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Ninety eight patients with chronic hepatitis B (CHB) admitted to Department of Infectious Diseases, Yongkang First People′s Hospital from January 2017 to June 2018 were randomly divided into two groups. On the basis of entecavir+dicyclool treatment, patients in control group (48 cases) received health education and weekly telephone follow-up from the nurses, while patients in intervention group (50 cases) received health guidance from doctors and psychological counseling and communication from the nurses. All patients were followed up for 8 weeks. The illness perception, negative emotions, quality of life, and medication compliance were evaluated with the Revised Illness Perception Questionnaire (IPQ-R), Self-rating Anxiety Scale (SAS), Self-rating Depression Scale (SDS), SF-36 and Morisky Medication Adherence Scale (MMAS-8) in two groups, respectively. The results showed that, after 8 weeks, the disease identity, serious consequences, treatment control and disease correlation; SAS and SDS scores; physiological function, emotional function, energy and social function were significantly improved in control group( t=2.30, 3.45, 2.35, 2.23, 5.04, 6.03, 2.03, 2.79, 2.02, 2.34, all P<0.05). All dimension of illness perception; SAS and SDS scores; physiological function, physiological function, emotional function, energy, mental health, social function and general health were also improved significantly in intervention group ( t=4.06, 5.44, 6.91, 8.53, 5.65, 8.77, 7.25, 6.06; 12.71, 11.91, 2.27, 2.60, 4.48, 2.82, 3.65, 3.461, 2.82,all P<0.05). And the personal control, disease correlation and emotional expression; SAS and SDS scores; emotional function and mental health in intervention group were better than those in control group ( t=1.49, 2.74, 2.60, 5.02, 5.18, 5.56, 13.72, all P<0.05). In addition, the medication compliance of patients in both groups was significant improved ( Z=5.56, 13.72, all P<0.01), and the improvement was more marked in the intervention group ( Z=3.22, P<0.01). The study indicates that the comprehensive psychotherapy can more significantly improve the degree of illness perception, negative emotions, quality of life and medication compliance in patients with chronic hepatitis B.
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Background and Objectives@#Bone marrow mesenchymal stem cells (BMSCs) is an ideal source of stem cells in the treatment of intrauterine adhesion. Exosomes are a type of membrane vesicle and the diameter is 30∼100 nm. Exosomes can take their contents into the target cells, releasing and exerting their functions. In this study, we intend to study the role of human BMSC-derived exosomes (BMSC-Exo) in promoting endometrial damage repair in the treatment of IUA. @*Methods@#We used the magnetic bead affinity method to extract BMSC-Exo and analyzed its biological character. Then we co-cultured the BMSCs-Exo with endometrial cells to detect its effect. We injected BMSCs-Exo into the IUA mouse model. We over-expressed miR-29a in BMSCs-Exo by transient transfection, then used RT-PCR to analyze the expression of the related genes. @*Results@#BMSCs-Exo expressed exosome-specific proteins CD9, CD63, and CD81. BMSCs-Exo could bring the contents into the target cells. BMSCs-Exo can promote endometrial repair in vitro or in vivo. BMSCs-Exo overexpressing miR-29a can reduce αSMA, Collagen I, SMAD2, and SMAD3. @*Conclusions@#In this study, we successfully isolated BMSCs-Exo and proved its character and biological activity. BMSCs-Exo can promote cell proliferation and cell migration in vitro and can repair damaged endometrium in the IUA model. The presence of miR-29a in BMSCs-Exo may be an important factor in its resistance to fibrosis during endometrial repair of IUA. This study provides new ideas for the treatment of patients with IUA and has important clinical research significance.
