of the mathematical models.@*RESULTS@#The established mathematical models by hypothesis test of linear regression equation model were statistically significant (P<0.05). The standard errors of the equation were 2.982-5.004 cm, while correlation coefficients were 0.370-0.779 and multiple correlation coefficients were 0.533-0.834. The return tests of the highest correlation coefficient and multiple correlation coefficient of each group showed that the highest accuracy of the multiple regression equation, y = 100.33 + 1.489 x3 - 0.548 x6 + 0.772 x9 + 0.058 x12 + 0.645 x15, in group A were 80.6% (+/- lSE) and 100% (+/- 2SE).@*CONCLUSION@#The established mathematical models in this study could be applied for the stature estimation for Sichuan Han females.

Survivin gene silencing inhibits the proliferation of human gastric cancer MGC-803 cells and enhances their sensitivity to celecoxib / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the effect of small interfering RNA (siRNA)-mediated survivin knock-down on the proliferation of human gastric cancer MGC-803 cells and their sensitivity to celecoxib.</p><p><b>METHODS</b>The siRNA against survivin was constructed and transfected into MGC-803 cells via Lipofectamine(TM) 2000. The expression of survivin in the transfected cells was detected by RT-PCR and Western blot, and flow cytometry was used to detect the cell cycle changes. The sensitivity of the cells to celecoxib after transfection was examined using MTT assay and clonogenic assay.</p><p><b>RESULTS</b>The protein and mRNA levels of survivin in MGC-803 cells were decreased significantly after siRNA transfection, which also caused cell cycle arrest in G(0)/G(1) phase. The sensitivity of MGC-803 cells to celecoxib was significantly increased after siRNA transfection.</p><p><b>CONCLUSION</b>siRNA-mediated survivin silencing causes growth suppression of MGC-803 cells and enhances their sensitivity to celecoxib in vitro.</p>

Relationship between the increase of fibrous and fatty in atrioventricular node and narrowing of the atrioventricular node artery / 法医学杂志

OBJECTIVE@#To explore relationship between increase of fibrous and fatty in atrioventricular node (AVN) and narrowing of the AVN artery. To analyze the cause of pathological fibrosis and fatty infiltration in AVN.@*METHODS@#One hundred and nineteen cases of sudden cardiac death determined by autopsy were selected and the histological sections were examined with Image-pro plus software to calculate the AVN area, AVN artery inside-diameter, AVN artery lumen area(LA) , AVN artery perimeter area(PA), fibrous area and fatty area. All cases were divided into two groups: narrowing of artery group and normal control group. The changes of the PA/LA value and the fibrous and fatty contents were evaluated.@*RESULTS@#The PA/LA value is the highest in 21-40 age group. The difference of the fatty contents and total interstitial tissue was statistical significance in the two groups under 40 years of age.@*CONCLUSION@#There is some relationship between the narrowing of the AVN artery and the increase of interstitial contents in AVN.

Study on rhGM-CSF as adjuvant in revaccination among adults of non-and hyporesponders to hepatitis B vaccine / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To investigate the effect of recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) as adjuvant on immune response in adults of non-and hyporesponders to hepatitis B vaccine.</p><p><b>METHODS</b>Those who were once immunized with recombined yeast gene hepatitis B vaccine more than one standard scheme in two years and negative for hepatitis B markers were randomly sorted as group A and group B. 33 adults of group A were given hepatitis B vaccine 10 microg each time. The immune procedure was 0, 1 and 6 month. 34 adults of group B were given rhGM-CSF 300 microg for the first day, then 10 microg each time for routine immune. The blood samples were collected before the first injection and in 1, 2 and 8 months (T1, T2, T8) following the first injection to test Anti-HBs.</p><p><b>RESULTS</b>Anti-HBs positive conversion rates of group A and B at T8 was 39.39% and 64.71% respectively (P = 0.038). Anti-HBs levels of group B at T1, T2, T8 were (113.85 +/- 198.56) mIU/ml, (312.40 +/- 349.44) mIU/ml, (427.74 +/- 411.58) mIU/ml (P = 0.001). There was significant difference between group A and B in T8 Anti-HBs levels (P = 0.010).</p><p><b>CONCLUSION</b>Better immune response was found in the group of rhGM-CSF with hepatitis B vaccine. So rhGM-CSF can induce the immune respond to hepatitis B vaccine.</p>

Traffic accidents associated with emotional stress after divorce / 法医学杂志

In recent years, the traffic accidents increased gradually, especially those caused by the drivers daily emotional abnormality and in which the drivers were liable. This article reviewed the traffic accidents caused by divorced driver's emotional abnormality, illustrated the features of those accidents from the gender, age, occupation and mileage of drivers. It was considered that the major cause of those accidents was excessive drinking due to drivers' emotional stress. Suggestions about preventing the traffic accidents caused by emotional abnormality were put forward so as to make the corresponding rules and finally decrease the emotional abnormality traffic accidents.

