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Objective:To investigate the expression of N-myc downstream regulated gene 2(NDRG2) in hippocampus of epileptic mice and its effect on glutamate and glucose uptake in astrocytes of mice.Methods:The epileptic mouse model was induced by lithium chloride and pilocarpine nitrate. The mice were sacrificed at 1 d, 7 d, 15 d and 6 weeks after model establishment and the brain tissues of hippocampus were taken. Western blot was used to detect the expression of NDRG2 protein in hippocampus.The primary astrocytes of wild-type, NDRG2 + /+ and NDRG2 -/- mice were cultured and the NDRG2 phenotype of astrocytes was identified after primary culture. Glutamate content in the supernatant of astrocyte culture was determined by glutamate assay kit and ultraviolet spectrophotometer. Flow cytometry was used to detect the positive rate of 2-NBDG fluorescently labeled astrocytes. Results:(1) Compared with the control group (0.25±0.07), the expression of NDRG2 in the hippocampus of mice increased significantly in the acute phase of epilepsy (1 d(0.45±0.06, t=-3.84, P<0.05), 7 d(0.54±0.09, t=-4.30, P<0.05), 15 d(1.04±0.06, t=-15.08, P<0.01)), and remained significantly high in the chronic phase of epilepsy( 6 weeks (1.30±0.16, t=-10.40, P<0.01)). (2) The content of residual glutamate in the supernatant fluid of primary cell culture medium was detected.It was found that the uptake of glutamate by astrocytes in the NDRG2 -/- group was significantly lower than that in the NDRG2 + /+ group ((689.03±101.78) μmol/L, (113.67±37.35) μmol/L; t=9.19, P<0.01). (3) Western blot results showed that the expression of EAAT1 protein in NDRG2 -/- primary astrocyte was significantly lower than that of NDRG2 + /+ primary astrocyte(0.34±0.03, 1.16±0.21), and the difference was statistically significant ( t=-6.59, P<0.01). (4) Flow cytometry results showed that the positive rate of astrocyte in NDRG2 -/- group cells was significantly lower than that in NDRG2 + /+ group cells ((17.60±5.72)%, (72.22±8.35)%), and the difference was statistically significant ( t=-13.22, P<0.01). Conclusion:NDGR2 is closely related to the occurrence and development of epileptic diseases. The expression of NDRG2 is beneficial to exert its physiological function of EAAT1 and promotes the uptake of glutamate and glucose by astrocyte. It may be a potential cell protective factor to promote nerve protection and repairment.
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Cellular and Molecular Basis of Medicine is a basic module course newly established by our school that is compulsory for medical students. This module course overthrows traditional disciplinary boundaries, and adopts the forms of combining classroom teaching with students' self-learning and teachers' instruction with topic discussion of students guided by teachers. In the teaching of the module course, discussion was adopted among students which involves teachers giving out the range of topics to students so that they can prepare for the topic of their intertest. After group discussion during class, the head of the teaching group gave comment on students' discussion and prize for whom had the best performance. Then, students were asked to summarize and analyze the questions raised during discussion after class. In conclusion, discussion class has stimulated students' interest in learning, improved their ability to think independently and encouraged them to demonstrate their abilities, which has achieved good teaching outcomes.
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Objective To propose a method for equipment parameter demonstration to improve whole-life cycle management of equipment.Methods The measures and principles of equipment parameter demonstration were put forward based on its present situation and problems.The significance of equipment parameter demonstration for whole-life management was discussed from the aspects of biding,bidding evaluation and post-procurement management.Results The method enhanced equipment procurement,saved human resource,materials and economic cost,and solved the problems such as single source,unutilized equipment and high maintenance cost.Conclusion The method of equipment parameter demonstration maximizes the benefits,and promotes whole-life management of medical equipment.
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Objective To complete an information chain to support stomatological materials purchase and providing manage-ment.MethodsThe main problems of the materials management information chain were analyzed.The system construction,technical architecture and function design were designed based on B/S and C/S structure.Results The vendor cloud platform completed the information chain for purchase and distribution of stomatological materials,and provided technical support to hospital consumables management.Conclusion The system normalizes stomatological materials management,guarantees the safety,timeliness and accuracy of materials supply,and thus is worthy applying practically.
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The present study aims to investigate the effect of Cbl-b, a member of E3 ubiquitin ligase family, on interleukin-1 (IL-1) pathway in synoviocytes. The protein expression levels of Cbl-b and IL-1-induced matrix metalloproteinase 13 (MMP-13) in synoviocytes were analyzed by Western blot. Collagen substrates were incubated with the conditioned medium collected from synoviocytes cultures and then subjected to SDS-PAGE for analysis of collagen degradation. The results showed that compared with wild-type cells, Cbl-b-deficient cells expressed more MMP-13 protein and had enhanced ability to degrade collagens under IL-1 stimulation. These data suggest that Cbl-b may negatively regulate IL-1-triggered degradation of collagen matrix in synoviocytes.
Subject(s)
Humans , Collagen , Metabolism , Epithelial Cells , Interleukin-1 , Metabolism , Matrix Metalloproteinase 13 , Metabolism , Proto-Oncogene Proteins c-cbl , Metabolism , Signal TransductionABSTRACT
Aiming at the disadvantages of traditional medical high value consumable management mode, bar code management is the only way which must be passed to the high value consumables in hospital management under the new situation. The bar code management system development in theMilitary Medical Projectmaterials management platform, scientific optimization of high value consumables management process, fully embodies the characteristics and advantages of bar code management. Not only to achieve a one relation between the high value consumables and patients, can also be integrated management supplies out of storage, charging and the financial sector, to achieve high value. The bar code in the ID, is the only identity code high value consumables, which maintained the patient's vital interests, and ensure that the hospital infection control requirements, the high value consumables in hospital benigndynamicmanagement system.
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Shigella flexneri is an infectious pathogen that causes dysentery to human, which remains a serious threat to public health, particularly in developing countries. In this study, the global protein expression patterns of S. flexneri during transition from exponential growth to stationary phase in vitro were analyzed by using 2-D PAGE combined with MALDI-TOF MS. In a time-course experiment with five time points, the relative abundance of 49 protein spots varied significantly. Interestingly, a putative outer membrane protein YciD (OmpW) was almost not detected in the exponential growth phase but became one of the most abundant proteins in the whole stationary-phase proteome. Some proteins regulated by the global regulator FNR were also significantly induced (such as AnsB, AspA, FrdAB, and KatG) or repressed (such as AceEF, OmpX, SodA, and SucAB) during the growth phase transition. These proteins may be the key effectors of the bacterial cell cycle or play important roles in the cellular maintenance and stress responses. Our expression profile data provide valuable information for the study of bacterial physiology and form the basis for future proteomic analyses of this pathogen.