ABSTRACT
The present study carried out a phytochemical investigation of the methanol extract of the branches and leaves of Clausena lansium and afforded nine carbazole alkaloids (compounds 1-9) including two new carbazole alkaloids, claulansiums A and B (compounds 1 and 2). The new compounds were elucidated on the basis of extensive spectroscopic data (MS, NMR, IR, and UV) and the known compounds were identified by comparing spectroscopic data with those reported in literature. All the isolated compounds were tested for their cytotoxic activity against A549 and Hela cancer cell lines. Our results showed that compounds 2-6 exhibited varying degrees of cytotoxicity to cancer cells, with IC values ranging from 8.67 to 98.89 μmol·L.
Subject(s)
Humans , A549 Cells , Alkaloids , Chemistry , Toxicity , Antineoplastic Agents , Chemistry , Toxicity , Carbazoles , Chemistry , Toxicity , Cell Line, Tumor , Cell Survival , Clausena , Chemistry , HeLa Cells , Molecular Structure , Plant Extracts , Chemistry , Toxicity , Plant Leaves , Chemistry , Plant Stems , Chemistry , Plants, Medicinal , ChemistryABSTRACT
The present study carried out a phytochemical investigation of the methanol extract of the branches and leaves of Clausena lansium and afforded nine carbazole alkaloids (compounds 1-9) including two new carbazole alkaloids, claulansiums A and B (compounds 1 and 2). The new compounds were elucidated on the basis of extensive spectroscopic data (MS, NMR, IR, and UV) and the known compounds were identified by comparing spectroscopic data with those reported in literature. All the isolated compounds were tested for their cytotoxic activity against A549 and Hela cancer cell lines. Our results showed that compounds 2-6 exhibited varying degrees of cytotoxicity to cancer cells, with IC values ranging from 8.67 to 98.89 μmol·L.
Subject(s)
Humans , A549 Cells , Alkaloids , Chemistry , Toxicity , Antineoplastic Agents , Chemistry , Toxicity , Carbazoles , Chemistry , Toxicity , Cell Line, Tumor , Cell Survival , Clausena , Chemistry , HeLa Cells , Molecular Structure , Plant Extracts , Chemistry , Toxicity , Plant Leaves , Chemistry , Plant Stems , Chemistry , Plants, Medicinal , ChemistryABSTRACT
This study is to investigate the effect of diet and gene on blood-fat trait of the individual mouse. One hundred and twenty mice were fed with high fat emulsion for 4 weeks. Then the genotypes of Glut-4 were analyzed by PCR-SSCP to investigate the effect on blood-fat traits and some organ performance of mice. After fed with high fat emulsion for 4 weeks, 98 mice suffered from hyperlipidemia, while 22 mice did not. And a T/A mutation was found in 188 targeted fragments we amplified at 8,521 site, and association analysis showed that the mice carried BB genotype had higher TC, TG and LW/BW level than those of the mice with AA genotype (P < 0.05). Different Glut-4 genotypes show different sensitivities to high fat emulsion on mice.