ABSTRACT
ObjectiveTo observe the protection of Baweichenxiang powder on cerebral ischemia-reperfusion injury in rats.MethodsSD rats were randomly divided into six groups,namely,sham operation group,model group,nimodipine group,Baweichenxiang powder high,medium and low-dose group.Cerebral ischemia-reperfusion rat model was established by gavage.Observe the effects of different doses of Baweichenxiang powder on behavior in rats,brain water content,infarct size,SOD,MDA and morphological changes.ResultsCompared with the control group,Baweichenxiang powder significantly reduced behavioral score (P＜0.05),reduced brain edema,decreased infarct size,improved brainmorphological changes,increased SOD activity(P＜0.05,P＜0.01)and reduced the MDA content(P＜0.05).ConclusionBaweichenxiang powder of high dose and middle dose have significant protective effect for cerebral ischemia,the mechanism may be related to its improving levels of free radicals and lipid peroxidation.
ABSTRACT
Objective To study the effects of anti-oncogene WWOX on cell growth of epithelial ovarian cancer,in order to find a new approach of gene therapy for ovarian cancer.Methods A eukaryotic expression vector containing WWOX was transfected into ovarian cancer cell line HO8910 in vitro (recombinant plasmid group),and positive cell clones were selected and amplified.Expression of WWOX protein was detected by western blot. Untransfected cell(blank contrast group) and transfected empty plasmid cell(empty plasmid group)were served as control groups.In vitro,the biology effect of WWOX on HO8910 cell was analyzed throush the methyl thiazolyl tetrazolium test,transwell chamber cell invasion assay in vitro,agarose clony-formation and flow cytometry.In vivo,the cell of transfection was transplanted intraperitoneally in to BALB/c nude mice.The survival time and growth ability of nude mice were observed.Results (1)Recombinant plasmid group cell could steadily express WWOX protein,while in empty plasmid group and blank control group the expression of WWOX protein were not detected.(2)The growth rate of recombinant plasmid group cell was inhibited.(3)The agnrose clony-formation rate of recombinant plasmid group(19.8%)was significantly lower than that of the empty plasmid group(54.5%)and blank control group(56.0%,P＜0.05).(4)Flow cytometry showed that(72.08±0.39)% of cells was arrested at G0/G1 stage in recombinant plasmid group, while in empty plasmid group and blank control group G0/G1 stage cells were at (41.02±1.08)% and (39.31±0.67)% (P＜0.05). (5) In vitro invasion assay showed that invasion cell number in recombinant plasmid group (89.7±3. 1 ) was not significantly different from that of empty plasmid group(91.2±1.3) and blank control group(91.4±1.3, P ＞0. 05). (6) In vivo test in nude mice showed that WWOX gene could inhibit tumor growth of the HO8910 cells. Conclusions Tumor suppressor gene WWOX could interfere with the cell cycles of ovarian cancer cell and inhibit cell proliferation. As a new valuable tool,it premises to have application in the gene therapy of ovarian cancer.
ABSTRACT
PBL is problem-based and student-centered learning pattern. PBL has been proved to be an effective teaching,which is helpful to improving the students’learning enthusiasm and learning efficiency,and to training their clinic thoughts and the capability of teamwork.
ABSTRACT
Objective To assess the clinical significance of changes tumor necrosis factor(TNF)-alpha level and the relationship between serum TNF-? and glucose levels in patients with gestational diabetes mellitus(GDM). Methods 71 patients with gestational diabetes mellitus were enrolled.40 normal pregnant women were assigned to be control group.Then the concentrations of blood glucose and TNF-? were determined. Results Blood TNF-? level in GDM patients was signficantly higher than that in normal pregnant women(P
ABSTRACT
0 05). In high grade squamous intraepithelial lesion (HGSIL), the differences between the laser treated group and either LLETZ or total hysterectomy groups turned into normal rate was significantly (P0 05).Conclusions Expectant treatment for CIN I is safe in a reliable patient population. LLETZ is a safe and effective treatment for HGSIL. The long-term efficacy of LLETZ should be investigated and assessed by extending the period of follow-up.