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Background: Developing the natural medicine that allow for the specific targeting cytotoxicity is a very important research area in the development of anti-tumor drugs. Aims and Objectives: This study was conducted to determine the targeted inhibitory effects of luteolin-loaded Her-2-poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) on gastric cancer cells and to delineate the mechanism underlying the inhibition of tumors by luteolin. Materials and Methods: Luteolin-loaded Her-2-PLGA NPs (Her-2-NPs) were prepared, physically and chemically characterized, and their effects on gastric cancer cells were investigated. The rate of NP uptake by cells and the cell morphology were observed using confocal microscopy; the rates of cell proliferation and apoptosis were identified using the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay and flow cytometry, respectively; and the mRNA and protein expression levels of forkhead box protein O1 (FOXO1) were determined using quantitative polymerase chain reaction and Western blotting, respectively. Results: Compared with nontargeted microspheres, Her-2-NPs led to significantly enhanced uptake of luteolin by SGC-7901 cells. Luteolin-loaded Her-2-NPs also significantly inhibited the proliferation of gastric cancer cells, weakened their migratory ability, and increased both the mRNA and protein expression levels of FOXO1. Conclusion: Luteolin-loading of Her-2-NPs could potentially be used as a novel anti-cancer drugs for targeted cancer therapy.
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Objective:To investigate the expression of STEAP1 in human breast cancer and its clinical significance. Methods:Immuno-histochemical staining was performed to detect the expression of STEAP1 in breast tissues from 40 normal women, 52 patients with breast fibroadenoma, and 211 patients with breast cancer in Qianfo Hill Hospital of Shandong Province from January 2007 to Decem-ber 2016. According to the expression level of STEAP1, the breast cancer patients were assigned into high and low expression groups. Clinical pathological characterization and Kaplan-Meier survival analysis of the two groups were performed. RT-PCR and Western blot were conducted to measure the expression of STEAP1 in mammary gland cells. The Oncomine and Kaplan-Meier Plotter databases were used to analyze the expression and prognosis of STEAP1. Results:Immunohistochemistry showed that the high expression rate of STEAP1 in breast cancer was 11.8%(25/211), which was significantly lower (P<0.001) than that in normal breast (85.0%, 34/40) and fi-broadenoma (84.6%, 44/52). The result was consistent with the Oncomine database analysis that the expression in breast cancer tis-sue was lower than that in normal breast tissue. RT-PCR and Western blot analyses showed that the mRNA and protein expression lev-els of STEAP1 were significantly higher in mammary epithelial cells than in MDA-MB-468, BT549 and MCF-7 breast cancer cell lines, and a significant difference was found among the four types of cells (P<0.001). Kaplan-Meier survival analysis and Kaplan-Meier Plot-ter database analysis showed that patients with high STEAP1 expression had better prognosis than those with low STEAP1 expression (P<0.05). Conclusion:The expression level of STEAP1 in normal breast was higher than that in breast cancer. High STEAP1 expression is associated with a better prognosis.
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The analysis of individual gene product should enable to clarify the role of a particular enzyme in a complex xylanase system of A. niger. The two genes encoding precursors of co-produced endo-1,4-¥â-D-xylanases, xynA1 and xynB, were isolated from Aspergillus niger SCTCC 400264 (SCTCC, China) by using RT-PCR technique and then successfully expressed in Escherichia coli BL21. The nucleotide sequences of the xynA1 and xynB genes revealed that they were only 52.5 percent homology to each other. Characterization of the recombinant enzymes revealed the different properties: the specific activity of recombinant XYNA1 was 16.58 U/mg compared to 1201.7 U/mg for recombinant XYNB; The optimum temperature and pH of the recombinant XYNA1 were 35 ¨¬C and 3.0, respectively, whereas the corresponding values for the recombinant XYNB were 55 ¨¬C and 5.0, respectively; The recombinant XYNB showed much more thermostability than recombinant XYNA1; The recombinant XYNB showed 94 percent of maximal activity after incubating in water for 60 min at 60 ¨¬C compared to no activity for recombinant XYNA1. Various metal ions had different effects on activity between the two recombinant xylanases.