In vitro study on bFGF expression of rat astrocytes after mechanical injury / 法医学杂志

OBJECTIVE@#To study the alteration of basic fibroblast growth factor (bFGB) expression in astrocytes in vitro after mechanical injury and to understand the repair mechanism of brain injury.@*METHODS@#Astrocytes were isolated from cerebral cortex of SD rats born in 24 hours, and then cultured and purified. The cultured astrocytes were randomly divided into control group and injury groups that were subjected to mechanical injury at 30 min, 1h, 3h, 6h, 12h, 24h, 3d, and 7d. The levels of bFGF expression in the astrocytes after injury were detected by ABC immunohistochemistry.@*RESULTS@#More than 95% of the cultured cells were astrocytes. The levels of bFGF expression werevery low in the control group. On the other hand, increased levels of bFGF expression could be observed at 1-3h after injury. The expression levels increased significantly at 6-12h, reached peak level at 24h, remained at the high level up to 3 days, and the decreased gradually.@*CONCLUSION@#The changes of bFGF expression levels in cultured astrocytes in vitro after mechanical injury are similar to that observed in vivo experimental model, both of which show time-dependant characteristic, with only slightly earlier expression of bFGF observed in vitro. Thus, the expression of bFGF after injury can be one of evidences for estimation of brain injury intervals. the cell injury model in vitro may have superiority in the study of the molecule mechanism of tissue and cell injury.

Stature estimation from length of tibias and fibulas measured in computed radiography of living body / 法医学杂志

OBJECTIVE@#By measuring lengths of tibias and fibulas in computed radiography (CR) of living body, regression formulae for stature estimation were obtained from these measurements that were adapted to the population of northern adults of Han.@*METHODS@#The length of tibias and fibulas were measured in radiography, including 422 subjects (207 men and 215 women). The body height of each subject was also recorded. Linear regression analysis between body height and the length of tibias and fibulas , analysis of variance of hypothesis test of linear regression model and t-test of the partial regression coefficient were applied to establish the regression equations for stature estimation. Then the paired t-test between the predictive value and the actual body height, the residual analysis, the tests of equal scedasticity and colinearity had been done to evaluate whether the equations accord with the model.@*RESULTS@#Thirty five regression equations which were statistical significant were obtained. The multiple correlation coefficient was from 0.909 to 0.823 and the standard error of the estimation was between 2.48-3.87 cm.@*CONCLUSION@#The lengths of tibias and fibulas on the CR films could be helpful to estimate stature. These equations could be used to estimate the body height of the Han population in North China.

Sex determination by discriminant analysis of calcaneal measurements on the lateral digital radiography / 法医学杂志

OBJECTIVE@#Five measurements of the calcaneus taken on digital radiography (DR) of adults of Han Population of Sichuan Province were selected to determine sex by multivariate discriminant analysis.@*METHODS@#Lateral radiographs of calcaneus taken from 393 subjects were collected. The samples were randomly divided into the experimental group (148 males and 186 females) and the examined group (26 males and 33 females). Five measurements were taken from the radiography. The analysis of variance (AVON) was carried out to determine if there was significant difference between the male and female. The discriminant functions were drawn by Fisher discriminant analysis. The effects of all obtained functions were evaluated with the examined samples.@*RESULTS@#There was statistically significant difference in the five measurements between the males and the females (P<0.05). Six groups of discriminant functions were obtained with an accuracy ranged from 78.4% to 88.9%. When applied on the examined samples, the sex discriminant accuracy varied from 79.7% to 86.4%.@*CONCLUSION@#These five measurements acquired from the lateral radiographs of calcaneus could be used for sex assessment during forensic identification of individuals.

Retinal examination with ophthalmic endoscopy in forensic autopsy / 法医学杂志

Autopsy by forensic pathologist is a main mean currently to determine the cause of sudden unexpected death. Retinal examination is important but seldom performed during a forensic autopsy for various reasons. The value of retina examination has not been recognized. With invention of ophthalmic endoscopy and its subsequent application in postmortem retina examination, it has proved to be useful adjunct to determine the cause of death and to estimate the postmortem interval.

