Association between the expression of somatostatin receptors and pathological features, prognosis in hepatocellular carcinoma / 临床肝胆病杂志

Objective To investigate the expression of somatostatin receptors (SSTRs) in hepatocellular carcinoma (HCC) tissue and its association with clinicopathological features and prognosis of HCC.Methods HCC samples were collected from 80 patients who visited Third Hospital of PLA and Department of Hepatobiliary Surgery in The Second Affiliated Hospital of Dalian Medical University and who underwent hepatectomy from July 2012 to December 2014 and were diagnosed with HCC based on postoperative pathology (trial group).Another 80 patients who were suspected of liver disease and were not diagnosed with HCC by liver biopsy were enrolled as control group.RT-PCR and immunohistochemistry were used to measure the mRNA and protein expression of SSTR-2 and SSTR-3.The t-test was used for comparison of continuous data between groups,the chi-square test was used for comparison of categorical data between groups,the Kaplan-Meier method was used to analyze patients' survival,and the Cox regression analysis was used to investigate the influencing factors for the prognosis of HCC patients.Results The control group had significantly higher mRNA and protein expression of SSTR-2 and SSTR-3 than the trial group (t =6.456 and 8.128,x2 =7.992 and 9.157,all P ＜ 0.05).The univariate analysis showed that the mRNA expression of SSTR-2 and SSTR-3 was significantly correlated with tumor nodule (t =6.533 and 5.041,both P ＜ 0.05),degree of tumor differentiation (t =4.672 and 4.013,both P ＜ 0.05),depth of infiltration (t =6.735 and 7.019,both P ＜ 0.05),viral hepatitis (t =4.929 and 4.535,both P ＜ 0.05),alcoholic hepatitis (t =4.032 and 4.362,both P ＜ 0.05),and diabetes (t =4.372 and 6.293,both P ＜ 0.05),and the protein expression of SSTR-2 and SSTR-3 was significantly correlated with tumor nodule (x2 =25.223 and 15.399,both P ＜ 0.05),degree of tumor differentiation (x2 =7.535 and 10.944,both P ＜ 0.05),and depth of infiltration (x2 =22.520 and 9.968,both P ＜ 0.05).Compared with the group with positive expression of SSTR-2 and SSTR-3,the group with negative expression had significantly lower cumulative postoperative disease-free survival rate (P =0.015 and 0.004) and postoperative overall survival rate (P =0.009 and ＜ 0.001).The Cox model analysis showed that protein expression of SSTR-2 and SSTR-3,the number of tumor nodules,liver cirrhosis,and vein infiltration in HCC tissue were independent risk factors for overall survival after HCC surgery (P ＜ 0.05).Conclusion HCC patients have lower expression of SSTR-2 and SSTR-3 than non-HCC patients,and such low expression is closely associated with invasion/metastasis and poor prognosis of HCC.SSTRs may be the markers for the prognosis of HCC.

Effect of oxygen-vectors on the production of ε-poly-L-lysine / 生物工程学报

To enhance the production of ε-poly-L-lysine (ε-PL) by improving dissolved oxygen level of the fermentation system, different oxygen-vectors were added to broth and n-dodecane was screened as the best oxygen-vector. The best amount of n-dodecane was 0.5% (V/V) and the best time was at start of the fermentation. In a fed-batch fermentation in a 5 L bioreactor, ε-PL concentration reached a maximum of (30.8 ± 0.46) g/L and the dry cell weight obtained was (33.8 ± 0.29) g/L, increasing by 31.6% and 20.7% compared with the control group, respectively. This improvement can be related to 0.5% n-dodecane could maintain dissolved oxygen concentration > 32% of air concentration compared with 23.8% in ε-PL production phase, and the production of a main by-product, poly-L-diaminopropionic acid, fell by 31%. These results indicated that the dissolved oxygen level in the broth was improved by adding n-dodecane, which can inhibit the by-product production and improve the biosynthesis of ε-PL.

Propionic acid fermentation by Propionibacterium freudenreichii CCTCC M207015 with a fibrous-bed bioreactor / 生物工程学报

The production of propionic acid by Propionibacterium freudenreichii CCTCC M207015 was investigated in a Fibrous-bed bioreactor (FBB). The FBB was constructed by packing spiral cotton fibrous and immobilized into a bioreactor. By applying this bioreactor to propionic acid fermentation, the propionic acid yield had a significant improvement and reached 20.41 g/L, compared with the cell-free culture of 14.58 g/L (40 g/L of glucose). At the same time, the glucose exhausting time decreased from 120 h to 60 h. Batch fermentations at various glucose concentrations were carried out with FBB. Based on the analysis of the time course of production, fed-batch fermentation was also applied to produce propionic acid with FBB, the maximal propionic acid yield reached 45.91 g/L, and the proportion of propionic acid to total acids was about 72.31%.

Expression of HOXA 11 in human endometrial hyperplasia and adenocarcinoma / 基础医学与临床

Objective To examine HOXA11,ER and PR expression in human endometrial hyperplasia and adenocarcinoma.Methods The immunohistochemistry was applied to analyze the level of HOXA11、ER and PR expression in the proliferative endometria,simple hyperplasia and endometrial adenocarcinoma.Results The expression level of HOXA11 in the endometrial hyperplasia and adenocarcinoma was significantly higher than that of proliferative endometrium(P