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Objective To investigate the difference of depressive symptoms between adolescents and adults,and to provide possible basis for early detection of adolescent depression.Methods From July 2021 to June 2022,a total of 4 096 patients with"depression"in the psychiatric clinic of the First Affiliated Hospital of Chongqing Medical University were selected as the research objects.They were divided into the adolescent group(n=2 439)and adult group(n=1 657)according to their ages,and the results of self-rating depression scale(SDS)and symptom checklist 90(SCL-90)were collected and analyzed.Results There were significant differences in nationality,residence,native place,family history and degree of depression between the two groups(P<0.05).The adolescent group has more severe depressive symptoms,which were mainly manifes-ted in negative ideas,obsessive-compulsive symptoms,hostile and interpersonal relationship,and psychotic symptoms(P<0.05).The adult group showed more obvious in sleep(P<0.05).Conclusion Early inter-vention should be carried out for adolescents'depressive symptoms such as negative thoughts.
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Improving the reproducibility of biomedical research results is a major challenge. Transparent and accurate reporting of the research process enables readers to evaluate the reliability of the research results and further explore the experiment by repeating it or building upon its findings. The ARRIVE 2.0 guidelines, released in 2019 by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), provide a checklist that is applicable to any in vivo animal research report. These guidelines aim to improve the standardization of experimental design, implementation, and reporting, as well as enhance the reliability, repeatability, and clinical translation of animal experimental results. The use of the ARRIVE 2.0 guidelines not only enriches the details of animal experimental research reports, ensuring that information on animal experimental results is fully evaluated and utilized, but also enables readers to understand the content expressed by the author accurately and clearly, promoting the transparency and completeness of the fundamental research review process. At present, the ARRIVE 2.0 guidelines have been widely adopted by international biomedical journals. This article is based on the best practices following the ARRIVE 2.0 guidelines in international journals, and it interprets, explains, and elaborates in Chinese the fifth part of the comprehensive version of the ARRIVE 2.0 guidelines published in PLoS Biology in 2020 (the original text can be found at https://arriveguidelines.org). This section includes the items 6-11 of Recommended 11 section, covering "Animal Care and Monitoring", "Interpretation/Scientific Implications", "Generalisability/Translation", "Protocol Registration", "Data Access" and "Declaration of Interests". Its aim is to promote a comprehensive understanding and use of the ARRIVE 2.0 guidelines among domestic researchers, to enhance the standardization of experimental animal research and reporting, and to promote high-quality development of experimental animal sciences and comparative medicine research in China.
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Improving the reproducibility of biomedical research results is a major challenge.Transparent and accurate reporting of the research process enables readers to evaluate the reliability of the research results and further explore the experiment by repeating it or building upon its findings. The ARRIVE 2.0 guidelines, released in 2019 by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), provide a checklist applicable to any in vivo animal research report. These guidelines aim to improve the standardization of experimental design, implementation, and reporting, as well as the reliability, repeatability, and clinical translatability of animal experimental results. The use of ARRIVE 2.0 guidelines not only enriches the details of animal experimental research reports, ensuring that information on animal experimental results is fully evaluated and utilized, but also enables readers to understand the content expressed by the author accurately and clearly, promoting the transparency and integrity of the fundamental research review process. At present, the ARRIVE 2.0 guidelines have been widely adopted by international biomedical journals. This article is a Chinese translation based on the best practices of international journals following the ARRIVE 2.0 guidelines in international journals, specifically for the complete interpretation of the ARRIVE 2.0 guidelines published in the PLoS Biology journal in 2020 (original text can be found at https://arriveguidelines.org). The fourth part of the article includes the items 1-5 of ARRIVE 2.0 Recommended 11 section, which covers "Abstract" "Background" "Objectives" "Ethical statement" and "Housing and husbandry". Its aim is to promote the full understanding and use of the ARRIVE 2.0 guidelines by domestic researchers, enhance the standardization of experimental animal research and reporting, and promote the high-quality development of experimental animal technology and comparative medicine research in China.