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Objective To analyze the different clinical effects of colpohysterectomy combined with anterior and posterior wall colporrhaphy with or without biological mesh for uterine prolapse.Methods From January 2010 to May 2015, colpohysterectomy combined with anterior and posterior wall colporrhaphy was performed in 106 cases of uterine prolapse, including 75 cases receiving biological mesh repair (experimental group) and 31 cases of non-biological mesh (control group).Clinical and surgical information of all patients was collected, and satistial analysis was done between the two groups.Results All the operations were accomplished successfully.There were no statistical differences in bleeding amount during operation [(34.5±7.3) ml vs.(32.1±4.9) ml, t=1.973, P=0.051], time to first flatus [(29.2±4.8) d vs.(30.2±5.3) d, t=-0.907, P=0.366], postoperative hospital stay [(6.1±1.5) d vs.(6.0±1.4) d, t=0.328, P=0.744], as well as the operative time [(91.4±3.4) min vs.(95.1±10.2) min, t=-1.970, P=0.051].At one year after surgery, in the experimental group there were 74 patients cured, 1 patient improved, and no invalid or recurrence case, while in the control group there were 27 patients cured, 1 patient improved, 3 cases of recurrence and no invalid case.The treatment effect of the experimental group was significant better than that of the control group (Z=-2.571, P=0.010).Conclusions Colpohysterectomy with anterior and posterior wall colporrhaphy with biological mesh can be selected for uterine prolapse patients without contraindications.The results of surgery are better with biological mesh, with low recurrence rate.
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Objective To evaluate the effects of electric coagulation or suture hemostasis on ovarian reservation function in laparoscopic ovarian cyst removal for ovarian endometriomas. Methods A total of 118 patients receiving laparoscopic ovarian cystectomy in our hospital from January 2011 to June 2015 were enrolled in this study.According to the habit of different operators,the patient were devided into either electric coagulation group(n =53)or suture group(n =65).The follicle-stimulating hormone (FSH),luteinizing hormone(LH),estradiol(E2)and antral follicle count(AFC)of the two groups were observed and compared before surgery and one and six months after surgery. Results There were no differences in the levels of the FSH,LH,E2and AFC between the two groups before the surgery(P>0.05).The level of FSH was increased and those of E 2and AFC were declined in both groups at 1 month after the operation compared with those before operation(P <0.05).Moreover, there were more significant differences in FSH,E2,and AFC in the electric coagulation group(P <0.05).There was no significant difference in LH(P>0.05).The levels of FSH and E 2recovered in both groups at 6 months after operation.There was no difference in FSH and E 2 compared with the level before operation in the suture group(P>0.05), however, there was significant difference in the electric coagulation group(P<0.05). Conclusions The laparoscopic ovarian cystectomy for ovarian endometriomas leads to decreased ovarian reserve.As compared to bipolar coagulation, suture hemostasis can effectively reduce the damage of ovarian function, which should be applied as possible as we can.
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Objective To evaluate the effects of electric coagulation or suture hemostasis on ovarian reservation function in laparoscopic ovarian cyst removal for ovarian endometriomas. Methods A total of 118 patients receiving laparoscopic ovarian cystectomy in our hospital from January 2011 to June 2015 were enrolled in this study.According to the habit of different operators,the patient were devided into either electric coagulation group(n =53)or suture group(n =65).The follicle-stimulating hormone (FSH),luteinizing hormone(LH),estradiol(E2)and antral follicle count(AFC)of the two groups were observed and compared before surgery and one and six months after surgery. Results There were no differences in the levels of the FSH,LH,E2and AFC between the two groups before the surgery(P>0.05).The level of FSH was increased and those of E 2and AFC were declined in both groups at 1 month after the operation compared with those before operation(P <0.05).Moreover, there were more significant differences in FSH,E2,and AFC in the electric coagulation group(P <0.05).There was no significant difference in LH(P>0.05).The levels of FSH and E 2recovered in both groups at 6 months after operation.There was no difference in FSH and E 2 compared with the level before operation in the suture group(P>0.05), however, there was significant difference in the electric coagulation group(P<0.05). Conclusions The laparoscopic ovarian cystectomy for ovarian endometriomas leads to decreased ovarian reserve.As compared to bipolar coagulation, suture hemostasis can effectively reduce the damage of ovarian function, which should be applied as possible as we can.