The prospect of application of toxicogenetics/pharmcogenetics theory and methods in forensic practice / 法医学杂志

Individual response to drugs, toxicants, environmental chemicals and allergens varies with genotype. Some respond well to these substances without significant consequences, while others may respond strongly with severe consequences and even death. Toxicogenetics and toxicogenomics as well as pharmacogenetics explain the genetic basis for the variations of individual response to toxicants by sequencing the human genome and large-scale identification of genome polymorphism. The new disciplines will provide a new route for forensic specialists to determine the cause of death.

Stature estimation from upper extremity long bones by digital radiography / 法医学杂志

OBJECTIVE@#To obtain regression formula for estimation of stature in Han population in Sichuan Province from length of the upper extremity long bones by digital radiography.@*METHODS@#The statures of 365 healthy adults and digital radiographs of their right upper extremity long bones were measured. All statistical dispositions were done in SPSS including description and regression analysis.@*RESULTS@#Twenty-seven simple regression and 3 multiple regression formulae with statistic significance in estimation of the stature from the sum of the length of the upper extremity long bones were established. Regression coefficient was higher in ulna than in radius, in male than in female, and was more accurate in multiple regression formulae than in simple regression formulae.@*CONCLUSION@#Digital radiograph measurement of the length of the upper extremity long bones seems to be a simple and practical method to estimate human stature in forensic practice.

The application of cerebral angiography in the forensic postmortem examination / 法医学杂志

As a "Gold Standard" in clinical diagnosis of cerebrovascular disease, cerebral angiography also plays an important role in forensic postmortem examination. The key roles of identifying the site of cerebrovascular disease and the cause of death as well as providing guidance for autopsy and postmortem sampling and the broad future application of cerebral angiography in forensic practice are reviewed.

Expression of caspase-8 after brain injury from fluid percussion in rats / 法医学杂志

OBJECTIVE@#To provide the evidence of the relationship between brain injury and the time of injury.@*METHODS@#Rats were contused on brain by fluid percussion, then were killed after injury for 15 min, 30 min, 1,3,6,12 h, and 1,4,7,14 d respectively. The expression of caspase-8 were detected by immunohistochemical technology on rat brain section and the results were assessed by image analysis system in the cerebral cortex, thalamus, and hippocampus.@*RESULTS@#The expression of caspase-8 in cortex and hippocampus could be detected in 30 min after injury, increased significantly in 3h, reached apex in 1d after injury, remained 4d before decreased. In addition, the expression of caspase-8 can be detected in 1h after injury and reached apex in 1d after injury, and remained 4d then reduced.@*CONCLUSION@#It seems that the expression of caspase-8 should be a useful target for diagnosis of early brain injury.

The clinical forensic medicine identification on pseudoseizures after head trauma / 法医学杂志

Two pseudoseizures after head trauma are reported. We summarize the clinical manifestation of pseudoseizures, and identify difference of pseudoseizures and Seizures after head trauma. The forensic identification of pseudoseizures after head trauma should base on the extend and position of cerebral trauma, sequelae focus in cerebral, and the clinical manifestation.

Epidemiology of assaultive injuries in areas of Sichuan province, China / 中华创伤杂志(英文版)

<p><b>OBJECTIVE</b>To scrutinize the epidemiological characteristics of assaultive injuries in Sichuan province, China.</p><p><b>METHODS</b>A survey of all cases of assaultive injuries reported by police was performed during 8 years in eight counties of Sichuan province, China. A total of 2862 victims and 2856 offenders were registered.</p><p><b>RESULTS</b>The majority of victims and offenders were young men at the age of 20-39 and only received an education at secondary school or primary school. The largest fraction of these cases took place at farm or by-place during 10.00-11.00 o'clock, 16.00-17.00 o'clock and 20.00-21.00 o'clock. The tangles caused by trifles were the most common factors inducing assaultive injuries and accounted for 42.1 percent of the causes of assaults. Blunt injuries were mainly caused by punching (40%) and kicking (17.2%). About 37.3% of the lesions seriously happened in the regions of face and head. Open wounds accounted for 40.3% of these different injuries.</p><p><b>CONCLUSIONS</b>It is valuable to take some specific measures to prevent and control assaultive injuries according to their territorial characteristics.</p>