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Objective To study liquid based cytology test and its application values in the detection of fiber bronchoscopic aspi‐rates .Methods LBP sedimentation type of ThinPrep cell production system combined with the conventional smear were used to de‐tect 2000 specimens of fiber bronchoscopic aspirates ,and conduct microscope observation and cytological diagnosis .Results Based on liquid based cytology cell production method ,there were 614 positive cases (30 .7% ) ,267 cases of squamous cell carcinoma (13 .4% ) ,and 217 cases of adenocarcinoma(10 .9% ) ,83 cases of small cell carcinoma (4 .2% ) ,and 47 cases of undifferentiated car‐cinoma (2 .4% ) .The diagnostic sensitivity for lung cancer of liquid‐based cytology test was 64 .6% and conventional smear was 49 .2% .We also found 7 cases of fungus positive ,35 cases of typical caseous necrosis with liquid based cytology test .Conclusion The ThinPrep cell production has a more clean background ,more intact cell structure ,more efficient observation and increased posi‐tive rate of detection of cancer cells than the smears made by traditional methods .A combination of two methods can increase the accuracy of cytological detection of fiber bronchoscope aspirates and positive detection rate ,and also can assist the diagnosis of fungi and TB infections .
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Objectives To study if there exists the ununi formity of drug resistance of mycobacterium tuberculosis (M.TB) isolated from MDR-TB patients .Methods One MDR-TB patient was chosen for this study a nd his sputum was cultured.The suspension of the culture was filtered through 8 0?m -pore-size membrane filter and single M.TB suspension was obtained.The single M.TB suspension was cultured on Lowsenstein Jensen (L-J) medium.24 of col onies were obtained and they were cultured respectively again to get pure strain s.The drug sensitivity test was carried out for these 24 pure strains of M.TB wi th Chinese standard method.H 37 Rv was taken as control.Results 1 strain (4 2%) was found to be susceptive to INH,22 strains ( 91 6%) resistant to low concentration INH (H 1 ),1 strain (4 2%) resistant to high concentration INH (H 10 ).2 strains (8 3%) were found t o be susceptive to RFP,2 strains (8 3%) resistant to low concentration RFP(R 50 ),20 strains (83 4%) resistant to high concentration RFP(R 250 ).We f ound 1 strain (4 2%) susceptive to SM,23 strains (95 8%) resistant to high concentr ation SM(S 100 ).All were susceptive to EMB.Conclusions The ununiformity of drug resistance exists among the M.TB isolated from the MDR-TB patients.Sensitive and resistant strains exist together,low and high concentration-drug-resistant strains coexi st in the same situation.The majority of M.TB is drug-resistant and only a few are still sensitive to low concentration- drug- resistant.
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Aim To study the immunoregulatory effect of Mycobacterium vaccae vaccine on asthmatic guinea pig.Methods 30 guinea pigs were randomly divided into NS,asthma and Mycobacterium vaccae vaccine groups. A guinea pig model of asthma was formed with ovalbumin.Every guinea pig of Mycobacterium vaccae vaccine group was intramuscularly injected with 22.5 ?g Mycobacterium vaccae vaccine 10 days before ovalbumin immunization.mRNA expressions of IL-4,IL-5 and IFN-? in lung tissue,total IgE and ovalbumin-specific IgE in serum were determined. Results For Mycobacterium vaccae vaccine group guinea pigs, mRNA expressions(optical density value) of IL-4(0.060?0.018) and IL-5(0.052?0.006)in lung tissue were significantly lower than those of asthma group(0.111?0.025,0.106?0.030 respectively; both P