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Objective To investigate zinc finger of the cerebellum 4(ZIC4) expression in endometrial cancer tissue and its relationship with clinicopathological parameters. Methods Immunohistochemistry was used to detect ZIC4 expression in endometrial paraffin specimens. Results The positivity rate of ZIC4 expression in atypical hyperplasia and endometrial carcinoma was higher than that in normal endometrial tissue. ZIC4 expression was correlated with the clinical stage,differentiation,depth of uterine invasion,and lymph node metastasis of endometrial cancer. Survival analysis revealed that patients with endometrial cancer who had low ZIC4 expression levels had better prognosis. Conclusion The ZIC4 expression level in endometrial carcinoma is significantly increased;thus,ZIC4 expression level can be used to monitor the prognosis of patients with endometrial cancer.
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Objective To investigate the expression and significance of miR?135a?5p in endometrial carcinoma and to correlate expression levels with clinicopathological features. Methods Fifty five endometrial carcinoma samples and thirty normal endometrial tissue samples were surgically obtained,and the expression of miR?135a?5p was quantitated by real?time polymerase chain reaction. The relationship between the expression level of miR?135a?5p and clinicopathological data of individual patients was analyzed. Results The expression of miR?135a?5p was lower in the endo?metrial carcinoma group than in the normal endometrial group(P=0.021),and correlated significantly with the histologic grade of the endometrial neoplasm(P=0.008). Conclusion The expression of miR?135a?5p is lower in endometrial carcinoma than in normal endometrial tissue. In en?dometrial carcinoma,the miR?135a?5p expression status has a statistically significant relationship with the histological characteristics of the tumor.
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Objective To investigate the expression of advanced glycation end products(AGEs)in a rat model of polycystic ovarian syndrome (PCOS)induced by dehydroepiandrosterone. Methods The PCOS rat model was established using dehydroepiandrosterone . After confirming that the model rats were set up successfully,serum AGEs concentrations were measured using competitive ELISA and the expression of AGEs in the ovarian tissues of these rats was detected using immunohistochemistry. Results The serum AGEs level in the experimental group were signifi?cantly higher than that in the control group(P<0.05). Furthermore,AGEs expression were significantly higher in the experimental group than in the control group(P<0.01). Conclusion AGEs are highly expressed in the rat PCOS model,signifying their importance in the diagnosis and pathogenesis of PCOS.
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Objective To investigate the expression of receptor for advanced glycation end products (RAGE) and soluble RAGE (sRAGE) in a rat model of dehydroepiandrosterone-induced polycystic ovary syndrome (PCOS) and to explore the role of sRAGE in ovarian function.Methods The PCOS rat model was established using dehydroepiandrosterone in combination with a high-fat diet.In animals with confirmed PCOS induction,serum concentrations of sRAGE and AMH were measured by competitive ELISA,and RAGE expression in ovarian tissues was detected by immunohistochemistry.Results RAGE expression was significantly higher in the PCOS group than in the control group (P ＜ 0.01).Serum concentrations of sRAGE and AMH were significantly lower and higher than those in the control group (P ＜ 0.05),respectively.Furthermore,sRAGE level was negatively correlated to that of AMH.Conclusion RAGE and sRAGE levels influence ovarian function and have substantial reference value in the diagnosis and pathogenesis of PCOS.
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Objective To observe the regulation of Fengxiaofang on the expression of gene Gob-5 in ovalbumin-challenged asthmatic mice. Methods The murine model of allergic asthma established by ovaibumin was used. The model mice were treated experimentally with Fengxiaofang and Dexamethasone as a positive control. The quantitive expression of gene Gob-5 in lung of mice was detected by real-time quantitative PCR. Results The expression of gene Gob-5 in lung of mice was down-regulated by Fengxiaofang. In the experiment, there were no significant differences between the one-fold or ten-fold dosage groups and the normal control group. Gene Gob-5 expression in five-fold dosage was significantly lower than that in the normal control group. Conclusion Fengxiaofang has the effect of lowering the expression of gene Gob-5 which maybe related to the potency on asthmatic disease.
