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Objective:To examine the reliability and validity of the Chinese version of individuation test for emerging adults short version (ITEAS) among college students.Methods:A total of 1 778 college students were investigated with the Chinese version of the ITEAS, the separation-individuation test of adolescence subscales, including the separation anxiety subscale and dependency denial subscale, and the Rosenberg self-esteem scale from August to October 2021.Ninety of these college students were retested with the Chinese version of the ITEAS after a 4-week interval.And data were analyzed by SPSS 26.0 and AMOS 21.0.Results:Exploratory factor analysis found that both the revised ITEAS for fathers (ITEAS-F) and ITEAS for mothers (ITEAS-M) had 21 items in five dimensions, including seeking support, connection, invasion, self-reliance and fear of disappointing the parents.Confirmatory factor analysis revealed that the five-factor models for both the ITEAS-F and ITEAS-M scales fit well ( χ2/ df=2.27, CFI=0.92, TLI=0.90, RMSEA=0.06, SRMR=0.07; χ2/ df=2.38, CFI=0.94, TLI=0.93, RMSEA=0.06, SRMR=0.07). The criterion-related validation showed that the dimensions of seeking support, connection, and self-reliance in both the ITEAS-F and ITEAS-M were significantly negatively correlated with separation anxiety and rejection of reliance, and were significantly positively correlated with self-esteem.Moreover, the dimensions of invasion and fear of disappointing the parents were significantly positively correlated with separation anxiety and rejection of reliance, and were significantly negatively correlated with self-esteem.Furthermore, the Cronbach’s α ranged from 0.79 to 0.90 ( P<0.01) and 0.82 to 0.92 ( P<0.01), respectively.And the retest reliability ranged from 0.73 to 0.86 ( P<0.01) and 0.78 to 0.86 ( P<0.01), respectively. Conclusion:The Chinese version of ITEAS meets the requirements for psychometric assessments and is a valid and reliable instrument.
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Objective:To understand counselors' use of social media from the perspective of clients, and find the relevant ethical issues. Methods: The questionnaire of how clients regarding counselors'use of social media was made as evaluation tool. Totally 434 valid questionnaires were received, including 86 males and 348 females, aged (36 ±7) years. Results: Almost 51.6％ of the clients got information of counseling and counselors through social media, and 35.9％ received online counseling through social media. Several ethical issues existed. Counselors didn't make informed consent with clients, which included the confidentiality, security and real identity about online counseling issues. Clients and counselors added WeChat or QQ friends and interacted with each other outside the counseling process. This situation led to multiple relations. Counselors' personal exposure in their social media negatively impressed clients. Counselors had over propaganda or clients'privacy expose during advertising or training through social media. Conclusion: Counselors' using social media for counseling involve many ethical issues. The confidentiality and multiple relations are more complex and subtle due to Internet features. The ethical issue of counselors' self-exposure in personal social media particularly requires being noticed.
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Objective: To understand grief stages, grief reactions and grief pathways of college student after lovelorn, in order to provide basis for psychological services. Methods: Totally 11 college students who were broken-up for at least 1 year were recruited and screened. Qualitative research methods and semi-structured interviews were used in this study. After the interview, the recording was copied out into transcripts and was analyzed. Results: The grief stages after lovelorn could be divided into "strong emotions and behavior", "depression and decreased behavior or transfer notice obsessed with other things", "looking for connection with the break up", and "put feelings, fit and ready to start a new life". There were also some specific grief reaction. During the first grief stage, people with different gender had different reactions, and the one who proposed breakup would feel relaxed and guilty. If the ex-lover began with a new relationship, it would easily lead to emotional reaction again. People with objective reasons for breakup would have expectations for the future. Conclusion: It suggests that college students have obvious grief reactions and grief periods after lovelorn. Gender, subjective or objective factors may lead to specific grief reactions, which should be paid more attention to.
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Objective:To explore the types and characteristics of the grief reactions of adolescents to friends'death repeatedly,so as to provide reference for effective psychological intervention. Methods:Three college students aged nineteen and twenty years who experienced the death of friends at least twice in adolescence participa-ted in the study by means of an open recruitment in a college. They had friends died at the age of 9 and 12. And then it happened again when they were 15 and 17. Data was collected by semi-structured interviews with the three selected,and analyzed by thematic analysis to classify the grief reactions after the loss of their friends. Results:The common grief reactions of a friend's death in the interview are:shocked,regret and angry,denying,disbelieving, fear of the death,feeling the cruelty of death,crying,searching for support,shirking and recognizing the cruelty of death. The special grief reactions in the interview,especially when they experienced the second death of a friend are:caring for the dead loved ones,having a mental shadow over friend's role,reducing social activities,seeking stimulating and venting,revolting against the school,violence,and working harder. Conclusion:The grief reactions of adolescents to friends'death repeatedly are diversity. It presents the characteristics of the psychological develop-ment of adolescence. And the adolescents need to be given special attention and appropriate psychological interven-tion.
