A study of the practice of "three early education" combined with medical ethics literacy cultivation to improve medical students' post competence / 中华医学教育探索杂志

Clinical post competence and medical ethics literacy are the key qualities that applied medical talents should have. The synergy of medical education is an effective way to deepen the reform of competence-oriented medical education. Under the background of synergy of medical education and considering the orientation of training applied medical talents in our college, we explore a progressive path of "three early education", which means early exposure to specialties, early exposure to practice, and early exposure to clinical practice. Meanwhile, we strengthen the cultivation of medical students' medical ethics literacy throughout the whole process of "three early education", laying a solid foundation for medical students before they enter the stage of clinical practice training. Practice shows that the progressive "three early education" mode and the cultivation of medical ethics literacy throughout the whole process can effectively improve medical students' clinical post competence.

Anatomy study and clinical application of minimally invasive anterior lumbar retroperitoneal approach / 中华骨科杂志

Objective:To explore the ideal method of minimally invasive anterior lumbar extraperitoneal approach.Methods:Twenty-one adult embalmed cadavers underwent longitudinal incision near the left rectus abdominis, the extraperitoneal space and peritoneal characteristics were observed; the L 2-S 1 disc was exposed through extraperitoneal approach, and the relationship between the anterior large vessels and the disc was observed. One hundred adult abdominal CT were collected to measure the distance between the extraperitoneal fat of anterior abdominal wall and the rectus abdominis and the anterior midline at L 2-S 1 segment. One hundred and fifty adult lumbar MRI were collected to measure the distance between the anterior great vessels and the anterior midline of the intervertebral disc. Fifty-six cases of lumbar fusion were performed by minimally invasive anterior lumbar extraperitoneal approach, including 25 males and 31 females, aged 29-71 years. L 2-L 4 in 8 cases was performed by left rectus abdominis oblique incision, and L 4-S 1 in 48 cases was performed by median left transverse incision, with a length of about 8 cm, the complications related to the surgical approach were evaluated. Results:L 2-L 4 was proximal to the arcuate line, the posterior sheath of rectus abdominis adhered to the peritoneum, which was easy to rupture when separated; the peritoneum gradually thickened from the outer edge of the sheath of rectus abdominis and extraperitoneal fat appears. L 4-S 1 could be exposed distal to the arcuate line, the posterior side of rectus abdominis was extraperitoneal fat, the extension of arcuate line to the lateral abdominal wall would be slightly separated proximally, and there were multiple iliopsoas veins in the medial side of psoas major muscle. L 5S 1 was between the right common iliac artery and the left common iliac vein far, the median sacral vessel was small or absent, and the sympathetic nerve was to the left. Extraperitoneal fat appeared 36.2±9.9 mm, 35.2±11.6 mm and 27.6±11.2 mm away from the outer edge of rectus abdominis at L 2, 3, L 3, 4 and L 4, 5 segments respectively, and covered the posterior side of rectus abdominis and reached the midline at L 5S 1 segment. The left edge of abdominal aorta was 14.9±5.1 mm, 13.9±4.6 mm and 19.7±5.9 mm away from the midline at L 2, 3, L 3, 4 and L 4, 5 level respectively; the inferior vena cava was located on the right side of the midline at L 2, 3 and L 3, 4 level, crossed the midline 4.6±8.7 mm at L 4, 5 level. At L 5S 1 level, the left common iliac vein and the right common iliac artery were 14.6±6.8 mm and 17.6±5.3 mm away from the midline respectively. Seventy-six patients were successfully and fully exposed by small incision through extraperitoneal approach. 1 case of L 4, 5 had iliac lumbar vein tear and hemostasis with bipolar electrocoagulation. The operation time was 70-120 min, with an average of 90 min; Intraoperative bleeding was 15-70 ml, with an average of 30 ml. No severe complication such as nerve and great vessel injury occurred. Conclusion:Minimally invasive lumbar anterior retroperitoneal approach has small trauma and sufficient exposure with good feasibility. L 2-L 4 can be exposed with supine position and oblique incision next to the left rectus abdominis muscle, and L 4~S 1 with French position and median left transverse incision.