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Objective To analyze the effect and mechanism of trichostatin A（TSA）on cell cycle in human ovarian cancer cells.Methods Human ovarian cancer cell line A2780 cells were cultured in RPMI 1640 supplement.Flow cytometry analysis and RT-PCR were used to examine the distribution of cell cycles and the level of p21WAF/CIPI mRNA.Results TSA induced increase of G2/M cells increased after the treatment of TSA for 36 hours（P 0.05）;the level of p21WAF/CIPI mRNA expression was upregulated after TSA treatment for 12 hours,the highest leve of its expression occurred at 24 hours,the expression level begun to decrease at 48 hours（P 0.05）.TSA simultaneously induced the decrease of S phase cells in a concentration-dependent manne（rP 0.05）.TSA upregulated the expression of p21WAF/CIPI mRNA in a concentration-dependent manner（P 0.05）.Conclusion TSA could block the G2/M phase and inhibits cell proliferation of A2780 cells through upregulating the expression of p21WAF/CIPI mRNA and the activate cyclin-dependent kinase.
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Objective To assess the effects of aspirin on the proliferation and apoptosis of human endometrial adenocarcinoma cells.Methods MTT assay was used to measure the effects of aspirin on the proliferation of Ishikawa cell.Flow cytometry(FCM) was employed to examine the distribution of cell cycles and the rates of apoptosis.Transmission electron microscopy was used to observe cell morphologic changes after aspirin administration.Results Aspirin inhibited the proliferation of cultured Ishikawa cells in a time-dependent and dose dependent manner(P<0.05).Aspirin increased the distribution of G,stage and the rates of cell apoptosis in a dose-dependent manner(P<0.05).Morphologic features of apoptosis cells,including cell shrinkage,nuclear condensation and apoptotic bodies could be found obviouslyunder the transmission electron microscopy.Conclusion Aspirin inhibited the proliferation and increased the apoptosis of human endometrial adenocarcinoma Ishikawa cells.
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Objective: To investigate the roles of Bcl-xl and Bcl-xs in the development and progression of endometrial carcinoma, and to explore their correlation.Methods: The expression of Bcl-xl and Bcl-xs in 32 cases of endometrial carci-noma, 12 cases of endometrial atypical hyperplasia, 6 cases of endometrial simple hyperplasia and 10 cases of normal en-dometrial tissues were examined by RT-PCR and Western-blot.Results: The expression of Bcl-xl mRNA and protein was significantly higher in endometrial cancer tissues than in normal endometrial tissues (P<0.05), and was statistically associat-ed with the pathological stage of endometrial carcinoma.(F=5.33, P=0.02).The expression of Bcl-xs mRNA and protein in atypical endometrial hyperplasia and endometrial carcinoma tissues were significantlly lower than that in normal endometri-al tissues (P<0.05), which was also associated with clinical stage and lymph node metastasis of endometrial carcinoma (P<0.05).The expression of Bcl-xl was negatively correlated with the expression of Bcl-xs in different endometrial tissues (r=-0.76).Conclusion: The abnormal expression of Bcl-xs and Bcl-xl was a factor for the pathogenesis and development of endometrial carcinoma.The negative correlation between Bcl-xl and Bcl-xs in different endometrial tissues as well as their relative expression ratio may have certain impact on the genesis of endornetrial cancer.
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Objective To find the changes and effects of nitric oxide synthase activity in atherosclerotic rabbit models. Methods Thirty rabbits were divided into 3 groups at random: control group, atherogenic diet group, balloon-injury+ atherogenic diet group. Three months later underwent pharmacological triggering with Chinese Russell’s viper venom and histamine to make the plaque rupture. Lipid concentrations and the levels of nitric oxide (NO), NOS and hydroxy radical (OH) in the serum were obtained at different period, and pathologic changes were observed. Results In atherogenic diet group and balloon-injury+ atherogenic diet group, serum lipid concentrations markedly increased, but the levels of NO did not show any difference. The activity of NOS and OH levels were obviously increased after the atherogenic diet. But it reduced after pharmacological triggering and plaque rupture. Conclusion In early period of atherosclerosis, NOS activity up regulated and endothelium functions were compensated. But, When the atherosclerotic plaques were unstable, the activity of NOS descended obviously and endothelium function goes into incompensation.