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This paper is to introduce our experiences in treating 2 batches of 13 burn victims transferred from remote areas on postburn days 3 and 4. Methods Thirteen burn victims of 2 mass casualties were transferred to our burns institute from remote areas on postburn days 3 and 4 on June 27, 2001 and June 2, 2002, respectively. There were 4 males and 9 females, age ranged from 20 to 43 years, with a mean age of 31.1±6.2 years. The mean total burn area was 74.3%±24.7% TBSA (range, 25% to 97%). Among them, 10 patients suffered from serious burn with mean total burn area involving 86.0%±11.5% TBSA (range, 60% to 97%), and mean full-thickness burn of 63.9%±26.3% TBSA. Four patients also manifested signs of severe inhalation injury, and 6 patients with moderate inhalation injury. In three patients with mean total burn area covering 35.5%±10.0% TBSA (range, 25% to 45%), with mean full-thickness burn of 15.3%±5.0%, were al having moderate inhalation injuries. Among these 13 patients, 3 were having high body temperature (39℃), while 3 manifested hypothermia. The heart rate was 140-160/min, and respiratory rate 26 to 32/min in 6 patients. Abdominal distension or loss of bowel sound were found in 4 patients. Low white cell and platelet count were found in some patients. In 13 cases, liver function, renal function, myocardiac enzyme, and coagulation function were abnormal. Results Among 13 burn victims, one patient died of myocarditis on postburn day 29, and another one died of hepatic failure (history of chronic hepatitis B) on postburn day 45 with only 2% TBSA of burn wound remained open. Conclusion Burns victims occurred in mass casualties who were transferred from remote areas to our Burns Institute were all in critical condition, usually with multiple complications, demanding most meticulous care. Our strategies in this regard consisted of dispatch of experienced surgeons and nurses to the referring hospitals and the airport to receive the patients to offer appropriate care to them during the journey,organization of the medical staff so that each of them was ordained specific function, thus conditions of the patients were evaluated immediately and appropriate treatment started expeditiously for those lethal complications on arrival. Timely and exact comprehensive treatments were prerequisite to save the patients’ life. Adequate metabolic support should be emphasized, and coagulant of anticoagulant treatment should be carried out when indicated.
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Aim To pick out the siRNA which could most effectively inhibit the expression of brain-derived neurotrophic factor( BDNF) in microglial cells,to detect the cytotoxicity of the transfection complex,and to ob-serve the change of OX-42 expression,the microglial marker,after BDNF siRNA treatment. Methods Four siRNAs were chemically synthesized: three of them were used to inhibit BDNF expression in microglial cells,the rest was fluorescence-labeled mismatch siRNA as a negative control. They were all transfected into microglial cells,respectively. BDNF mRNA was detected 24 h after transfection by Real-Time PCR and itsprotein expression was observed done by Western blot 48 h later. The Sulforhodamine B( SRB) assay was used to investigate the drug-induced cytotoxicity. Co-expres-sion pattern of BDNF and OX-42 was determined by double-labeling immunofluorescence. Results ① The BDNF siRNA1588 was the most effective siRNA,compared with the vehicle or mismatch siRNA-treated group( P
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<p><b>OBJECTIVE</b>the main purpose of this study is to develop an assay for detection of skin viability in situ.</p><p><b>METHODS</b>Two nucleic acid dyes, SYTO 13 and ethidium bromide (EB), are used to assess the viability of skin tissue. The viability of cells can be determined by different colors under fluorescence microscope. In this study, the recovery of skin restored at 4 degrees C for different periods is measured by SYTO/EB dyes and a type of indirect assessment, WST-1. Then both results are compared.</p><p><b>RESULTS</b>The results measured by two methods showed that the viability of skin tissue was nearly 30% after two weeks storage. At any point of experiment, the data from two methods have not obvious variance.</p><p><b>CONCLUSION</b>The assessment of skin viability by SYTO/EB is also quick, sensitive and convenient. At the same time the viability of any type of cells can be observed in situ. So it can be considered as an available method for detection of skin viability.</p>
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Animals , Male , Rats , Cell Survival , Physiology , Cryopreservation , Ethidium , Models, Animal , Skin , Cell Biology , Metabolism , Skin Physiological Phenomena , Tissue Preservation , MethodsABSTRACT
The effect of antiepilepsirine, a derivative of piperine, on the rat amygdala kindled seizures was studied. An average of daily consecutive 15 stimulations was needed to reach the first stage 5 seizures, at which the rats showed behavioral convulsions and electroencephalogra-phic abnormalities. Antiepilepsirine ip 2 h before stimulation at the doses that did not show obvious sedation suppressed the stage 5 seizures with the ED50 of 84.7mg/kg and exhibited insignificant shortening of afterdischarge duration. Sodium valproate effectively protected against stage 5 seizures and shortened the afterdischarge duration. Present data suggest the chronic antiepileptic property of antiepilepsirine in rat amygdala kindling model.