Research progress in molecular pathology of salivary gland carcinoma and clinical application / 国际生物医学工程杂志

Salivary gland cancer includes a group of heterogeneous malignant tumors. The latest WHO classification of head and neck tumors divides salivary gland cancer into 22 histopathological subtypes. The most common clinical subtypes include mucoepidermoid carcinoma, adenoid cystic carcinoma, salivary duct carcinoma, acinar cell carcinoma and secretory carcinoma, etc. The histomorphology of the subtypes of salivary gland cancer overlaps, making diagnosis and differential diagnosis difficult. The main treatment for salivary gland cancer is tumor resection and postoperative radiotherapy as appropriate. Even in cases of local progression, recurrence and metastasis, the treatment options available are limited, mainly palliative treatment. Accurate determination of tumor receptor expression, genome and pathway changes is the key to changing the prognosis of patients with local progression or distant metastasis. In this paper, the known genetic mutations, amplifications and protein expression profiles of common salivary gland cancer histological subtypes was summarized, and the significance of the above-mentioned molecular genetic changes for disease diagnosis, future research and personalized targeted therapy was analyzed.

Analysis on the survey results of iodine content in drinking water in Fuzhou, 2017 / 中华地方病学杂志

Objective:To understand the level and distribution of iodine contents in drinking water in Fuzhou City so as to provide evidence for prevention of iodine deficiency disorders and health intervention.Methods:In 2017, a township-level water iodine survey was carried out in 12 counties (cities, districts) under the jurisdiction of Fuzhou City, with a township as a unit, and carry out administrative village-level water iodine survey in township with the median water iodine ≥ 10 μg/L. The water samples of residents' drinking water were collected according to different water supply methods (centralized water supply, mixed water supply and decentralized water supply), and the water iodine content was determined. The water iodine content less than 10 μg/L means iodine deficiency in the external environment. The iodine contents in drinking water of residents in different areas, different water supply methods and different water source types were compared and analyzed.Results:A total of 172 townships in 12 counties (cities, districts) were investigated for water iodine, and 1 630 water samples were collected and tested, the median water iodine was 2.8 μg/L and the range was 0.0 - 130.3 μg/L. At the township-level, the median range of water iodine was 0.4 - 11.2 μg/L. A total of 75 administrative villages were investigated in 4 townships with a median water iodine ≥ 10 μg/L, the median water iodine range was 0.5 - 19.8 μg/L. At the counties (cities, districts) level, the medians of water iodine were all less than 10 μg/L. Among them, the medians of water iodine in inland areas and coastal areas were 2.0 and 4.0 μg/L, respectively, and the difference between different areas was statistically significant ( Z = - 15.70, P <0.01). The medians of water iodine for centralized water supply, mixed water supply and decentralized water supply were 4.1, 2.7, and 3.4 μg/L, respectively, and the difference between different water supply methods was statistically significant ( H = 19.38, P < 0.01). The medians of water iodine in surface water and well water were 2.4 and 3.1 μg/L, respectively, the difference between different water source types was statistically significant ( Z = - 6.23, P<0.01). Conclusions:Iodine deficiency of external environment is common in Fuzhou City, and there is no distribution of high iodine in water source. It is necessary to strengthen the monitoring of iodine deficiency disorders, establish a long-term working mechanism suitable for the level of economic and social development, and consolidate the achievements of prevention and control.

Clinical curative effect research status and analysis of acupuncture treatment for primary insomnia / 中国针灸