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Objective To evaluate the clinical efficacy of laparoscopic surgery combined with GnRH agonist in the treatment of endometriosis. Methods From January 2008 to January 2012, 205 patients with endometriosis underwent conservative laparoscopy surgery in our hospital .According to whether the patients accepted the therapy of GnRH-a and the time of the treatment after surgery, patients were divided into three groups:group A was treated with conservative laparoscopic surgical (73 cases), group B was given GnRH-a for 3 months after surgery (68 cases), and group C received GnRH-a for 6 months after surgery (64 cases).These patients were followed up for 24 months after surgery .The recurrence rate and pregnancy rate were compared among the three groups . Results The postoperative recurrence rates within one year were 4.4%(3/68) in the group B and 3.1% (2/64) in the group C, which were all lower than that of the group A (13.7%, 10/73).There was no difference between the group A and B (χ2 =3.628, P=0.057), while the rate of group C was significantly lower than that of the group A (χ2 =4.771, P=0.029).The cumulative recurrence rates in the group B and C were respectively 13.2%(9/68) and 10.9%(7/64), which were significantly lower than that of the group A [27.4%(20/73); χ2 =4.322, P=0.038; χ2 =5.839, P=0.016].There were no differences about the natural pregnancy rate within 2 years postoperatively among the three groups (χ2 =0.812, P=0.666), but for those who underwent IVF-ET after laparoscopy, the pregnancy rates in group B and C were respectively 78.9% (15/19) and 80.0% (16/20), which were significantly higher than that of the group A [47.6% (10/21); χ2 =4.177, P=0.041; χ2 =4.630, P=0.031]. Conclusions Laparoscopic surgery combined with GnRH-a is highly effective in the treatment of endometriosis , which can effectively reduce the recurrence rate .Application of GnRH-a for 3-6 months after laparoscopic surgery can improve the pregnancy rate in those who accept the treatment of IVF-ET.
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Objective To investigate two different hysterectomy ( total abdominal hysterectomy and total laparoscopic hysterectomy ) on short-term efficacy and influence on sexual functions . Methods The retrospective records of 100 cases of total laparoscopic hysterectomy ( TLH) and 100 cases of total abdominal hysterectomy ( TAH) from January 2009 to December 2012 were reviewed.The operation time, intraoperative blood loss, postoperative hospital stay, and sexual satisfaction at 12 months postoperatively were compared between the two groups . Results The operation time was longer in the TLH Group than that in the TAH Group [(128 ±11) min vs.(87 ±33) min, t=-11.787, P=0.000].The intraoperative blood loss was less in the TLH Group than that in the TAH Group [(108 ±37) ml vs.(155 ±28) ml, t=-10.129, P=0.000].The hospital stay was shorter in the TLH Group than that in the TAH Group [(5.5 ±1.9) d vs.(8.2 ±1.6) d, t =-10.870, P =0.000].There were no significant differences between the two groups in sexual frequency (Z=-1.300, P=0.193), libido (Z=-0.564, P=0.573), achievement of orgasm (Z =-1.591, P=0.112), sexual intercourse disorder (Z =-0.478, P =0.633), and the overall satisfaction (Z=-0.083, P=0.934).Extent of dyspareunia was worse in the TLH Group than in the TAH Group (Z=-3.752, P=0.000). Conclusions TLH has less blood loss and shorter hospitalization time than TAH .Hysterectomy has a certain influence on sex functions .Differences in the sexual satisfaction are not statistically significant between the two procedures .
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Objective-To-study-the-clinical-characteristics-of-laparoscopic-oviduct-anastomosis-for-the-treatment-of-fallopian-tube-sterilization-or-tubal-infertility-.-Methods-A-total-of-48-cases-of-fallopian-tube-sterilization-or-tubal-infertility-patients-underwent-oviduct-anastomosis-between-January-2006-and-August-2013-in-our-hospital-.The-patients-were-divided-equally-into-laparoscopic-surgery-group-(laparoscopic-group,-24-cases)-or-open-surgery-group-(open-group,-24-cases)-in-chronological-order.The-ratio-of-infertility-patient-was-equal-in-both-laparoscopic-surgery-group-and-open-surgery-group-.Repatency-rate-and-pregnancy-rate-of-different-surgical-procedures-were-analyzed-,-respectively-.-Results-All-the-operations-were-successfully-completed-.Bilateral-tubal-repatency-rate-was-100%in-all-the-patients-during-the-operation-.The-pregnancy-rate-of-laparoscopic-surgery-group-(87.5%,21/24)-was-higher-than-that-in-the-open-surgery-group-(62.5%,15/24)-within-24-months-after-surgery,-with-significant-difference-(χ2-=4.000,-P=0.046).-Conclusions-The-success-rate-of-laparoscopic-oviduct-anastomosis-is-comparable-to-open-surgery-.However-,-the-pregnancy-rate-after-laparoscopic-oviduct-anastomosis-is-higher-than-open-surgery-.Laparoscopic-oviduct-anastomosis-has-a-broad-prospect-of-application-.
