ABSTRACT
Objective To observe the serum vascular endothelial growth factor (VEGF),apelin and heme oxygenase-1 (HO-1) levels in patients with type 2 diabetes mellitus (T2DM) and to explore their their relationship with diabetic retinopathy (DR).Methods A total of 208 patients with T2DM and 50 healthy subjects (control group) from the Central Hospital of Western Hainan during January 2014 and December 2017 were selected in this study.Vision,slit lamp microscope,indirect ophthalmoscope and FFA examinations were performed on all the subjects.According to the results of the examinations combined with the DR clinical staging criteria,the patients were divided into non-DR (NDR) group,non-proliferative DR (NPDR) group,and proliferative DR (PDR) group,with 72,76 and 60 patients in each,respectively.The clinical data of each group were recorded,and the levels of fasting blood glucose (FPG),HbA1c,total cholesterol (TC),three acylglycerol (TG),high density lipoprotein (HDL-C),low density lipoprotein (LDL-C),VEGF,apelin and HO-1 were detected in each group.The receiver operating characteristic curve (ROC) were used to analyze the value of VEGF,apelin and HO-1 in predicting the occurrence of PDR.Correlation analysis of serum VEGF,Apelin and HO-1 with clinical parameters in PDR patients by Pearson correlation analysis.Results The level of VEGF (56.82± 10.16 vs 91.74±22.83,140.15±36.40,195.28±42.26 pg/ml)and apelin (2.95±0.53 vs 4.68±0.74,7.25±1.13,10.16± 1.35 ng/ml) in PDR group were significantly higher than those in NPDR,NDR and control groups (F=17.306,21.814;P＜0.05).The level of HO-1 (50.37±10.14 vs 43.58±8.16,30.25t6.28,22.60±4.72 mmol/L) in PDR group was significantly lower than those in NPDR,NDR and control groups (F=15.827,P＜0.05).The ROC curve analysis showed that the best cut-offvalues of serum VEGF,apelin and HO-1 were 162.50 pg/ml,8.30 ng/ml,27.13 mmol/L,and the three combined to predict PDR of AUC (95％CI)was 0.906 (0.849-0.962),and their sensitivity (90.3％) and specificity (83％) were better.The correlation analysis showed that the VEGF,apelin and HO-1 of PDR patients were correlated with the course of diabetes (r=0.382,0.416,-0.36;P＜0.05),FPG (r=0.438,0.460,-0.397;P＜0.05) and HbAlc (r=0.375,0.478,-0.405;P＜0.05),and the serum VEGF were correlated with apelin and HO-1 (r=0.793,-0.594;P＜0.01).Conclusion Elevated serum VEGF and apelin levels and reduced HO-1 levels are associated with the progression of DR,and the three combination helps predict the occurrence of PDR.
ABSTRACT
Objective To investigate the expression and clinical significances of miR-215 and runtrelated protein1 (RUNX1) in retinoblastoma (RB),and to study the regulation effect of miR-215 on RUNX1 in the retinoblastoma cell line PMC-RB.Methods The expressions of miR-215 and RUNX1 in the tumor tissue,non tumor tissues adjacent to cancer,human RB cell line FMC-RB and human normal retinal vascular endothelial cell line ATCC of RB patients were detected by quantitative real-time polymerase chain reaction (qRT-PCR).miR-215 mimics (miR-215-mimic),miR-NC,si-RUNX1 and si-NC were transfected into FMC-RB cell line respectively.Cell proliferation,migration and invasion ability were measured respectively,thus detecting the regulation effect of miR-215 on RUNX1.Results The expression of miR-215 in RB tissues was significantly lower than that in non tumor tissues adjacent to cancer,while the mRNA expression of RUNX1 was higher than that in non tumor tissues adjacent to cancer (P ＜ 0.05).The expression of miR-215 in PMC-RB cells was lower than that in ATCC,while the mRNA expression of RUNX1 was higher than that in ATCC (P ＜0.05).The expression of miR-215 and RUNX1 in RB tumor tissues were closely related to the clinicopathological features of optic nerve infiltration,tumor tissue differentiation and lymph node metastasis (P ＜ 0.05).Cell proliferation,migration and invasion in miR-215-mimic group were significantly lower than those in miR-NC group (P ＜ 0.05).In transfected 3' untranslated region (3 'UTR)-Wt cells,the luciferase activity in miR-215-mimic group was lower than that in miR-NC group (P ＜ 0.05);the expression level of RUNX1 protein in transfected miR-215-mimic cells was lower than that in transfected miR-NC cells (P ＜ 0.05).Cell proliferation,migration and invasion in si-RUNX1 group were all lower than those in si-NC group (P ＜ 0.05).There was a negative correlation between mRNA expression level of miR-215 and RUNX1 in RB tumor tissues.Conclusions During the occurrence and development of RB,the down-regulation of miR-215 expression can promote malignant progression of tumor by targeting RUNX1.miR-215 can be used as a biological markers and therapeutic target for RB diagnosis.