<p><b>OBJECTIVE</b>To grasp the clinical efficacy of acupuncture in the treatment of primary insomnia (PI), and analyze its future research directions.</p><p><b>METHODS</b>A total of 41 prospective randomized controlled trials (RCTs) were conducted in which the clinical efficacy of acupuncture for treatment of PI was compared with sedative and hypnotic drugs in recent six years by searching databases of CNKI, database, PubMed, and BioMed Central (BMC), the aspects of diagnostic criteria, efficacy standards, observation time, control drugs, characteristics of acupoint selection and regularity were used to review and analyse.</p><p><b>RESULTS</b>Acupuncture treatment for PI was mainly based on acupoints in the head, combined with selecting the points according to the different syndrome, showing short-term efficacy and safety advantages, but it was not well-established in many aspects such as diagnostic criteria, efficacy evaluation, observation time, and control drugs.</p><p><b>CONCLUSION</b>Current evidence shows that acupuncture treatment is effective, but it is necessary to add more stringent RCTs, and introduce objective monitoring indicators to strengthen the evidence and enhance the overall level of research.</p>

The Changes of Neurocognitive Function in Early Stage in Patients with First-Episode Schizophrenia / 现代生物医学进展

Objective:To evaluate the changes of neurocognitive function in early stage in patients with first-episode schizophrenia.Methods:In this study,73 cases of patients with first-episode schizophrenia (research group) and 75 cases of health person (control group) were selected from January 2015 to January 2016 in our hospital.The neurocognitive function was evaluated by neuro-psychological testing tool and the data between two group were compared.Results:Scores of delayed recall,total recall,3 trial,2 trial and 1 trial of research group were lower than those of control group in BVMT-R test,and the difference was statistically significant (P＜0.05).In HVLT-R test,the scores of total delay,3 trial and 2 trial of research group were significantly lower than those of control group (P＜0.05).The consuming time of dominant hands and subdominant hands in pegboard tasks were significantly higher in research group than in control group (P＜0.05).Conpletion time of connection test A,color connection 1 and 2 in connection test of research group were significantly higher than those of control group (P＜0.05).Attempt number and correct number in research group in PASAT test were significantly lower than in control group (P＜0.05).Number of search errors in research group was higher than in control group,while number of search correct,search total score and digital sign score were significantly lower than in control group (P＜0.05).Total number of words,color and color / word count in research group were lower than in control group in Stroop color word test,and the difference was statistically significant (P＜0.05).WMS-Ⅲ test results between two group had no significant difference (P＞0.05).Conclusion:The neurocognitive function in early stage in patients with first-episode schizophrenia has been extensively damaged.

Study on Extraction and Inclusion Processes of Mixed Volatile Oils from Forsythiae Fructus, Saposhnikoviae Radix and Magnoliae Flos / 中国中医药信息杂志

Objective To optimize the extraction process of mixed volatile oil from Forsythiae Fructus, Saposhnikoviae Radix and Magnoliae Flos and inclusion process of β-cyclodextrin (β-CD). Methods With yield ratio of volatile oil as evaluation index, single factor experiments were used to study the extraction process of volatile oil;saturated aqueous solution was used, with inclusion rate of volatile oil as index, and orthogonal design was adopted to examine effects of charge ratio of volatile oil and β-CD, inclusion temperature and inclusion time on the inclusion process; X-ray scattering technique, infrared spectroscopy and scanning electron microscopy were used to characterize the inclusion compound. Results The optimum extraction process of volatile oil was soaking fine powder extracted 5 hours with 10 folds the amount of water. The optimum conditions of inclusion process were as follows:mixed ratio of volatile oil (mL) and β-CD (g) was 1:10; inclusion temperature was 50 ℃; the inclusion time was 2 h. X-ray scattering technique, infrared spectroscopy and scanning electron microscopy proved the inclusion compound had been formed. Conclusion Optimum extraction and inclusion processes are stable and feasible, and can provide research foundation for further research and development of preparation.

