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Objective To explore the clinical analysis of 65 cases of elderly patients with gastric carcinoma. Methods The clinical data of 65 cases of elderly patients with gastric carcinoma from October 2002 to September 2007 were analyzed retrospectively. Results 34 eases took place a variety of complications in this group of 26 cases of patients,and they are 5 cases of incision dehiscence,2 cases of anastomotie leakage, 1 cases of duodenal stump leakage,1 cases of gastric dysfunction,ll cases of heart failure,14 cases of lung infection,3 cases of urinary tractin-fection, two case of intra-abdominal infection, two cases of intestinal obstruction, 1 case of postoperative died. Conclu-sion As early as mid-term elderly gastric cancer should be perfrmed radical resection,at the same time,prevent and treat complications actively.
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@#Objective To analyze the outcome of total knee arthroplasty with retaining patellar in osteoarthritis.Methods 45 knee joints in osteoarthritis were performed operatively with posterior stabilized knee arthroplasty between January 2005 and June 2008. The series include 7 sides(males) and 38 sides(females). The mean age of patients was 65.7 years ranging from 52 to 79 years, and the data were analyzed by The Hospital for Special Surgery(HSS) score system. Results The follow-up period was averagely 25 months ranging from 8 to 51 months. According to HSS score system, 28 sides were rated as excellent（62.2%）, 12 sides as good（26.7%）, 4 fair（8.9%）and 1 poor（2.2%）, and the excellent-good rate was 88.9%. Conclusion Posterior stabilized knee arthroplasty with retaining patellar represents a simple and valuable treatment methodology for the most osteoarthritis of the knee joint.
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Objective To investigate the effect of thymosin α1 on immun ologic function and cytokines in the abdominal sepsis patients.and naddiscuss the therapeutic function of thymoin α1 in sepsis.Methods Thirty-eight sepsis patients admitted between May 2006 and May 2007 in the Hospital of Weifang Medical University.Patients were divided into the treatment group(20 cases)and control group(18 cases)randomly.Thymosin α1 was used in the treatment group on the basis of infection controlled.The concentration of cytokines(TNF-α,IL-6,IL-10)and the percent age of CD3,CD4,NK cell were observed.Results The concentrations of TNF-α and IL-6 were increased significantly in bothgoups.But it was more prominently in the control group.The concentration of IL-10 and the percent age of CD3,CD4,NK cell increased more significantly in thymosinod treatment group than those in the control group.Conclusions Thymosincd canmodulate the release of TNF-α,IL-6,IL-10 and mitigate the inflammatory reaction,which may improve the immunologic function.
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Objective To study the hypoglycemic effect of polysaccharide from stigma maydis and to explore its therapeutic mechanism preliminarily.Method The diabetes mice models were induced by tetraoxypyrimidine.The concentration of blood sugar was determined by glucose oxidase test.The influence of the stigma maydis polysaccharide on glycometabolism and its repair effect on damaged glycometabolism organs of diabetic mice were observed respectively by content determination of glycogen and by the measurement of activities of relative enzymes(superoxyde dismutase and glutathione peroxidase).Results The stigma maydis polysaccharide could decrease the blood sugar,promote the synthesis of glycogen and repair the damaged glycometabolism organs of diabetic mice.Conclusion The stigma maydis polysaccharide exhibits a good therapeutic effect on diabetic mice,and has a good development prospect.
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Objective To investigate the isolation,incubation and identification of murine yolk sac mesenchymal stem cells(YS-MSCs). Methods Murine yolk sacs were harvested on the 10th day of postcoitus microisolation.Yolk sac cells were obtained after the yolk sacs were digested by type Ⅰ collagenase of 0.1% for 1 hour.Adherent cells were cultured in DMEM containing 10% FBS and 1% SP,and passaged when they became subconfluent.The ultrastructure of YS-MSCs was observed under transmission electron microscope;the surface markers of YS-MSCs were detected by flow cytometry;the alkaline phosphatase(AKP) expression of YS-MSCs was tested;and cytochemical changes of YS-MSCs were observed with cytochemical detection.Adipogenic differentiation of YS-MSCs was induced by 1?mol/L dexamethasone and 10mg/L insulin,and oil red O was used for fat staining. Results YS-MSCs were obtained in vitro,and most of them were of spindle shape;under transmission electron microscope,there were microvilli on the surface of YS-MSCs,there were abundant mitochondria,rough endoplasmic reticulum and golgi complex in the endochylema.The cell nuclei of YS-MSCs were big and the shape of cell nucleus was irregular;the analysis of flow cytometry suggested that the primary and first generation of YS-MSCs were positive for CD44,CD105,and the expression of CD34 was small;Cytochemistry showed that YS-MSCs were positive for glycogen,and negative for SB and AKP;Adipogenic differentiation of YS-MSCs was induced by 1?mol/L dexamethasone and 10mg/L insulin;accumulation of lipidrich vacuoles appeared in the cells,positive for oil red O staining.Conclusion The biological characteristics of murine yolk sac mesenchymal stem cells are similar to,and more primitive than,those of adult mesenchymal stem cells.It suggests that YS-MSCs may be used as an ideal resource of seed cells of tissue engineer.