ABSTRACT
OBJECTIVE:To observe the effect of Guben yangxue oral liquid on cyclophosphamide (CTX)-induced suppression of the immune and hematopoietic system in mice. METHODS:Immunosuppressive model mice were induced by intraperitoneal injection of CTX 50 mg?kg-1. Different dose groups were Guben yangxue oral liquid at the same time with Zhenqi fuzheng capsules as control. Several index such as peripheral blood cells, the weight of immune organ, serum hemolysin were determined. RESULTS:The levels of white blood cells (WBC,87.3、26.3、7.96 g?kg-1), lymphocytes (L,87.3 g?kg-1), neutrophils (N,87.3、26.3、7.97 g?kg-1), hemoglobin (Hb,87.3、26.3 g?kg-1), red blood cell (RBC,87.3、26.3 g?kg-1), platelets (PLT,87.3、26.3 g?kg-1), sp- leen index(87.3,26.3 g?kg-1), thymus index(87.3 g?kg-1), serum hemolysin (87.3、26.3、7.96 g?kg-1)in CTX-induced immunosuppressive mice were increased after administrated of Guben yangxue oral liquid. CONCLUSION:Guben yangxue oral liquid can enhance the non-specific immunity and humoral immune function of CTX-induced immunosuppressive mice, and it can improve CTX-induced hematopoietic dysfunction significantly.
ABSTRACT
OBJECTIVE:To establish the quality criteria of Guben yangxue oral liquid. METHODS:TLC was employed to identify prepared Ligustrum lucidum,Cornus officinalis,Radix Astragali,Radix Rehmanniae Praeparata,and Angelica sinensis,and the content of polysaccharide was determined by ultraviolet spectrophotometry. RESULTS:The TLC spots were clear,well-separated and specific yet without interference from negative control. The linear range of polysaccharide was 0.020 6～0.072 1 mg?mL-1(r=0.999 2) with an average recovery of 98.64%(RSD=0.56%,n=9). CONCLUSION:The established quality criteria is suitable for the quality control of Guben yangxue oral liquid.