Status and influencing factors of professional self-concept of nurses / 中国实用护理杂志

Objective To investigate the status of professional self-concept of nurses and its influencing factors of Clinical registered nursing major in Guangdong. In order to provide the basic theory of improving the level of professional self-concept of nurses. Methods A total of 280 nurses were investigated from different levels and deployment of hospitals by convenient sampling method and with Nursing Professional Self-concept Scale (PSCNI).With variance analysis, two-sample t test and multiple stepwise regression analysis to analyze the influence of professional self-concept of nurses by demographic questions. Results The score of professional self-concept of nurses was (85.79±9.23) points, the project average score was (2.86 ± 0.31) points which were positive. Dimension score of flexibility was the highest which was (3.23±0.44) points. Dimension score of communication skills was the lowest which was (2.62± 0.38)points. Age、years of work, marital status, job title, whether hold executive duties、the choice of career, job satisfaction (t/F=1.29, 1.53, -3.13, 1.88, 3.68, 3.59, 1.68, P<0.01)all had a significant impact on professional self-concept of nurses. And age, the choice of career and job satisfaction (t=4.385,-2.889,-2.268, P=0.000, 0.004, 0.024)were three main related factors of the score of professional self-concept. Conclusions The professional self-concept of nurses was positive, but it is also unbalanced. Nursing managers should be concentrate on increasing the level of professional self-concept of young and involuntary nurses, and also according to the characteristics of hospitals and departments to improve the job satisfaction of nurses to achieve the purpose of improving the professional self-concept of nurses themselves.

The analysis of clinical application on children′s respiratory tract infection pathogens IgM antibody nine-combined detection in Pu′er / 国际检验医学杂志

Objective To analyze the results of children′s respiratory tract infection pathogens IgM antibody nine‐combined de‐tection in Pu′er and grasp the epidemiological characteristics of respiratory tract infection of local children for providing reference for clinical diagnosis and treatment .Methods 1 327 cases of children′s blood sample with respiratory tract infection were randomly sampled from in Pu′er People′s Hospital in 2014 .All the samples were tested with nine respiratory tract infection pathogens IgM antibody detection reagent kit(indirect immunofluorescence) ,and the test results were adopted for statistical analysis .Results There were 446 cases positive for IgM antibody in 1 327 cases of respiratory tract infection children ,the total positive rate was 33 .6% .The Positive rate of mycoplasma pneumonia(MP) was the highest ,for 23 .6% .The second was influenza B virus(INFB) , for 18 .5% .The eosinophilic lung legionella type 1(LP) ,adenovirus(ADV) ,respiratory syncytial virus(RSV) ,influenza A(INFA) and parainfluenza virus(HPIVs) were all much lower :respectively for 0 .4% ,1 .1% ,1 .0% ,1 .1% and 1 .0% .It was showed that no positive results were found in Q fever rickettsia(CB) and chlamydia pneumonia(CP) .There was significant difference of the positive rate among 9 kinds of pathogens(P<0 .05) .There was significant difference of the monthly positive rate among the main six patho‐gens(P<0 .05) .There was significant difference of the positive rate in the different age groups(P<0 .05) .Conclusion The respir‐atory tract infection pathogens of children in Pu′er are mainly mycoplasma pneumoniae and influenza B virus .The epidemiological distribution of the pathogens is related to the age and season ,the clinician should reasonably diagnose and treat according to the test results for children .

Epidemiological investigation on TORCH infection of children in Pu′er / 国际检验医学杂志

Objective To make an epidemiological investigation on TORCH infection in children in Pu′er city of Yunnan prov‐ince ,in order to provide references for the prevention and treatment of TORCH infection in children .Methods A total of 1 194 children treated at this hospital from January to December 2014 were selected and divided into five age groups .Serum TORCH spe‐cific IgM and IgG antibodies were detected by using enzyme‐linked immunosorbent assay(ELISA) and the positive rate of TORCH specific antibody and TORCH infection rate were comparatively analysed .Results The positive rate of TORCH specific IgM anti‐body was significantly lower than that of IgG .The range of positive rate of IgM was 0 .00% -3 .10% ,and that of IgG was 10 .13% -82 .24% .The total infection rate of TORCH was 96 .98% .There were no significant differences in the infection rate of single item of TORCH or the total TORCH infection rate among the five different age groups(P>0 .05) .The distribution of infec‐tion rate of single item of TORCH among the five age groups was statistically significantly different(P0 .05) .Conclusion Children with TORCH infection in this area mainly are infected by CMV ,followed with RV and HSV infections ,while TOX infection is uncommon .There are no obvious differences in different seasons and children in different age groups .

