ABSTRACT
Objective To investigate the inhibitory effect of matrine on inflammation by regulating Th1/Th2 bal-ance in asthmatic rats and the underlying mechanism related to SOCS3.Methods Ovalbumin-sensitized rats were established as asthma model, Animals randomly divided into four groups, as follows: control ( without any treatment) , model group, treatment group A ( low-dose matrine treated asthma rats ) and treatment group B ( high-dose matrine treated asthma rats) .The eosinophil counting, goblet cells percentage, inflammatory cell in-filtration in rat lung were analyzed and scored by morphological examination .IL-4 and IFN-γlevel in BALF were determined by ELISA and IFN-γ/IL-4 ratio was further calculated.Furthermore, the expression of SOCS3 in mRNA and protein level were detected by qRT-PCR and Western blot, respectively.Results Eosinophil count and percentage, goblet cell percentage and inflammatory cell infiltration score were significantly lower than that in treatment group A and B as compare to model group ( P<0.05 ) .The group A exhibited a lower IFN-γlevel and a higher IL-4 level ( P<0.05 ) .IFN-γlevel in treatment group A and B were higher while IL-4 level were lower as compare to model group.Meanwhile, SOCS3 mRNA level in rat lung tissue was elevated in model group.Ma-trine treatment decreased SOCS3 expression in group A and B .Similar trend was found in SOCS3 protein level. Conclusions Matrine may exhibit antiinflammatory effect by inhibiting SOCS3 expression and correcting Th1/Th2 balance in asthmatic rats.
ABSTRACT
Objective To study the effect of a polysaccharide from cuscuta chinensis lam (PCCL) on antisenility and its mechanism. Methods Sixty Kunming mice were randomized into 6 groups. The three PCCL groups were administrated with PCCL of 100, 200, 400 mg?kg -1?d -1 orally, the positive control group with vitamin E of 200 mg?kg -1?d -1, the model group and control group with the same volume of control solution only. At the same time, the model group, the positive group and the three PCCL groups were subcutaneously injected of 5% D-gal at the dose of 0.5 ml at the nape, and the control with the same volume of saline solution. Seven weeks later, the MDA, SOD activity, GSH-PX activity in the liver and kidney of mice and lipofuscin (LF) in mouse brain were detected with the methods of TBA, Nitrate, DTNB and Sohal, respectively. The data were analyzed with SPSS software and the data between groups were analyzed with one-factor variance analysis. Results Thymus index and spleen index dropped, LF rose in brain, malondialdehyde (MDA) content rose and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) dropped in liver and kidney in senile mouse model. PCCL administration of 100, 200, 400 mg?kg -1?d -1 made thymus index and spleen index rising, LF dropping in brain, MDA content dropping,SOD and GSH-PX LF rising in liver and kidney of senile mouse model. Conclusion PCCL may postpone senility, which mechanism probably connected with rising immunity, eliminating oxygen free radicals and antilipoperoxidation.
ABSTRACT
Aim To study the synergistic effect and its mechanism of polysaccharide from Ligustrum Lucidum Ait(PLLA)and polysaccharide from Cuscuta Chinensis Lam (PCCL)on antisenility. Methods To observe the effect on immune organs and antioxidation systems of mice with senile model caused by D-galatose (D-gal) and meanwhile administered with PLLA∶PCCL 50 ∶50 mg or 50 mg ∶200 mg or 200 mg ∶50 mg or 200 mg ∶200 mg?kg -1?d -1 designed by orthogonal method. Results Thymus index and spleen index differently rose,MDA differently dropped,activities of SOD and GSH-PX differently ascended in liver and kidneys,LF differently descended in brain of mouse with senile model administered with 50 mg∶50 mg or 50 mg ∶200 mg or 200 mg ∶50 mg or 200 mg ∶200 mg?kg -1?d -1 PLLA ∶PCCL.The statistical analysis indicated that the combination of PLLA with PCCL yielded of notable or extremely notable reciprocal effect on the experimental index of antisenility. Conclusion The combination of PLLA with PCCL can yield notable synergistic effect on antisenility,the optimal combination of both drugs is PLLA ∶PCCL 50 mg ∶200 mg, its mechanism is probably connected with immunity inhacement,eliminati on of oxyen free radicals and active oxyen and antilipoperoxidation.