ABSTRACT
Purpose To investigate the expression of cy-toskeletal protein 4. 1N in Ewing's sarcoma and the correlation with cell proliferation as well as clinicopathological characteris-tics. Methods Immunohistochemical EnVision two-step stai- ning was used to detect the expression of 4. 1N in 52 Ewing's sarcoma cases, which 47 cases of them were performed to detect the expression of Ki-67. The relationship between the expression of 4. 1N and Ki-67, and the correlation with the pathological fea- tures including prognosis were analyzed. Results The positive expression of 4. 1N was found in 35% (18/52) of Ewing's sar-coma. Lower frequency of 4. 1N strong expression (1. 9% , 1/52) was detected in the cohort. Ki-67 labeling index in Ewing's sarcoma varied from 3% to 85% . 4. 1N expression displayed a negative correlation with Ki-67 index ( P ＜0. 05). Among the follow-up of 27 cases, Kaplan-Meier survival analysis suggested that patients with 4. 1N-negative had shorter survival period than patients with 4. 1N positive expression (P ＜0. 05). However, the expression of 4. 1N was not statistically correlated with sex, age, tumor's localization and metastasis. Conclusion 4. 1N mainly showed negative or lower expression in Ewing's sarcoma and was related to cell proliferation index Ki-67, which implied a potential function in regulating proliferation in Ewing's sarcoma. 4. 1N expression has potentially predictive value in outcome of E-wing's sarcoma.
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of 25-hydroxyvitamin D3 on the permeability and ZO-1 expression in normal human airway epithelial cells.</p><p><b>METHODS</b>MTT assay was used to assess the viability of human airway epithelial cell line 16HBE following a 24-hour exposure to different concentrations of 25-hydroxy vitamin D3, and the transepithelial electrical resistance (TER) of the cell monolayer was measured using a Millicell-ERS voltohmmeter. Real-time quantitative RT-PCR was employed to determine the changes of ZO-1 mRNA expression in the cells following the exposures.</p><p><b>RESULTS</b>Exposure to 25-hydroxyvitamin D3 resulted in significantly increased permeability of 16HBE cells, but the exspression of ZO-1 showed no obvious changes. 25-hydroxyvitamin D3 at 4×10(-9) mol/L showed the strongest effect in increasing the permeability of cell monolayer.</p><p><b>CONCLUSION</b>25-hydroxyvitamin D3 increases the permeability of normal bronchial airway epithelial cell monolayer in vitro, but this effect is not mediated by upregulation of ZO-1 expression.</p>