Analysis of the application effect with energy spectrum CT multi -parameter quantitative values in pulmonary qccupying lesions / 中国基层医药

Objective To evaluate the application value with Energy Spectrum CT multi -parameter quanti-tative value in differentiating pulmonary occupying lesions (>0.5cm in diameter).Methods Those were retrospec-tively analyzed on 58 cases in pulmonary occupying lesions performed spectrum dual -phase enhanced CT scan and confirmed by pathology,including malignant group 48 cases;10 cases of benign group.Iodine content(IC)was meas-ured in arterial phase(AP)and venous phase (VP)in iodine based on the image and the level of the aorta,and nor-malized iodine concentrations were calculated in the two phases lesions compared with aorta,and the difference between the two normalized iodine concentrations ICD (ICD =NICvp -NICap).To measure the CT value of lesions on 50keV and 100keV energy image,and calculate the energy attenuation curve slope,namely |Hu (100 kev -50kev)/Hu50 |.The differences of NIC,curve slope,and ICD were compared between benign and malignant pulmonary occupying lesions using independent sample t test method.Results In two phase scan,NIC,curve slope of the venous phase and ICD in malignant group were significantly higher than the benign group,(NIC,curve slope of the venous phase and ICD of the malignant group:NICap:0.180 ±0.051,NICvp:0.463 ±0.086,1.696 ±0.475,ICD:0.284 ± 0.071;NIC,curve slope of the venous phase and ICD of the benign group:NICap:0.123 ±0.062,NICvp:0.290 ± 0.119,1.169 ±0.582,ICD:0.166 ±0.073,),but there was no significant difference between the patients with benign and malignant lesions in curve slope of the arterial phase.Conclusion Energy spectrum CT dual -phase enhanced scan can differentiate the nature of benign and malignant pulmonary occupying lesions,and has certain clinical application value.

Clinical Efficacy of BCG-polysaccharide Nucleic Acid Cominbed with Isotretinoin in the Treatment of Pa-tients with Plane Warts / 中国药师

Objective:To study the clinical efficacy and safety of BCG-polysaccharide nucleic acid combined with isotretinoin soft capsules in the treatment of plane warts. Methods:Seventy patients with plane warts were randomly divided into the observation group and the control group(n=35). The control group were treated with isotretinoin soft capsules, limp 10mg, bid, 4 weeks to 10mg, qd. The observation group were given BCG-polysaccharide nucleic acid 1ml, im, every otherday, on the basis of the contrl group. After 8 weeks of treatment, T cell populations and immunoglobulin levels were detected, and the efficacy and adverse reactions were recorded. Results:After the treatment, T cell population level, IgG and IgA of the two groups were significantly improved(P0. 05). Conclusion:The clinical efficacy of BCG-polysaccharide nucleic acid combined with isotretinoin soft capsules in the treatment of plane warts is sig-nificant with promising security.

Evaluation on the clinical effects for asynchronous cerebral arterial thrombosis with treatment of cerebral circulation therapeutic equipment / 中国医学装备

Objective: To investigate the effect about the treatment in asynchronous cerebral arterial thrombosis of cerebral circulation therapeutic equipment with disease function rehabilitation and the trigger factor of stroke and cerebral blood flow in 108 cases. Methods:Treatment group on the basis of conventional drug treatment plus brain circulation function therapeutic apparatus (KJ-3000-a) treatment in patients with stroke in different disease period. Using the neural function defect score and controllable risk factors of stroke and TCD determination of internal carotid artery and vertebral artery blood flow changes in evaluation of cerebral circulation function therapeutic equipment therapy in treatment group and control group is effective and feasible. Results:Different stages of stroke treatment group after treatment of acute phase of the neural function defect score in the control group (t=-13.26, P<0.05), and factors caused troke such as blood pressure and cholesterol leels also declined significantly than control group (t=7.39, t=7.75, P<0.05), cerebral blood flow between treatment group and the control group after treatment mainly reflected in the increased blood flow of part of left and right internal carotid artery and Vertebral Artery ,as Vd, Vs and Vm. Conclusion:Against the illness severity stroke with drug therapy on the basis of brain circulation function therapeutic instrument to stimulate rehabilitation treatment is convenient, economic, safe, effective, can be applied to stroke periods with high blood pressure, cholesterol levels, but acute stage used the best, after post-stroke patients vital center smoothly, can take early used cerebral circulation function therapeutic equipment for rehabilitation.

