ABSTRACT
The rare edible and medicinal fungus Antrodia cinnamomea has a substantial potential for development. In this study, Illumina HiSeq 2000 was used to sequence its transcriptome.The results were assembled de novo, and 66,589 unigenes with an N50 of 4413 bp were obtained. Compared with public databases, 6,061, 3,257, and 2,807 unigenes were annotated to the Non-Redundant, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases, respectively. The genes related to terpene biosynthesis in the mycelia of A. cinnamomea were analyzed, and acetyl CoA synthase (ACS2 and ACS4), hydroxymethylglutaryl CoA reductase (HMGR), farnesyl transferase (FTase), and squalene synthase (SQS) were found to be upregulated in XZJ (twig of C. camphora) and NZJ (twig of C. kanehirae). Moreover, ACS5 and 2,3-oxidized squalene cyclase ( OCS) were highly expressed in NZJ, while heme IX farnesyl transferase (IX-FIT) and ACS3 were significantly expressed in XZJ. The differential expression of ACS1, ACS2, HMGR, IX-FIT, SQS, and OCS was confirmed by real-time quantitative reverse transcription PCR. This study provides a new concept for the additional exploration of the molecular regulatory mechanism of terpenoid biosynthesis and data for the biotechnology of terpenoid production.
ABSTRACT
Objective To obtained the gene AcPKS1 of Antrodia camphorata, analyze using bioinformatics, and detect the condition of expressing in the different medium. Methods Polyketide synthases gene was obtained from the genome of A. camphorata through analyzing the genome, and the full length of the gene was obtained through designed the special primers including initiation codon and termination codon and the template using cDNA of A. camphorata, which named the gene AcPKS1, and using the bioinformatics analysis and expression profiles analysis in the different medium. Results The full length of AcPKS1 gene was 6 348 bp, including six introns and seven exons, and the expression region encoded 2 115 amino acids; the bioinformatics analysis showed that AcPKS1 was a kind of nonreduced PKS of type in fungi, the domains was SAT-KS-PT-ACP-ACP-TE, and the enzyme catalyzed a new kind of cyclization in the process of polyketides biosynthesis; The expression profiles revealed that glucose was necessary during the expression of AcPKS protein, and the expression quantity of the AcPKS1 protein basically proportion to the content of glucose. Conclusion The result of this text has applied foundation to identify the polyketide synthase gene and take full advantage genomic resources of A. camphorata.