ABSTRACT
The incidence rate of nonalcoholic fatty liver disease (NAFLD) is increasing year by year, with limited treatment methods, and its pathogenesis is a research hotspot at present. In order to better clarify its pathogenesis, it is urgent to develop advanced, safe, and effective in vitro or in vivo models to understand and develop treatment strategies for this disease. This article reviews the in vitro models commonly used in the preclinical study of NAFLD and discusses their advantages and disadvantages, so as to provide a theoretical basis for the pathogenesis and treatment of NAFLD.
ABSTRACT
Objective:To evaluate the effect of thermocycling on nano-silver-incorporated silicone denture liner for Candida albicans (CA)adhesion.Methods:Specimens(1 0 mm ×1 0 mm ×3 mm)of silicone denture liner(control)and that incorporated with nano-sil-ver were prepared according to the manufacturer's direction.8 of the each kind of specimens were immersed in distilled water for 24 h at 37 ℃,and 8 were subjected to thermocycling for 4 000 cycles at temperatures ranging from (5 ±1 )℃to (55 ±1 )℃with a 60 s dwell time per temperature.In vitro CA adhesion assay was used to measure the levels of adherence to the specimens.Data were analysed sta-tistically.Results:Thermocycling increased CA adhesion to control specimens(P0.05 ).The adhesion of CA to nano-silver incorporated specimens was fewer than that to the controls with or wihout themocycling(P<0.01 ).Conclusion:Nano-silver incorporation may inhibit CA adhesion to silicone denture liner.Ther-mal cycling has no influence on C.albicans adhesion to silicone denture liner incorporated nano-sliver.
ABSTRACT
16 standered acrylic resin blocks with silicone resilient denture liner(10 mm ×8 mm ×3 mm)incorporated with nano-silver par-ticles at 5% (m/m,by dry wet)in-between were prepared.8 blocks were immersed in distilled water for 24 h at 37 ℃ as the controls (group 1).Another 8 were thermocycled for 4 000 cycles(group 2).16 acrylic resin bars were thermal cycled for 4 000 cycles.Then they were processed exactly as the control group (group 3).Tensile bond strength of the specimens was measured by a universal testing machine. The bond strength values(MPa)of group 1,2 and 3 were 2.683 ±0.435,2.179 ±0.633 and 1.460 ±0.566 respectively(group 1 vs 2,P>0.05;group 3 vs 1 or 2,P<0.05).The failure mode of the blocks in group 1 and 2 was mainly cohesive failure,in group 3 adhesion fail-ure.