Subject(s)
Aspergillus niger/isolation & purification , Aspergillus niger/pathogenicity , Base Sequence , Gene Expression , In Vitro Techniques , Polymerase Chain Reaction , Xylans/isolation & purification , Enzyme Activation , Methods , Methods , VirulenceABSTRACT
<p><b>OBJECTIVE</b>The paper was to evaluate the quality and characteristics of the Chinese Journal of Plastic Surgery(CJPS).</p><p><b>METHODS</b>With the database of Chinese Citation Index, the citation analysis was used to analyze the distribution of the cited original articles published in the CJPS from 2001 to 2005.</p><p><b>RESULTS</b>1045 papers were published during the period, and there were 530 (50.72%) cited by other researchers. The total frequency of citation was 1598, and each original article was averagely cited 3.02 times by other researchers. The published papers were cited by 289 journals and self-citing rate was 7.57%. The distribution of the most frequently cited authors covered 25 provinces, with Beijing, Shanghai, Shanxi and Guangdong Province in the lead in research work relevant to plastic medicine.</p><p><b>CONCLUSIONS</b>The Chinese Journal of Plastic Surgery has published high quality articles in the past five years. It is one of the most important information resource for the Plastic Surgery researchers and of the most important medical journals.</p>
Subject(s)
Bibliometrics , Periodicals as Topic , Surgery, PlasticABSTRACT
<p><b>OBJECTIVE</b>To observe anti-HEV IgG response to vaccination of recombinant antigen fragments and evaluate its protection from Hepatitis E Virus infection in rhesus monkeys (Macaca mulatta).</p><p><b>METHODS</b>Twelve monkeys were divided into three groups and immunized respectively with three different recombinant antigens: namely Ag1 (carboxyl terminal 431 amino acids of ORF2), Ag2 (128aa fragment at the carboxyl terminal of ORF2), and Ag3 (full length ORF3 ligated with two ORF2 fragments encoded by 6743-7126nt and 6287-6404nt). The monkeys were challenged intravenously with fecal suspension from experimentally infected rhesus monkeys, and the other three monkeys served as the placebo group for challenge with HEV. The dynamic changes of the levels of ALT and anti-HEV IgG were examined. Pathological changes of liver tissue were observed by light microscope. Excretion of virus was detected by RT-nPCR.</p><p><b>RESULTS</b>Hepatic histopathology of two monkeys in the placebo group was consistent with acute viral hepatitis, and ALT was elevated 3-4 weeks after inoculated with virus, up to 10-20 times higher than normal level. The liver tissue of monkeys immunized with antigen kept normal, ALT in several monkeys elevated mildly, and anti-HEV IgG conversation occurred at 1-2 weeks after vaccination, with the titer reaching 1:12,800. The virus RNA could be detected by RT-nPCR from days 7 to 50 in monkeys of control group, and from days 7 to 21 in vaccinated monkeys after challenged with virus.</p><p><b>CONCLUSIONS</b>The recombinant antigens could induce the production of anti-HEV IgG, which protected rhesus monkeys from acute Hepatitis symptoms related to HEV infection.</p>
Subject(s)
Animals , Antigens, Viral , Allergy and Immunology , Hepatitis E , Hepatitis E virus , Allergy and Immunology , Immunoglobulin G , Allergy and Immunology , Macaca mulatta , RNA, Viral , Blood , Recombinant Proteins , Allergy and Immunology , Vaccination , Viral Hepatitis Vaccines , Allergy and ImmunologyABSTRACT
Objective To study the development and research status of infectious diseases influ- ence in China and the academic influence of Chinese Journal of Infectious Disease.Methods Utilizing the literature metrology means,mainly based on《Chinese Journal Full-text Network Database》and《Chinese Citation Network Database》,statistics and analyses on the Cited Papers of Chinese Journal of Infectious Disease in 2000 to 2004 were made.Results The citation rate of 787 papers from 30 issues was 65.9%,citation frequencies in total were 2946 and the highest citation frequency was 245,the av- erage citation frequency was 5.7,and the rate of cited fund articles with total cited papers was 37.6% the total citation frequencies raised in the recent years and the average of influence factors was 1.281. Conclusion The Chinese Journal of Infectious Disease represents the highest level core periodical of infectious diseases in China and is one of the most important information resources in this research demesne.