ABSTRACT
Henoch-Sch?nlein purpura(HSP)is an IgA-associated leukocytoclastic small-vessel vasculitis, commonly occurred in children.Recent studies have shown that its pathogenesis is closely related to the disturbance of humoral immune response mediated by B cells.CD4 + T cells have the function of help B cells.Each subgroup of CD4 + T cells has unique functions of promoting or regulating immune inflammatory response.There are abnormal changes in the proportion of CD4 + T cells subsets in HSP patients, including the imbalance of Th1/Th2, increasing numbers of Th17 and Tfh cells, together with decreasing Treg cell numbers.The changes are closely related to the occurrence, development and the clinical phenotype of HSP.It is suggested that the detection of CD4 + T cells plays critical roles in guiding the evaluation of HSP, and is expected to provide a new idea for the clinical diagnosis and individualized treatment of HSP.
ABSTRACT
<p><b>OBJECTIVE</b>To study the change of endogenous metabolites of SD rats administrated of aqueous extract of Evodiae rutaecarpa.</p><p><b>METHOD</b>Six SD rats had been successively administrated aqueous extract of E. rutaecarpa (0.3857 g x kg(-1)) for 33 days. An agilent 1200 6410 triplequadrupole mass spectrometer was used for the analysis of endogenous metabolites in rat urine samples. These data was analyzed by the principal component analysis (PCA) and PLS-DA using the SIMCA-P 10.0 software.</p><p><b>RESULT</b>The significant difference in metabolic profiles between the control group and the dosed group was well observed by PCA of the MS data.</p><p><b>CONCLUSION</b>The E. rulaecarpa has changed the endogenous metabolites of SD rats. This work can provide the base for the further research on the interpretation of drug property of E. rulaecarpa.</p>
Subject(s)
Animals , Female , Male , Rats , Evodia , Chemistry , Gene Expression , Metabolomics , Methods , Plant Extracts , Pharmacology , Principal Component Analysis , Rats, Sprague-DawleyABSTRACT
Objective To construct 2 recombinant plasmids carrying cassette chromosome recombinase C(ccrC)and ccrAB respectively and introduce the plasmids into methicillin-resistant Staphlococcus aureus(MRSA)strain 81/0432,and to observe the precise excision of Staphylococcal cassette chromosome mec(SCCmec)island from bacterial chromosome.Methods ccrC and ccrAB genes were amplified with chromosomal DNAs isolated from MRSA strains 81/0342 and N31S as PCR templates.We constructed recombinant plasmids pSR5C and pSR2AB by cloning ccrC and ccrAB genes into temperature-sensitive plasmid pYI3,after introducing them into MRSA strains 81/0432 and N315 by electroporation.PCR was performed to identify SCCmec excision from the bacterial chromosome.The transformants were serial passaged for 10 days,and then the drug resistance of these rransformants was detected by replica experiment.Results The fragment length of ccrC gene was 1.9 kb,smaller than the fragment length of ccrAB from N315.The recombinant plasmids of pSR5C and pSR2AB were successfully constructed.After these 2 recombinant plasmids were introduced into MRSA strain 81/0342,type-V SCCmec island was excised from the chromosome and formed a closed circular structure in the bacteria.However,type-Ⅱ SCCmec island could be excised only in N31S strain after the expression of pSR2AB.Replica experiment verified that transformed strains of 0342(pSR2AB),0342(pSR5C),and N315 (pSR2AB)were mostly susceptible to ceftizoxim or tobramycin after the excision of SCCmec island.Conclusion cciC could serve as a recombinase as ccrAB,which could mediate precise excision of SCCmec island from the chromosome of type-V MRSA strain.This study shows that type-V SCCmec island is widely disseminated between Staphylococcus aweus strains in community setting.
ABSTRACT
<p><b>OBJECTIVE</b>HPLC-MS/MS-based metabonomics method was used to find the possible biomarker of Rhizoma Coptidis in rat urine.</p><p><b>METHOD</b>Sprague-Dawley rats were successively administrated 7 g x kg(-1) aqueous extract of Rhizoma Coptidis for 30 days, urine were collected by metabolism cages and detected by using the HPLC-MS-MS. All dates were analyzed by the principal component analysis (PCA) through using the SIMCA-P 10.0 software.</p><p><b>RESULT</b>The PCA demonstrated that the metabolome between treated group and control group had difference in rat urine sample after of 22 days administrated, for treated group 169 kinds of biomarkers were found including oxalacetic acid, malic acid, 2-ketoglutaric acid, NE, arachidonic acid, 5-HIAA and other compounds, the result was consistent with pharmacological effects of R. coptidis, such as antiinflammatory, inhibiting biosynthesis of CA biosynthesis, anticentral nerve and energy metabolism inhibition.</p><p><b>CONCLUSION</b>Metabonomics may be available in pharmacological action evaluation of drugs.</p>