ABSTRACT
AIM:To investigate effects of resveratrol on mitochondrial DNA copy in retinal vascular endothelial cells cultured under high glucose conditions and its mechanism.METHODS:Human retinal vascular endothelial cells were cultured in low glucose or high glucose medium,the genomic DNA was extracted and copy number of mitochondrial DNA was detected by real-time PCR.Effects of resveratrol on the mitochondrial DNA copy in retinal vascular endothelial cells cultured under high glucose medium were studied.The expression of mitochondrial transcription factor A (TFAM) and acetylated proliferator-activated receptor coactivator-1 alpha (PGC-1α) were analyzed by Western blot and coimmunoprecipitation.RESULTS:High glucose inhibited the copy number of mitochondrial DNA in retinal vascular endothelial cells.However,resveratrol decreased the level of acetylated PGC-1α in retinal vascular endothelial cells,increased the expression of TFAM and the copy number of mitochondrial DNA.CONCLUSION:Resveratrol may improve mitochondrial function of retinal vascular endothelial cells exposed to a high glucose environment via activation of the PCG-1α-TFAM signaling pathway.
ABSTRACT
OBJECTIVE@#To seek a method of age determination.@*METHODS@#This sample consists of 69 female Chinese han individuals whose ages been known. Five positions were selected from their radiograms which have been divided into three or six grade and then set up a mathematical model to deduce age by multiple linear regression analysis.@*RESULTS@#The standard deviation of female thyroid cartilage is 1.969 and the multiple correctation coefficient is 0.984.@*CONCLUSION@#The observation proved that age determination by radiographic feature of thyroid cartilage can be used for female Chinese han population from 18 to 60 years old.
Subject(s)
Adolescent , Adult , Female , Humans , Middle Aged , Age Determination by Skeleton/methods , Asian People , Forensic Medicine , Linear Models , Thyroid Cartilage/diagnostic imagingABSTRACT
OBJECTIVE@#To seek a exact method of age determination.@*METHODS@#This sample consists of 73 male Chinese han individuals whose ages have been known. Eight positions were selected from their radiograms which have been divided into three or six grade and then set up a mathematical model to deduce age by multiple linear regression analysis.@*RESULTS@#The standard deviation of male thyroid cartilage is 1.854 and the multiple correctation coefficient is 0.9971.@*CONCLUSION@#The observation proved that age determination by radiographic feature of thyroid cartiage may be suitable for male Chinese han population from 13 to 67 years old.