Discussion on the status quo and solutions to the prevention and control of birth defects among primary obstetricians and gynecologists in the era of molecular genetic testing / 中华医学遗传学杂志

Birth defects are an important factor for the quality of newborn population. With the development of molecular genetic technology, an increasing number of genetic disorders leading to birth defects can now be detected. The lack of the knowledge for the basics and clinical applications of molecular genetic techniques have emerged as a shortcoming for primary care physicians who have formed the first tier prevention for birth defects. Currently, government has paid more attention to the above problems and formulated more training programs for primary obstetricians and gynecologists, e.g., "Prenatal Screening and Prenatal Diagnosis Post Training Program", "National Birth Defects Training Program", "National Primary Obstetrician Training Program". To some extent, such programs have met the urgent need for birth defect prevention in primary hospitals. But at the same time, some problems have also emerged. For instance, the knowledge for birth defects among primary obstetricians and gynecologists is poor, and there is lack of young personnel. This article has aimed to discuss the strategies to systematically improve the ability for preventing birth defects among primary care physicians by analyzing the obstacles and challenges for primary obstetricians and gynecologists in the era of molecular genetic testing.

Prostasin gene polymorphism at rs12597511 is associated with severe preeclampsia in Chinese Han women / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Preeclampsia, characterized by hypertension and proteinuria, is a multifactorial disease associated with shallow invasion of trophoblast cells and inadequate spiral artery remodeling. Trophoblast and tumor cells have similar invasion mechanism. Prostasin is closely related to tumor development, invasion and metastasis and influences blood pressure through activating epithelial sodium channel. The effect of prostasin on the pathogenesis of preeclampsia remains unclear. This study investigated the association of prostasin gene at rs12597511 with severe preeclampsia.</p><p><b>METHODS</b>A single nucleotide polymorphism, rs12597511, was tested with polymerase chain reaction and restrictionfragment length polymorphism analyses in 179 severe preeclampsia patients and 222 normal pregnant women.</p><p><b>RESULTS</b>The frequencies of TC + CC genotypes were significantly higher in severe preeclampsia group compared with in control group (the adjusted odds ratio was 2.030, 95% confidence interval 1.195-3.449, P = 0.009). The C allele of rs12597511 was present significantly more often among women with severe preeclampsia (P = 0.001). Genotyping analysis showed that the C allele of rs12597511 could confer a risk for severe preeclampsia.</p><p><b>CONCLUSION</b>The higher frequency of C allele of prostasin gene at rs12597511 is associated with severe preeclampsia.</p>

Optimization of the Formula of Puerarin Derivative Nanoparticles by Central Composite Design / 中国药房

OBJECTIVE:To optimize the formula technology of puerarin derivative 4ac-loaded nanoparticles by central composite design. METHODS:4ac nanoparticles were prepared using solvent evaporation method, with mean diameter, encapsulation rate and yield as dependent variables, multi-linear regression and binomial fit were performed on the 3 independent variables at different level:dosages of supporter lactic acid/ glycolic acid copolymer and surfactant polyvinyl alcohol, and the diameter of pinhole. The optimum experimental conditions were predicted by response surface method. RESULTS:The binomial fitting equations of indexes were all superior to their multi-linear regression equations, and the predictive values in the established mathematical model were in good conformity with the experimental values. CONCLUSION:Central composite design plus response surface method shows a good predictability in optimizing the formula technology of 4ac-loaded nanoparticles.