Efficacy analysis of endovascular graft exclusion for the treatment of 24 patients with infra-kidney abdominal aortic aneurysms / 中国医师进修杂志

Objective To investigate the efficacy and the indication and the management of perioperative complications in treatment of infra- kidney abdominal aortic aneurysm (AAA) by using endovascular graft exclusion (EVGE). Methods From April 2006 to September 2008, 24 patients with infra- kidney abdominal aortic aneurysms were diagnosed by contrast-enhanced CT or MRI scan. Vascular access was obtained through the bilateral femoral artery after arteriotomy and stent-graft was deployed into AAA of below the renal artery to occlude the left over cavity of AAA. The stent- graft was extended and anchored to the both side wall of AAA, the blood flow enter into the arteria iliaca communis through the sten't.Results Stent-graft deployment was successfully performed in all the patients. Immediate aortography after the procedure showed no leakage in 20 patients and the type Ⅰ minor leakage in 4 patients. No stent movement or organ and both lower extremities ischemia was found at the early post operative stage in all the patients. Six months after the operation, in all the 24 patients, contrast-enhanced CT scan showed the disappearance of the aneurysm and thrombosis at the level of the stent. Conclusions EVGE is simple,minimally invasive,less complication and quick recovery after operation. Thus it becomes first choice for the treatment of AAA for the elder patients.

The study of graft offset in the anterior cruciate ligament reconstruction / 中华骨科杂志

Objective To investigate the impact of graft position shift on anterior cruciate ligament reconstruction induced by femoral fixation of interference screw. Methods Nineteen fresh cadaveric knees were used and assigned to three groups. 1) Study of graft position shift: 5 knees were randomly selected, interference screws of 7 mm, 8 mm and 9 mm were used in autologous tendon fixation, then the graft position shift were measured. 2) Study of isometry: 7 knees were randomly divided into the isometric reconstruction group (D group). In the D group, Retrobutton, interference screw and interference screw in location-corrected bone tunnel were used respectively as fixation. The isometry of grafts was evaluated. 3) Study of tibia anterior translation: 7 knees were randomly divided into the anatomic reconstruction group (J group). In the J group,the tibia anterior translation was measured in four different conditions in the same joint: intact knee joint,knee joint without ACL, ACL anatomic reconstruction by interference screw fixation, and ACL anatomic reconstruction by interference screw fixation with corrected bone tunnel location. Results 1) With 7 mm, 8mm and 9 mm interference screw fixation, graft position shift were (2.36±0.11) mm, (2.72±0.06) mm and (3.00±0.06) mm respectively. 2) Graft length change: graft length change in Retrobutton group and corrected bone tunnel group were less than 3 mm, while graft length change in those fixed with interference screw were stretched in more than 3 mm. 3) Study of tibia anterior translation: there was no difference among the intact group, the anatomic group and the corrected group at 0° and 15°. However, the difference was found between the intact group and other groups at 30°、60° and 90° of flexion, as well as between these two reconstructed methods at 20° joint flexion (P＜0.05). Conclusion In both isometric and anatomic ACL reconstruction with interference screw, the graft is pushed tightly toward the femoral tunnel wall, which shifts the graft away from the desired position. In our study we find out that the corrected location of the femoral bone tunnel significantly improves the isometry of ACL reconstruction and anatomic reconstruction.

Influence of nursing intervention on caesarean section rate in grassroots hospitals / 中国实用护理杂志

Objective To observe the effect of nursing intervention on caesarean section rate in grass-roots hospitals. Methods 240 parturient women who were to infanticipate were divided into the experimental group and the control group with 120 patients in each group according to hospitalization sequence. Parturient women in the experimental group received nursing intervention with new nursing model during the delivery, while women in the control group just finished parturition using routine procedures. The caesarean section rate of the two groups were compared with χ2 test. Results The caesarean section rate of parturient women in the experimental group was significantly lower than that in the control group. Conclusions Nursing inter-vention has certain effect on decreasing the caesarean section rate in grassroots hospitals.

