ABSTRACT
ObjectiveToexplorethedynamicchangesofoxidativestressandcytokinesinMongoliangerbilswith nonalcoholic fatty liver disease ( NAFLD) and their significance.Methods Forty-eight healthy male gerbils were randomly divided into normal group and model group , 24 in each group .Gerbils of the model group were fed with high fat diet while those of the normal group with normal diet .Eight gerbils in each group were killed at the end of 4 w, 8 w and 16 w, respectively .MDA content and SOD , GSH-PX and T-AOC activity in the liver tissue were detected by chemical method, and serum TNF-α, INF-γand IL-10 levels were determined using liquid suspension chip .Results With the development of NAFLD , MDA content in liver increased gradually , and the MDA contents were all significantly higher than those of the normal group ( P<0.01 ); T-AOC level slightly increased , and then decreased , the levels at 4 w and 16 w were markedly decreased compared with those of the normal group (P<0.05);SOD level was significantly increased and then markedly reduced, the level of the model group at 4 w was significantly increased (P<0.05), while that at 8 w and 16 w were significantly decreased (P<0.05, P<0.01).The level of GSH-PX was decreased gradually , the levels at 8 w and 16 w were significantly lower than those of the normal group (P<0.05).With the progression of NAFLD,serum TNF-αand IFN-γwere increased gradually , while the level of IL-10 decreased gradually , and the levels at 8 w and 16 w were significantly lower than those of the normal group ( P <0.05, P <0.01).Conclusions The oxidative stress-related indicators and inflammatory cytokines in the gerbil NAFLD models induced by high fat diet are significantly changed as simple fatty liver develops into steatohepatitis , liver fibrosis and cirrhosis , and participate in the development and progression of NAFLD .
ABSTRACT
The effects of RHPS & APS on immune function in normal mice and immunosuppressed mice were studied. The results showed that RHPS and APS enhanced not only the phagocytosis of peritoneal mac-rophages in normal mice, but also antagonized completely the immun- Osuppresslve effect of prednisoione. RHPS and APS increased both the lymphocyte transformation rate induced by PHA and ANAE+ rate in normal mice, and antagonized also the suppressive effects of GY or PDS. No significant influence was found on the proliferation of rabbit lymphocyter treated with PHA in vitro. It seems that no obvious difference of effect was found on immune function between RHPS and APS.
ABSTRACT
The effects of radix hedysari polysaccharide (RHPS)and Astragli polysac-charide(APS)on immune function was studied in normal mice and in themice whose immune function had been suppressed by the use ofcyclophosphamide(CY)or prednisolone(PDS).RHPS or APS 500mg,1000mg/kg ip qd?6d to mice,could increase spleen weight(p