ABSTRACT
Objective @#To develop a high modulus and high strength biodegradable silk fibroin GBR membrane to address the issue of maintaining the space for bone regeneration in the repair of osseous defects . @*Methods @#After purifying silk fibroin protein , membrane materials were prepared using evaporation-hot pressing method . The physi- cal and chemical properties and biological performance of the membranes were evaluated using stretching tests , in vitro simulations , and cell co-culturing methods . @*Results @#A silk fibroin GBR membrane was successfully fabrica- ted , resulting in a simulated degradation rate of 35 . 3% after 12 h in vitro . The wet-state elastic modulus reached 45 MPa , while the tensile strength reached 8. 39 MPa. Furthermore , the cell survival rate was nearly 100% after 7 days . @*Conclusion @#The biodegradable GBR membrane produced in this study possesses high modulus and strength , as well as excellent biocompatibility , offering a promise as a foundation for addressing the bone defect re- pair and bone space maintenance .
ABSTRACT
Oral squamous cell carcinoma (OSCC) is one of the most common cancers worldwide, and with 354 864 new cases each year. Cancer metastasis, recurrence, and drug resistance are the main causes to cripples and deaths of OSCC patients. As potent growth factors, fibroblast growth factors (FGFs) are frequently susceptible to being hijacked by cancer cells. In this study, we show that FGF8 is upregulated in OSCC tissues and high FGF8 expression is related with a set of clinicopathologic parameters, including age, drinking, and survival time. FGF8 treatment enhances the invasive capability of OSCC cells. Lentivirus-based FGF8 expression promotes OSCC metastasis in a mouse lung metastasis model. Further, mechanistic study demonstrates that FGF8 induces epithelial-mesenchymal transition (EMT) in OSCC cells. These results highlight a pro-metastatic function of FGF8, and underscore the role of FGF8 in OSCC development.