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Objective To explore the effect evaluation of choking risk assessment and intervention on the choking prevention of homeless inpatients with psychiatricc. Methods Totally 163 homeless inpatients with psychiatric disorder were selected from July 2012 to June 2016, 56 cases from July 2012 to December 2013 were assigned to the control group, another 107 cases from January 2014 to June 2016 was set as the intervention group. The control group received routine nursing safety management, on the basis of it, the intervention group received choking risk assessment through choking risk assessment scales. To take choking risk intervention on the patients of reached choking danger threshold. The incidence of choking and choking ending were compared between the two groups and the choking risk levels and scores in the intervention group before and after intervention were also compared. Results The incidence of choking in the intervention group and the control group was 0.93%(1/107)and 10.71%(6/56)respectively. Choking ending: 2 heal, 3 aspiration pneumonia, 1 death of 6 cases in the control group and 1 case was cured, no death and aspiration pneumonia occurred in the intervention group, the statistical differences were significant (χ2=9.399,18.554, P < 0.01 or 0.05). There were 28.0% (30/107) reached level Ⅰ, Ⅱ and Ⅲ choking threshold in the intervention group and rank mean of choking risk scores were 41.29. After interventions were 8.4% (9/107) and 25.50 respectively, the statistical differences were significant (χ2=10.231, P<0.05;Z=7.511, P<0.01). Conclusions The corresponding nursing intervention on the patients of choking risk can reduce and avoid choking accidents and protect the safety of homeless inpatients with psychiatric disorder effectively and improve the quality of care.
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Infectious bursal disease virus (IBDV) VP4 plays an important role in immunosuppression of host. In order to develop monoclonal antibodies (McAbs) against VP4, we vaccinated BALB/c mice with His-VP4, screened and subcloned positive clones. We established 4 hybridoma cell lines that stably secreted McAbs against VP4 and named these cell lines 3B3, 3H11, 4C8 and 4G6, respectively. We tested the dissociation constant (Kd) of these McAbs, and found that their K(d)s were 4.61 x 10(-11), 1.71 x 10(-10), 4.26 x 10(-11), 5.02 x 10(-11), respectively. The isotypes of these McAbs were determined to be IgG1, IgG1, IgG2b and IgG1. These McAbs specifically bound to VP4 in IBDV infected DF-1 cells as demonstrated by Western blotting analysis and fluorescence antibody assay. These McAbs would help to detect IBDV infection and to analyze the biological activities of IBDV VP4.
Subject(s)
Animals , Mice , Antibodies, Monoclonal , Blotting, Western , Cell Line , Fluorescent Antibody Technique , Hybridomas , Infectious bursal disease virus , Mice, Inbred BALB C , Viral Structural Proteins , Allergy and ImmunologyABSTRACT
<p><b>OBJECTIVE</b>To determine the prevalence of occupational musculoskeletal disorders (OMSD) and its risk factors among workers in three manufacturing industries in Zhongshan, China by cross-sectional epidemiological investigation.</p><p><b>METHODS</b>A total of 2 035 workers from the industries of metals (1001 persons), electrical appliances (455 persons), and furniture (579 persons), including 1 402 males and 633 females, were selected; the mean age was 32.9 ± 8.2 years, and the mean length of service was 6.4 ± 5.6 years. A revised Northern Europe Standardized Questionnaire was used for cross-sectional epidemiological investigation of OMSD.</p><p><b>RESULTS</b>The results showed that OMSD in these workers was primarily located in the neck, waist, and shoulder, with annual prevalence rates of 23.1%, 20.1%, and 15.8%, respectively. The overall prevalence of OMSD was 43.1% in metal industry, 44.0% in electrical appliance industry, and 26.6% in furniture industry. OMSD prevalence showed significant differences between different industries (χ(2) = 54.2, P < 0.01). The prevalence of OMSD in the shoulder and back increased with working years (P < 0.05). Logistic regression analysis showed that the risk factors for OMSD were working age >10 years, safety behavior such as "bending down when lifting heavy things from the ground", and different types of industries.</p><p><b>CONCLUSION</b>OMSD is mainly manifested by neck pain, waist pain, and shoulder pain among front-line manufacturing workers in Zhongshan, and working age, poor labor posture, and different types of industries were risk factors for waist pain.</p>