Thinking and look forward to clinical study of allergic rhinitis by treatment of traditional Chinese medicine / 中国中药杂志

Allergic rhinitis (AR) is one kind of nose mucous membrane inflammation reactiveness disease, which is mediated by IgE for the environment sensitizin. The major clinical symptoms are: nose itch, the sneeze, nasal discharge, nasal obstruction and so on. It is thought that the acient disease named "Bi Qiu" has included AR. By comparing the AR clinical manifestation characteristic with trditional Chinese medicine (TCM) "Feng/wind" induced disease characteristic and the "Feng/wind" disease clinical manifestation characteristic, we can know that AR should belong to the "Feng/wind" disease category of TCM. It is shown that the "Feng/ wind" medicine is the most windly appeared in AR treatment formulas of TCM clinical research data (in each formular) in the past 10 years. To aim directly at three knids of problem in TCM clinical research, we discussed five problems which should pay close attention to the feature research, therefor to provide a mirror for AR clinical and experimental studies by TCM.

Sperm nuclear histone H2B: correlation with sperm DNA denaturation and DNA stainability / 亚洲男科学杂志(英文版)

<p><b>AIM</b>To examine the relationship between sperm DNA damage and sperm nuclear histone (H2B) staining.</p><p><b>METHODS</b>We evaluated sperm samples from 14 consecutive asthenoteratozoospermic infertile men and six consecutive fertile controls. Sperm nuclear histone (H2B) staining and sperm chromatin integrity (assessed by sperm chromatin structure assay and expressed using the percentage of (i) DNA fragmentation index [% DFI] and (ii) high DNA stainability [% HDS)]) were evaluated.</p><p><b>RESULTS</b>Histone H2B immunocytochemistry demonstrated two nuclear staining patterns: (i) focal punctate staining; and (ii) diffuse staining. Infertile men had a higher mean percentage of spermatozoa exhibiting diffuse H2B staining than did fertile men (7.7% +/- 4.6% vs. 1.6% +/- 1.2%, respectively, P < 0.01). We observed significant relationships between the proportion of spermatozoa with diffuse nuclear histone staining and both sperm % DFI (r = 0.63, P < 0.01) and sperm %HDS (r = 0.63, P < 0.01).</p><p><b>CONCLUSION</b>The data demonstrate that infertile men have a higher proportion of spermatozoa with diffuse histone H2B than do fertile men and suggest that sperm DNA damage might, at least in part, be due to abnormally high histone H2B levels.</p>

Intradiscal therapy with 10% hypertonic saline for lumbar disc herniation / 介入放射学杂志

Objective A clinical trial of intradiscal injection of hypertonic saline to treat lumbar disc herniation, and observation of its effect, complications and changes in intervertebral disc tissue were performed for clinical application. Methods Discography was performed under local anesthesia, followed by a single injection of 1.0～2.5 ml 10% hypertonic saline. Results 262 cases were included in the study, with 4 years of average follow up. The final outcome ranked excellent in 93 cases and good in 100 cases. As a whole, the effective rate was 75.7% and the improvement rate was 71.5%. The procedure was effective in protrusion and confined extrusion types. 13 cases (6.7%) relapsed after the injection. No complication occurred. 26 cases resorted to surgery because of failure in intradiscal therapy, but no change was found, in the nerve and surrounding tissue of the intervertebral disc attributable to the injection during the surgical operation. The main causes of failure were the marked protrusion or non-contained extrusion, or the combination with spinal canal stenosis. Changes in the nucleus pulposus shown microscopically were similar to those resulted by the action of enzyme injection, but the injury to the disc was much milder.Conclusions Intradiscal therapy with 10% hypertonic saline for lumbar disc herniation is effective, mini-traumatic and safe.