ABSTRACT
The infection by Limnodrilus hoffmeisteri Claparède, 1862 (Oligochaeta: Tubificinae) in humans is relatively uncommon. The present report is to describe an incidental human infection with oligochaetes in the nasal cavity of a Chinese man, a 25-year-old man residing in Zhangjiakou city, Hebei province, China presenting with nose bleed, severe itching, continuous sneezing, and rhinorrhea. A lot of oligochaete worms were found in the nasal discharge of the patient. The detected worms were identified as Limnodrilus hoffmeisteri (Annelida: Oligochaeta) based on morphological and molecular characteristics. This incidental L. hoffmeisteri nasal infection is the first case in China and indicates that oligochaete worms can be encountered in humans.
Subject(s)
Adult , Humans , Asian People , China , Epistaxis , Nasal Cavity , Pruritus , SneezingABSTRACT
Objective To explore the success rate and the risk of establishment of the acute myocardial infarction model between the beagle dogs and the mini-pigs by interventional technique ,further to provid theoretical basis for choose a more suitable animal model .Methods 6 dogs and 6 mini-pigs were anaesthetized ,then underwent the coronary arteriography via femoral artery .After is-chemic preconditioning the coronary balloon was inflated to occlude the middle left anterior descending coronary for 180 minutes . The electrocardiogram was examined throughout the operation and the pathological sections were examined until the animals were executed one week later .Results All beagle dogs survived ,while 1 case of mini-pigs dead(1/6) .There was 1 cases(1/6) of beagle dogs had acute myocardial infarction ,while 5(5/6)cases in mini-pigs .All mini-pigs had malignant arrhythmia(6/6) but never seen in beagle dogs .The time needed for building a model was similar between the two groups ,the difference had no statistical signifi-cance(P>0 .05) .Conclusion The risk of establish myocardial infarction model in mini-pigs is higher than beagle dogs ,but the suc-cess rate is still high ,it might be the better choice .
ABSTRACT
After freeze-drying, the ultrastructure of boar sperms was observed by optical and electron microscopy. The in vitro development ability of the sperm was also examined with intracytoplasmic sperm injection (ICSI). The rate of male pronuclear formation was (68.52%), for cleavage (59.17%) and for blastocyst formation (19.16%) of the trehalose group (0.2 mol/L), significantly higher than those of the 50 mmol/L EDTA group (64.59%, 56.26% and 15.62%) and the control group (35.36%, 52.33% and 8.60%) (P < 0.05). After storage for 60, 120 and 180 d at 4 degrees C, no significant difference in the in vitro development was observed (P > 0.05). The male pronuclear, cleavage and blastocyst formation after ICSI with freeze-dried spermatozoa incubated for 1 h was superior than those incubated for 2 h (P < 0.05). No significant differences in the structures after stored at 4 degrees C or -20 degrees C (P > 0.05) were observed between the trehalose group and EDTA group. The percent of B grade freeze-dried boar spermatozoa in the trehalose group was higher than that of the EDTA group (P < 0.05). Based on the ultrastructure observation, main cryogenic damage in freeze-dried boar spermatozoa was swelling, damage or rupture in the sperm acrosome, neck and tail.
Subject(s)
Animals , Male , Freeze Drying , Semen Preservation , Methods , Sperm Injections, Intracytoplasmic , Spermatozoa , Swine , Trehalose , PharmacologyABSTRACT
AIM To investigate the significance of CGRP and ET in the coronary syndrome and the significance of their changes in the incidence of the acute coronary syndrome.Methods Group with UA group ( n =30), AMI group ( n =28), control group ( n =25) were established.In control group blood sample take on an empty stomach in the morning ,while group with AMI on hospitalization and after one week, group with UA at angina pectoris attack and after remission. The concentration of plasm CGRP and ET were measured .Results The plasm CGRP concentration in group with AMI was higher than that in group with UA and that in control group ,the plasm ET elevated markedly, while the rate of CGRP/ET decreased; Compared with that in the remission period of the acute coronary syndrome ,The plasm CGRP elevated, the plasm ET elevated markedly, while the rate of CGRP/ET decreased.Among defferent acute coronary syndrome ,the plasm CRGP and ET concentrantion was highest in AMI group,higher in group with accelerated UA, high in group with initial attack UA;the rate of CGRP/ET was highest in group with initial UA,higher in group with accelerated UA,high in group with AMI.Conclusions The changes of plasm CGRP and ET concentration are well correlated with the extent of coronary lesion, the level of plasm CGRP and ET and the imbanlance of thier receptor play an important role in the pathogenesis and development of acute coronary syndrome. [