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Objective To study the effect of multiple IPL treatment on cell senescence markers of skin fibroblasts using UVA as a control and to make clear whether the multiple IPL treatment may result in cell senescence.Methods Cells were divided into three groups: one group without irradiation as a control,one group receiving IPL treatment with 15 J per cm2,and the last group receiving UVA irradiation with 9 J per cm2.IPL and UVA irradiation were performed once a day during five days.On the sixth day,the cells were collected.Senescence-associated β-galactosidase (SA-β-Gal) staining,cell cycle,reactive oxygen species (ROS) and telomere length were determined.Results Our results showed that five consecutive days of IPL irradiation had no effect on the activity of SA-β-Gal and telomere length and decreased the G1 ％ of cell cycle and the level of ROS in comparison with the control group (P＜0.05).On the contrary,five consecutive days of UVA irradiation increased the activity of SA β Gal and the level of ROS,shortened the length of telomere and no obvious change in the G1 ％ of cell cycle in comparison with the control group.Conclusions Multiple UVA irradiations induce cell senescence.On the contrary,multiple IPL treatments could not induce cell senescence.
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Objective To observe the improvement in manifestation of port wine stains (PWS) with photodynamic therapy (PDT),and to further evaluate the safety and efficacy of PDT in treatment of PWS.Methods A total of 125 patients with PWS were treated with PDT.The lesion of PWS was exposured to the copper vapor laser locally after injection of photosensitizer PSD-007 based on the weight of body via vein.Before each session,lesions were photographed,and therapeutic effects were evaluated by physicians based on improvement in color,thickness,and lesion area recorded on the photos.Return visit was carried out after 2 months.The improvement rate was classified as 5 grades to value the therapeutic effect of last treatment.In addition,patients satisfaction was also investigated.The treatment was determined to terminate or continue depending on the change of red spot including color fading and flatting.Results Patients with PWS responsed remarkably to the PDT.The healing rate was 49.60 ％ and excellence rate 96.00 ％ after less than 4 session treatments.Among the total of healing patients,13.60 ％00 were released from PWS with no more than 2 courses of treat ment.The satisfactory rate of patients was 95.20 ％ during observation.Conclusions PDT is a safe and highly effective treatment for PWS with good cosmetic results in the dermatologic clinic.
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Objective To study the protective effects of intense pulse light (IPL) on the injury of normal human skin fibroblasts (FB) induced by ultraviolet A (UVA Ⅰ ) in vitro and to explore its possible mechanism. Methods The human skin fibroblasts were isolated and cultured, and then irradiated by UVA Ⅰ (9 J/cm2) and IPL (15 J/cm2), respectively. The proliferative ability of the cells were detected by CCK-8. Cell cycle was detected by flow cytometry, and cylin D1 and CDK2 protein expression levels were detected by Western blot. Results Different doses of UVA Ⅰ irradiation caused certain damages of cultured fibroblasts. With the increasing of of UVA Ⅰ dose, cell proliferation was decreased. Cells went to death at the exposure to 11 J/cm2 UVA Ⅰ , while the proliferative activity did not change much at 7 J/cm2 UVA Ⅰ . Cells were treated with UVA Ⅰ for other 2 days, then with IPL irradiation for other 2days, showing clear stimulating to the cell proliferation as compared with the cells that received UVA Ⅰ treatment only. Flow cytometry results showed that an increase of cell proliferating index, and cell cycle protein cyclin D1 and CDK2 expression levels were also upregulated after IPL irradiation.Conclusion UVA Ⅰ irradiation may cause cell damage as showed by cell growth index, cyclin D1 and CDK2 expression, and this injury could be protected partly by IPL treatment. The intense pulsed light may regulate the expression of cyclin proteins that may promote normal fibroblast proliferation, which could be one of the mechanisms of IPL skin rejuvenation.
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Objective To assess the efficacy and safety of intensive pulse light (IPL) device for facial rejuvenation and the treatment of hyperpigmented lesions, facial telangiectasias, acne vulgaris and hair removal. Methods One hundred females who claim to improve their skin texture, hair re-moval and patients with hyperpigmented lesions, facial telangiectasias and acne vulgaris were treated with IPL device. Patients received five treatments with the time interval of 3 weeks to 1 month. Pho-tographs were assessed 1 month after the last treatment. Results For facial skin texture, the total im-provement were scored 100 %. For hyperpigmented lesions and facial telangiectasias, the total im-provement reached to 90%. For ache vulgaris, the total improvement reached to 75 %. For hair re-moval, the total improvement was 95 %. Conclusion The IPL device is an effective and safe modality for the improvement of skin texture, hyperpigmented lesions, facial telangiectasias and hair removal, and a novel modality for the treatment of acne vulgaris.