ABSTRACT
ObjectiveTo screen out differentially expressed genes (DEGs) and related candidate therapeutic drugs for acute pancreatitis (AP) using the bioinformatics method. MethodsHigh-throughput microarray datasets (GSE109227 and GSE65146) associated with AP in mice were downloaded from gene expression omnibus, and GEO2R was used to screen out DEGs. Database for Annotation, Visualization and Integrated Discovery was used to perform gene ontology and pathway enrichment analysis. Protein-protein interaction (PPI) was established in String database and visualized by Cytoscape, and then subnetwork modules and hub genes were screened out. The microRNAs associated with the hub genes were predicted and candidate drugs were screened out using Comparative Toxicogenomics Database (CTD). ResultsA total of 130 upregulated and 16 downregulated DEGs were screened out in the high-throughput microarray datasets GSE109227 and GSE65146. DEGs were mainly involved in the biological processes such as inflammatory response, neutrophil chemotaxis, tumor necrosis factor-mediated cellular response, and positive regulation of gene expression, and they were also involved in the signaling pathways of extracellular matrix-receptor interaction, regulation of actin cytoskeleton, leukocyte transendothelial migration, and focal adhesion. A total of 12 hub genes and 6 subnetwork modules were screened out in the PPI network. The microRNAs including miR-199a-5p and miR-1-3p might regulate the post-transcriptional regulation of key genes. Genistein, resveratrol, and quercetin which were screened out from CTD database could reduce the expression of key genes. ConclusionRelated genes screened out by the bioinformatics method may play an important role in the development of AP and can be used as the basis for drug screening.
ABSTRACT
Objective To investigate the effect of traditional Chinese medicine retention-enema combined with tamsulsosin hydrochloride capsules in treating chronic abacterial prostatitis/chronic pelvic pain syndrome. Methods Two hundred patients of CABP/CPPS from Affiliated Hospital of Gansu University of TCM during September 2007-October 2012 were randomly divided into control group and observation group, 100 cases in each group. The control group received the treatment of tamsulsosin hydrochloride capsules, 0.2 mg/d, qn, and the observation group received the treatment of traditional Chinese medicine retention-enema additionally. The course was 4 weeks. The clinical effects of the two groups were observed and compared. The indexes including the scores of NIH-CPSI, leukocyte count and lecithin corpuscles in prostatic fluid, urinary flow rate were evaluated. Results The total effective rate was 100%(100/100) in observation group, and 88% (88/100) in control group. There was significant difference between the two groups (P0.05). Conclusion Traditional Chinese medicine retention-enema combined with tamsulsosin hydrochloride capsules can significantly improve the clinical effect of CABP/CPPS.
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OBJECTIVE To investigate the causes and preventive measures of postoperative pneumonia due to cervical part and upper thoracic part esophageal carcinoma surgery. METHODS Fifty seven cases with cervical part or upper thoracic part esophageal carcinoma from 2001 to 2005 were studied restropectively. RESULTS One patient died of aspiration pneumonia,one died of anastomotic leakage,and three died of adult respiratory distress syndrome(ARDS).The tracheotomy and mechanical ventilation were applied.Pneumonia was confirmed among sixteen patients at different degree. CONCLUSIONS Many causes contribute to postoperative pneumonia on patients with cervical part or thoracic part esophageal carcinoma,and the lesion of recurrent nerve is an important reason.Protection of recurrent nerve may decrease postoperative pneumonia.
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Objective To study the correlation between spiral CT(SCT)feature of non-small cell lung cancer(NSCLC)and the levels of soluble Fas(sFas)and FasL(sFasL)in serum.Methods 32 cases of NSCLC proved pathologically and examined with SCT were analyzed retrospectively.Serum level of sFas and sFasL were determined by double antibody sandwich Enzyme linked immunosorbenl assays(ELISA).The SCT features were compared with level of sFas and sFasL.Results No significance was found between serum levels of sFas and sFasL and tumor type and size(P
ABSTRACT
ObjectiveTo screen out differentially expressed genes (DEGs) and related candidate therapeutic drugs for acute pancreatitis (AP) using the bioinformatics method. MethodsHigh-throughput microarray datasets (GSE109227 and GSE65146) associated with AP in mice were downloaded from gene expression omnibus, and GEO2R was used to screen out DEGs. Database for Annotation, Visualization and Integrated Discovery was used to perform gene ontology and pathway enrichment analysis. Protein-protein interaction (PPI) was established in String database and visualized by Cytoscape, and then subnetwork modules and hub genes were screened out. The microRNAs associated with the hub genes were predicted and candidate drugs were screened out using Comparative Toxicogenomics Database (CTD). ResultsA total of 130 upregulated and 16 downregulated DEGs were screened out in the high-throughput microarray datasets GSE109227 and GSE65146. DEGs were mainly involved in the biological processes such as inflammatory response, neutrophil chemotaxis, tumor necrosis factor-mediated cellular response, and positive regulation of gene expression, and they were also involved in the signaling pathways of extracellular matrix-receptor interaction, regulation of actin cytoskeleton, leukocyte transendothelial migration, and focal adhesion. A total of 12 hub genes and 6 subnetwork modules were screened out in the PPI network. The microRNAs including miR-199a-5p and miR-1-3p might regulate the post-transcriptional regulation of key genes. Genistein, resveratrol, and quercetin which were screened out from CTD database could reduce the expression of key genes. ConclusionRelated genes screened out by the bioinformatics method may play an important role in the development of AP and can be used as the basis for drug screening.