Effect of iodine excess on morphology of mouse thyroid follicle and pancreatic acinar cells / 中华地方病学杂志

Objective To explore the effect of different levels of iodine excess on morphological changes of mouse thyroid follicle and pancreatic acinar cells.Methods Sixty female mice (BALB/c) were selected and their body weight were 18-22 g.The mice were divided into 6 groups according to body weight via the random number table method,10 mice in each group.Potassium iodate was added to drinking water in exposure groups with iodine contents of 300,600,1 200,2 400,and 4 800 μg/L,while normal group (control) was given normal levels of iodine (5 μg/L) tap water.After feeding for one month,the thyroid and pancreas of the mice were harvested,and the morphology of thyroid follicle and pancreatic acinar cells were observed through light microscope and ultrastructural changes of pancreas were observed through electron microscope.Results After one month of feeding,mice in the high iodine drinking water groups,starting from the 1 200 μg/L group,thyroid follicular cavity gradually enlarged and cells became flat;swollen and vacuolar-like deformation were observed in the mouse pancreas acinar cells under light microscope.Under the electron microscope,the ultrastructure of pancreatic acinar cells changed significantly starting from the 600 μg/L group,the number of zymogens decreased,organelle degeneration and necrosis,and endoplasmic reticulum expanded.Conclusion Iodine excess can cause damage to pancreatic acinar cells in mice.

Whole body vibration training improves limb motor dysfunction in stroke patients:lack of evidence / 中国组织工程研究

BACKGROUND:Whole body vibration training, a recently developed method of neuromuscular training, is a useful method to improve muscle strength and postural control in the elderly. Recently, researchers attempt to explore whether whole body vibration training can reduce motor dysfunction for stroke patients. OBJECTIVE:To summarize clinical progress in whole body vibration treatment on stroke patients with limb motor dysfunction, including muscle strength of the affected limbs, muscle tension, balance and gait. METHODPubMed, EBSCO, Medline database were searched for articles relate to whole body vibration training intervention for stroke patients with motor dysfunction published from January 2002 to June 2014. Final y, 34 articles were included in result analysis. RESULTS AND CONCLUSION:Whole body vibration training is feasible and safe for stroke patients has the feasibility and safety. For therapeutic efficacy, we focus on the muscle strength, muscle tone, balance, posture control ability, but there is no sufficient evidence to support that the whole body vibration training can improve the motor dysfunction. There is also no recognized standard on specific intervention protocols, such as vibration type, treatment frequency, treatment amplitude, treatment time. Many researchers aim to observe the clinical curative effect at present, but rarely explore the intervention mechanism of the whole body vibration. Further large-sample, multi-center randomized control ed experiments are required to test the validity.

Expression of heat shock protein 27 in in vitro cultured human lens epithelial cells / 中国组织工程研究

BACKGROUND: Special anatomical location makes eye lens expose to stressful situation in a long term. Whether the environmental stress can up-regulate the expression of heat shock proteins in human lens epithelial cells? Whether the synthesis increase occurs in the level of trenscdption or translation, remains unclear.OBJECTIVE: To observe the expression and location of heat shock protein 27 (HSP27) in human lens epithelial cells under the conditions of high temperature and oxidative stress, and to investigate the pathogenesis of the cataract.METHODS: Human lens epithelial cells cultured in vitro were exposed to heat (45 ℃) and oxidative stress (50 mmol/L H_2O_2) for 30 minutes, respectively, then allowed to recover normal conditions. At different intervals (0, 2, 4, 6, 16, 24 hours),immunocytochemistry and reverse transcription polymerase chain reaction were used to determine the expression and localization of HSP27.RESULTS AND CONCLUSION: HSP27 was shown to express in both physiological and stressful conditions. The expressions of HSP27 mRNA and protein ware remarkably increased at 2 hours following heat and oxidative stress, and reached the peak at 6 hours. HSP27 could maintain a high level for 16 hours. The stress-induced HSP27 protein positive particles transferred from the cytoplasm to the nucleus, and gradually shift back to the cytoplasm along time. It is proved that HSP27 exists in lens epithelial cells and can be increased after stress. The data suggested it may play an important protective role in lens epithelial cells in respond to cellular stress.