ABSTRACT
This study was aimed to detect the heavy metal residues of medicinal leeches, in order to understand the current market circulation situation of medicinal leeches, and to analyze possible factors which may cause heavy metal pollution, and to provide references for the standardized safe cultivation of medicinal leeches. Inductively coupled plasma mass spectrometry (ICP-MS) technique was applied to detect heavy metal residues in medicinal leeches. The results showed that medicinal leech samples tested for heavy metal content severely exceeded the standard, which caused a great threat to the safety of medication. It was conclude that more attentions should be paid on factors for causing heavy metal accumulation within medicinal leeches during the breeding process. The related department should also list safety limits explicitly and separately for animal drugs such as medicinal leech during the development of quality standards of Chinese materia medica.
ABSTRACT
Objective To investigate the effects of a highly selective dopamine D4 receptor (ABT-724)on behaviors in Spontaneously hypertensive rats (SHR),a rat model of attention-deficit hyperactivity disorder (ADHD).Methods After intervention of methylphenidate(5mg/kg) and three different doses of ABT-724(0.04mg/kg,0.16mg/kg,0.64mg/kg),behaviors of SHR were verified by open-field test,Morris water maze and Làt maze.Results Number of square crossing after intervention of methylphenidate and ABT-724 in SHR( 70.67 ± 8.59,76.50 ± 10.75,79.17 ± 10.44,65.67 ± 20.62) was less than the saline control group( 130.33 ± 1 1.40) (P＜0.05).During Morris water maze,SHRs(52.50 ± 4.04,52.17 ± 2.99,61 ± 8.15,53.83 ± 9.87 ) after intervention of both had a better spatial memory ability than control group(38.83 ±7.17) (P＜0.05).In Làt maze,number of rearing after intervention of both in SHRs(57.17 ± 5.67,60.83 ± 8.28,55.17 ± 9.45,65.33 ± 9.50 ) was less than saline control group(78.00 ± 13.84) (P＜0.05).Conclusion ABT-724 could improve behaviors of spontaneous locomotor activity,cognitive ability,non-selective attention in SHR.