ABSTRACT
Accumulating studies have demonstrated that non-coding RNAs (ncRNAs), functioning as important regulators of transcription and translation, are involved in the establishment and maintenance of pregnancy, especially the maternal immune adaptation process. The endometrial stromal cells (ESCs), trophoblast cells, and decidua immune cells that reside at the maternal-fetal interface are thought to play significant roles in normal pregnancy and pregnancy-associated diseases. Here, we reviewed the up-to-date evidence on how microRNA, long non-coding RNA, and circular RNA regulate ESCs, trophoblast cells, and immune cells and discussed the potential applications of these ncRNAs as diagnostic and therapeutic markers in pregnancy complications.
Subject(s)
Pregnancy , Female , Humans , MicroRNAs/genetics , RNA, Long Noncoding/genetics , RNA, Circular/genetics , Trophoblasts , Pregnancy Complications/geneticsABSTRACT
Objective:To study the effects of resveratrol on the expressions of Caspase-3 and Bcl-2 in the retina tissue of the rats with retinal ischemia-reperfusion injury(RIRI),and to investigate the therapeutic effect of resveratrol on the RIRI and its mechanism.Methods:A total of 90 SD rats were randomly divided into sham operation group,model group and treatment group,30 rats in each group.The RIRI models were established by pressing the anterior chamber of the rats.The rats in model and treatment groups received ischemia-reperfusion for 1,6,12,24,and 48 h;the rats in treatment group were treated with micro-syringe intravitreal injection of 0.5 nmol·L-1of resveratrol 5 μL.The retinal tissue structure was observed by inverted microscope.The expression levels of Caspase-3 and Bcl-2 in retina tissue were detected by immunohistochemistry and Western blotting methods.Results:The retinal tissue edema of the rats in model group was found with vacuolar degeneration of the ganglion cells,and the arrangement of the cell layer was loose;the number of retinal ganglion cells was decreased,the boundary was blurred,and the nerve fiber layer thinned obviously.The degree of retinal tissue structure,the degree of injury and the degeneration of ganglion cells in treatment group were lighter than those in model group.The results of immunohistochemistry showed that the expressions of Caspase-3 and Bcl-2 in the retina tissue of the rats in treatment group were significantly increased compared with model group.The results of Western blotting method showed that the expression levels of Bcl-2 protein in the retina tissue of the rats in treatment group at different time points were increased compared with model group;and there were significant differences at 24 and 48 h(P<0.05);the expression levels of Caspase-3 protein in treatment group at different time points were lower than those in model group(P<0.05).Conclusion:Resveratrol can improve the retinal tissue structure of the RIRI rats,and its mechanism may be related to decreasing the expression level of Caspase-3 and increasing the expression level of Bcl-2 in the retinal tissue.
ABSTRACT
BACKGROUND: The reason for the short of cornea donator is the olden donating conception or the limit knowledge of cornea transplantation remains poorly understood. OBJECTIVE: To explore the awareness level of the medical staff to corneal transplantation and corneal donation. METHODS: The study consisted of 820 medical staff from different departments of 5 hospitals, including doctor, nurse, technician, and administrator. Awareness level was evaluated with an interviewer-study. RESULTS AND CONCLUSION: Totally 805 interviewer-studies were effect. The correct answer fill rate in department of ophthalmology was higher than other departments. Different measures should be taken to teach the medical staff to improve the awareness level of the transplantation and corneal donation.
ABSTRACT
BACKGROUND: Capsule opacification is a common complication following implantation of intraocular lenses for a long period. Scholars are looking for an ideal intraocular lens so as to reduce the incidence of capsule opacification. OBJECTIVE: To study the effects of three intraocular optic materials with sharp optic edge on anterior and posterior capsule opacification as well as capsulorrhexis contraction. DESIGN, TIME AND SETTING: A randomized controlled study was performed at the Department of Ophthalmology, Affiliated Hospital of Chengde Medical College from May 2005 to December 2006. PARTICIPANTS: A total of 135 patients (148 eyes) with age-related cataract, including 73 males (80 eyes) and 62 females (68 eyes) and aging 52-81 years with the mean age of (71.44 ?6.83) years, were collected in this study. METHODS: All patients were performed with phacoemulsification combining with implantation of foldable intraocular lenses which were characterized by sharp optic edges. Thereafter, they were randomly divided into three groups: lyophobic material group (n=43, 49 eyes), who were implanted with lyophobic acrylic acid intraocular lens; hydrophilic material group (n=42, 46 eyes), who were implanted with hydrophilic acrylic acid intraocular lens; lyophobic/hydrophilic material group (n=50, 53 eyes), who were implanted with lyophobic/hydrophilic acrylic acid intraocular lens. MAIN OUTCOME MEASURES: Anterior and posterior capsule opacification as well as capculorhexis contraction were quantitatively evaluated 1 year postoperatively. RESULTS: At one year postoperatively, incidences of posterior capsule opacification were 8.3% in the lyophobic material group, 26.7% in the hydrophilic material group, and 15.3% in the lyophobic/hydrophilic material group, respectively, and there was significant difference (P 0.05). CONCLUSION: The optic intraocular lens of sharp optic edge does not have any effects on anterior capsule opacification or capsulorrhexis contraction, but has effects on formation of posterior capsule opacification; in particular, lyophobic acrylic acid can reduce the incidence to posterior capsule opacification.