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OBJECTIVE： To prepare Melatonin solid lipid nanoparticles （MT-SLNs）， and to investigate in vitro transcutaneous absorption characteristics of them in isolated skin of mice. METHODS： Using melatonin （MT） as model drug， glycerin sorbate as oil phase， poloxamer 188 （F188） as emulsifier， MT-SLNs was prepared by melt-emulsion method. The morphology of MT-SLNs was observed by TEM； the particle size distribution of MT-SLNs was measured by laser particle size analyzer， and the infrared spectrum characteristics were measured by infrared spectroscopy. The entrapped efficiency and drug loading amount were determined by HPLC and ultrafiltration centrifugation method. The transcutaneous absorption characteristics in vitro of MT-SLNs and MT raw material were compared using Franz diffusion cells method. RESULTS： The formulation of MT-SLNs included 2.5 mg/mL MT raw material， 25 mg/mL glycerin sorbate and 1％F188. The obtained MT-SLNs were spherical， and no aggregation was observed. Average particle size was （67.88±0.17）nm， and polydispersity index was 0.188±0.001. The characteristic absorption peaks of N—H stretching and bending vibration were not found in infrared spectra， and the characteristic absorption peaks of C—H stretching vibration of benzene ring shifted red to 1 750 cm－1. The entrapped efficiency and drug loading amount of MT-SLNs were （87.54±5.31）％ and （8.42±0.78）％. The transcutaneous absorption behavior of MT-SLNs and MT raw material conformed to zero-order kinetics release rule. The steady-state transmission rate of MT-SLNs [（31.71+2.78） μg/（h·cm2）] was significantly higher than that of its raw material [（10.32±3.24） μg/（h·cm2）]， and its lag time [（0.17±0.01） h] was significantly shorter than that of its raw material [（1.57±0.37） h] （P＜0.01）. CONCLUSIONS： Prepared MT-SLNs have small particle size and distributed evenly， and can promote in vitro transcutaneous absorption.
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Dilated cardiomyopathy (DCM) is the most frequent pattern of non-ischemic cardiomyopathy with poor prognosis and high mortality.Studies in recent years found that non-coding small RNA molecules (miRNA) were closely related to the clinical course of DCM.The present article made a review on the expression pattern of miRNA and development of DCM study.
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Objective:To investigate the effect of atorvastatin on the blood lipid and uric acid levels of elderly patients with coronary heart disease complicated with diabetes mellitus.Methods:116 patients with coronary heart disease and diabetes were randomly divided into the control group and the experimental group,58 cases in each group.Both groups of patients were given blood glucose control,blood pressure and other symptomatic treatment.The control group was treated with Aspirin Enteric-coated Tablets 0.3～0.6 g/times,3 times/d,oral,clopidogrel tablets,2 tablets each time,1 time/d,oral,nitroglycerin,0.25～0.5 g/time,3 times/d,with service;the experimental group was given atorvastatin on the basis of control group,10～20 mg/time,1 time/d,treatment for 4 weeks.During the treatment,the dosage was timely adjusted according to the conditions of patients.The serum low density lipoprotein cholesterol (LDL-C),high density lipoprotein cholesterol (HDL-C),triglyceride (TG),total cholesterol (TC),uric acid (UA),glycosylated hemoglobin (HbA1c) levels before and after treatment and the clinical treatment efficiency were observed and compared between two groups.Results:Compared with before treatment,the serum LDL-C,TG,TC,UA,HbA1c levels were decreased ahter treatment in both groups of patients,the HDL-C level was increased (P＜0.05);compared with the control group,the serum LDL-C,TG,TC,UA,HbA1c levels were lower,HDL-C level was higher in the experimental group (P＜0.05);compared with the control group,the effective rate of clinical treatment of the experimental group were higher (P＜0.05).Conclusion:Atorvastatin could effectively reduce the blood glucose,blood lipid,uric acid levels of elderly patients with coronary heart disease complicated with diabetes.
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Objective To explore the effect of the three level storehouse system on ward drug management.Methods The three level storehouse system was established in our hospital,which includes drug demanding quantity of ward,delivering quantity of the drug storehouse,and management supervision of pharmacy.Result After implementing three level storehouse system, the incidence of medicine expiry,improper storage,drug reception,confused marking ,improper placement and incorrect drug administration were decreased(P<0.05).Conclusion The three level storehouse system could improve the quality of hospital drug management as well as ensure clinical medication safety.
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Objective To study the applied value of multi-slice spiral CT(MSCT)in pulmonary lesions.Methods 68 cases with massive hemoptysis caused by lung tumor or other reasons underwent 16-slice spiral CT scan including plain and contrast-enhanced scan.Volume reconstruction(VR),multiplanar reconstruction(MPR),maximum intensity projection(MIP)and curved planar reconstruction(CPR)were performed using original images.The origin,number and shape of vessels feeding lesions were observed.Results Among 68 cases(two failed),the blood supply of pulmonary lesions was from bronchial artery in 60 cases,from the intercostals artery in 8 cases,from the internal thoracic artery in 5 cases,from the left subclavian artery in 6 cases,from the right inferoir phrenic artery in 5 cases,from the celiac trunk artery in 3 case and from the right renal artery in 1 case.The blood supply was from single artery in 50 cases,double arteriae in 10 cases and three arteriae in 6 cases.The supply arteriae were vascular plexus in 55 cases,meandering in 8 cases,net in 2 cases and aneurysm formation in one cases.Conclusion MSCT can clearly display the vessels feeding pulmonary lesions.