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This paper is focused on head modeling using the sophisticated realistic finite element method (FEM) with five-layer tissues based on segmented data. First, the location of every pixel of the FEM head model was fixed on according to the registration method based on the magnetic resonance imaging (MRI) and computer tomography (CT) images. Then the location information was rebuilt with the hexahedron pattern in the ANSYS FEM software. Finally, the forward problem numerical computation was performed on this FEM head model. The simulation results verified the rationality and reliability of the model applied on the electroencephalograph (EEG)/magnetoencephalograph (MEG) study. The hexahedron meshing realistic head model combined with the MRI scanner information has potential in the future research of EEG/MEG.
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Humans , Brain , Diagnostic Imaging , Physiology , Electroencephalography , Methods , Finite Element Analysis , Imaging, Three-Dimensional , Methods , Magnetic Resonance Imaging , Methods , Models, Biological , Skull , Diagnostic Imaging , Physiology , Tomography, X-Ray Computed , MethodsABSTRACT
Objectives This paper is focused on the investigation of effects of inhomogeneous head tissues and anisotropic skull as well as white matter conductivity distribution on magnetic and electric field distribution of electric impedance tomography (EIT) and magnetic resonance electrical impedance tomography (MREIT) based on the five-layer FEM head modeling.Methods Four different characteristics head models were set:homogeneous distribution,inhomogeneous distribution,anisotropic skull and white matter distribution.Electric and magnetic field distribution were obtained by the performance of numeric solving of the forward problem,and the effects of inhomogeneous conductivity and anisotropic conductivity on the distribution were studied by applying the quantitative statistical analysis.Results The present results showed that inhomogeneous and anisotropic characteristics can significantly alter the electric field and current density distributions then the magnetic field distribution induced in the brain.Conclusion The conclusion provides theoretical basis for the application of MREIT in the reconstruction of inhomogeneous and anisotropic conductivity of head tissues.
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The study intended to evaluate the efficacy and safety of patient-controlled intravenous analgesia with remifentanil for labor. Three hundred and two parturients at 37 -42 week of gestation received remifentanil (0. 1μg · kg-1 ·min-1 ) as patient-controlled intravenous analgesia for labor (analgesia group) and their clinical results were compared with those of 221 parturients in labor without intravenous analgesia (control group). Maternal monitoring, including pain scores(VAS), delivery mode, side effects, laboring time was performed for both groups. There was a significant decrease (P ＜0. 01) of pain scores in analgesia group compared with those in control group. Parturients in analgesia group experienced a significantly shorter first stage, than those in the control group ( P ＜ 0. 01). There were no differences between two groups in the rate of cesarean section and instrumental delivery. And there were no significant differences for newborns with Apgar scores less than 7 at the point of one minute. Patient-controlled intravenous analgesia with remifentanil for labor is an effective and safe procedure, which can significantly relieve pain, shorten first stage of labor and has no effect on the delivery mode and Apgar scores of neonates.
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Objective To investigate the relationship between the maternal nutritional stetus and the occurrence of intrauterine growth retardation (IUGR) with neonatal visual development disorder Methods Sixty pregnant women closed to expected date of confinement were randomly divided into 2 groups: IUGR and normal control group, each were with 30 cases Eight kinds of nutritional foods taken by the pregnant women were investigated and scored The vitamin A concentrations of maternal and umbilical blood were measured with fluorometer method The concentrations of iron, copper and zinc in maternal and umbilical blood were detected by spectrophotometer The results of examination with Flash visual evoked potentials (F VEP) were recorded by an experienced technician in neonates at 3～7 days after birth and 10 months after follow up Results Compared with the normal control, the IUGR group had taken less eggs, animal liver and fat pork during pregnancy ( P