ABSTRACT
<p><b>OBJECTIVE</b>To study the pharmacokinetic of 10-Hydroxycamptothecin HCPT by intraperitoneal administration.</p><p><b>METHOD</b>Six dogs were given HCPT by intraperitoneal perfusion: HCPT 2 mg x kg(-1), dissolved in 300 mL NS. During the course of experiments, peritoneal fluids and blood were sampled using a standardized protocol. The concentration of HCPT in all samples was determined by high performance liquid chromatography (HPLC).</p><p><b>RESULT</b>The area under the curve (AUC) of peritoneal fluids was significantly highly as compared to the AUC of femoral vein and portal vein (P < 0.001).</p><p><b>CONCLUSION</b>These experiments demonstrate that intraperitoneal chemotherapy has significant effect on the pharmacokinetics of IP route of HCPT administration. This method should be a more reasonable chemotherapy for the prevention of recurrence and liver metastasis of gastric cancer, colon cancer and ovary cancer after radical resection.</p>
Subject(s)
Animals , Dogs , Male , Antineoplastic Agents, Phytogenic , Pharmacokinetics , Area Under Curve , Ascitic Fluid , Metabolism , Camptothecin , Pharmacokinetics , Infusions, ParenteralABSTRACT
Anthocyanidin is a type of the plant pigments distributed very extensively, in traditional Chinese herbal products as well. In this review was introduced the recently progress in the anti-cancer trials of anthocyanidins, including the anti-oxidation, the prevention of DNA strand scission, stimulation of cell differentiation, induction of cell apoptosis, interference of regulation of cell proliferation, anti-angiogenic property etc, and the research of anti-cancer mechanisms of anthocyanidin and its structure-activity relationship, pointed the foreground of research and development of anti-cancer medicine.
Subject(s)
Animals , Humans , Anthocyanins , Chemistry , Pharmacology , Antineoplastic Agents, Phytogenic , Chemistry , Pharmacology , Antioxidants , Pharmacology , Apoptosis , Cell Differentiation , DNA Fragmentation , Neoplasms , Pathology , Neovascularization, Pathologic , Plants, Medicinal , Chemistry , Structure-Activity RelationshipABSTRACT
Objective To study the changes of insulin- like growth factor- 1(IGF-1)in blood and cerebrospinal fluid (CSF) in children with viral encephalitis (VE).Methods The IGF-1 levels in blood and CSF were determined before treatment by ELISA in 25 children who admitted with VE, including 15 cases with severe VE and another 10 cases with mild VE, 10 children served as con-trols. Results Before treatment, the blood IGF-1 levels in VE group were significantly lower than those of controls, but the CSF IGF-1 levels were significantly higher than those of controls(P0.05), but the blood IGF-1 levels in serve VE group were significanfly lower than those of mild VE group and controls(P
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of parthenolide on the proliferation of vascular smooth muscle cell(VSMC) and its mechanism.</p><p><b>METHOD</b>Vascular smooth muscle cell was cultured, the protein levels of c-fos, c-myc, p15, p16, p18, p19 were measured by Western blot method, cell cycle were examined with flow cytometry, and the DNA synthesis was determined by [3H]-TdR incorporation.</p><p><b>RESULT</b>Parthenolide inhibited protein levels of c-fos, c-myc in a time-dependent manner but didn't affect the protein levels of p15, p16, p18, p19. Flow cytometric DNA analysis revealed that parthenolide increased significantly G0/G1 phase of VSMC and decreased S phase of VSMC in a dose-dependent manner. Parthenolide inhibited [3H]-TdR incorporation in a dose dependent manner.</p><p><b>CONCLUSION</b>Parthenolide may inhibit proliferation of VSMC by inhibiting the expressions of c-fos, c-myc, but not the expressions of p15, p16, p18, p19.</p>
Subject(s)
Animals , Rats , Aorta, Thoracic , Cell Biology , Asteraceae , Chemistry , Cell Cycle , Cell Division , Cells, Cultured , Muscle, Smooth, Vascular , Cell Biology , Myocytes, Smooth Muscle , Cell Biology , Metabolism , Plants, Medicinal , Chemistry , Proto-Oncogene Proteins c-fos , Metabolism , Proto-Oncogene Proteins c-myc , Metabolism , Rats, Sprague-Dawley , Sesquiterpenes , PharmacologyABSTRACT
<p><b>OBJECTIVE</b>To study action of Cryptoporus volvatus ferment substance (CVFS) on leukotriene production of polymorphonuclear leukocytes in rats.</p><p><b>METHODS</b>The level of slow reaction substance (SRS) and leukotriene B4 (LTB4) in polymorphonuclear leukocytes (PMNs) in rats in vitro were determined with bioassay and HPLC.</p><p><b>RESULTS</b>CVFS 0.9, 2.7 g.kg-1 by ig significantly inhibited SRS and LTB4 production in PMNs in rats in vivo.</p><p><b>CONCLUSION</b>The inhibition effect of CVFS on SRS and LTB4 release may be related to its mechanism of anti-inflammation and anti-asthma.</p>