ABSTRACT
Objective: To observie the compatibility of rat adipose-derived stem cells (ADSCs) and three dimensional printing (3DP) gelatin scaffolds crosslinked with glutaraldehyde, the most suitable for cell growth aperture, in order to provide experimental basis for constructing tissue-engineered tissues or organs. Methods: The enzyme digestion method was adopted to separate and extract rat ADSCs. Flow cytometry and multi-directional differentiation method were used for identification. The ADSCs were cultured with 3DP gelatin scaffolds of different aperturs. Scanning electron microscopy (SEM) and transmission electron microscope (TEM) were used to observe the ultra-microstructure. The cell vitality analyzer was used to detect its survival rate. MTT method was used to detect the effects of two dimensional (2D) and three dimensional (3D) cultural methods on ADSCs cell vitality. Results: The seeding ADSCs had multi-directional differentiation potential, and had the basic characteristics of stem cells. After inoculation of ADSCs to 3DP gelatin scaffolds, cells were oval or spindle observed by SEM, which was different from the traditional 2D culture form. The cells scattered within the scaffold gap, the structures such as the nucleus and organelles were clear, which demonstrated a good compatibility with scaffold. The cell survival rate of 90p.m aperture scaffold was the highest, and 3D cultural method was more advantageous to maintain the vitality of ADSCs. Conclusion: The compatibility of ADSCs with 3DP gelatin scaffold is good, and 90μm aperture scaffold is most suitable for the growth of ADSCs.
ABSTRACT
A nutrient solution experiment was conducted to investigate the effect of Fe and Zn supply on Fe, Zn, Cu, and Mn concentrations in wheat plants. The experiment used a factorial combination of two Fe levels (0 and 5 mg l-1) and three Zn levels (0, 0.1 and 10 mg l-1). The supply of Fe (5 mg l-1) and Zn (0.1 mg l-1) increased plant dry weight and leaf chlorophyll content compared to the Fe or Zn deficient (0 mg l-1) treatments. However, excess Zn supply (10 mg l-1) reduced plant dry weights and leaf chlorophyll content. Iron supply (5 mg l-1) reduced wheat Zn concentrations by 49%, Cu concentrations by 34%, and Mn by 56% respectively. Zinc supply (10 mg l-1) reduced wheat Fe concentrations by an average of 8%, but had no significant effect on Cu and Mn concentrations. Stepwise regression analyses indicated that Zn, Cu, and Mn concentrations were negatively correlated with root- and leaf-Fe concentrations, but positively correlated with stem-Fe concentrations. Leaf-Mn concentrations were negatively correlated with root-, stem- and leaf-Zn concentrations.