ABSTRACT
OBJECTIVE:To establish quality standard for Jingbi mixture. METHODS:TLC was adopted to identify the Notop-terygii Rhizoma et Radix,Aucklandia lappa and Pueraria lobata and determine the relative density and pH according to pharmaco-poeia method. HPLC was adopted to determine the content of puerarin. Column was XTerra? RP18 with mobile phase of acetoni-trile-0.05% phosphoric(10:90,V/V)at flow rate of 1.0 ml/min,detection wavelength was 250 nm,column temperature was 25 ℃and volume injection was 10 μl. RESULTS:TLC of Notopterygii Rhizoma et Radix,A. lappa and P. lobata showed clear spots and good separation. The relative density was 1.08,and pH was 4.5. The linear range of puerarin was 2.079-33.26 μg/ml(r=0.999 8);RSDs of precision,reproducibility and stability tests were lower than 1.0%;recovery was 99.50%-100.50%(RSD=0.42%,n=6). CONCLUSION:The method is simple and good reproducibility,and can be used for the quality control of Jingbi mixture.
ABSTRACT
Borneol in Yuqingan liquid was determined quantitatively by GC. The sample was first extracted by ethyl acetate and component determined by two types of column packed with 10%OV-17 and 10% PEG-20M/chromosorb WHP respectively. N-tetradecane was used as an internal standard. The method isparticularly advantageous for the determination of Borneol