ABSTRACT
Objective To establish the determination method of Codeine phosphate in Qiangli Pipa Syrup by RP-HPLC.Methods Using XDB-C18 Eclipse column (250 mm × 4.6 mm,5 ìm),acetonitrile:0.035 mol/L sodium acetate solution (glacial acetic acid to adjust pH =3.5) (25:75) as mobile phase,the flow rate was 1.0 mL/min,the detection wavelength is 240 nm,the column temperature is 40.Results the linear range of codeine phosphate in 2.024-40.24 ìg/mL was goody =8.18 × 105x + 12.2 (r =0.999 2);the average recovery rate was 98.2％ and RSD was 1.0％ (n =6).Conclusion This method is simple,rapid,accurate,and reliable,with high sensitivity,and could be applied to determination of Qiangli Pipa Syrup codeine hydrochloride content and controlment of the drug quality.
ABSTRACT
Objective:To investigate the correlation between the component contents and near-infrared spectral characteristics of sophora pieces from different habitats to confirm the near-infrared spectral features of sophora pieces. Methods: The near-infrared spectroscopy of the sophora extract was determined to confirm the characteristic absorption of principal components. Meanwhile, the content of main ingredients in sophora pieces was measured by HPLC, and the near-infrared spectral characteristics of sophora pieces from different places were compared analyzed. Results: The near-infrared spectral features of sophora pieces from different habitats showed some differences with something in common. There was a close relationship between the near-infrared spectral features and the compositions and the main ingredients. Conclusion:Combining with the near-infrared spectra of sophora extract can effectively identify the characteristics of sophora pieces from different places, which is beneficial to the establishment of a reasonable and effective near-in-frared qualitative analysis model.