ABSTRACT
ObjectiveTo observe the expression of 67 - KD laminin receptor (67 - KD LN -R) in human laryngeal squamous carcinoma cells and to analyse the correlation between its expressionand clinicopathological characters. MethodsWe examined the expression of 67 - KD LN - R in 30primary laryngeal squamous carcinomas and in 3 cervical metastatic lymph nodes by the immunohistoche-mical SP method on paraffin- embedded sections. ResultsThe positive rate of 67 - KD LN - R inthe primary site of laryngeal squamous carcinomas was 56.7 % (17/30). The immunoreactivity in 3 cer-vical metastatic lymph nodes was consistent with that in primary tumors. There was a significant corre-lation between the level of 67 - KD LN - R and the degree of tumor differentiation, the level being high-er in poorly differentiated tumors ( P<0.05). The level of 67 - KD LN - R in laryngeal squamous car-cinomas with cervical lymph node metastases was higher than that in those without cervical lymph nodemetastases ( P <0.05).Conclusion67 - KD LN - R plays an important role in the differentiation,proliferation, invasiveness and metastasis of laryngeal squamous carcinoma.
ABSTRACT
According to the pharmacokinetic theory, the authors studied the accumulation of gentamycin in perilymph of guinea pigs by searching the drug concentration at various time with Fluorescene Polarization Immunoassay (FPIA). The results indicated that: (1) The drug concentration in perilymph increased with the augment of total doses administered and there was almost a linear correlation between them. (2) In animals of 7-day injectious group, the drug could be still detected during 72 hours after the last dose administration. The concentration was 1 37?0.95 ?g/ml which was close to the level of serum minimal inhibitory concentration (MIC). It suggested that the elimination of the drug from inner ear was too slow and evident accumulation of gentamycin was indeed. (3) The fact mentioned above implied that the drug ototoxities could still damage the ear even if the drug administered had ceased before. It is the reason by which we could explain the problem encountered in clinic, that is why in some patients the ototoxic syndrom may still presented or enhanced even though the treatment has been stopped a few days before.