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Objective To investigate the efficacy of developable iodized oil-fluorouracil polylactic acid microspheres for treatment of New Zealand rabbit liver cancer model. Methods The model rabbits were randomly divided into three groups. The femoral arteries of the right hind limbswere separated, a microcatheter was inserted through the femoral artery to the hepatic artery via a catheter, and the corresponding drugs were injected to the peripheral arteries around the hepatic tumor tissue. The blank control group was not given any medicationi the drug control group was given fluorouracil injection and developable iodized oil injectioni and the experimental group was given self-made iodized oil-fluorouracil polylactic acid microspheres. Regular imaging was used to observe tumor growth and drug embolism status. Results Developable iodized oil-fluorouracil polylactic acid microspheres effectively achieved embolization around the tumor tissues, resulting in notable tumor cell apoptosis, and the effects were developable and durable. Conclusion Developable iodized oil-fluorouracil polylactic acid microspheres have sustained-release property and can be better developed, showing a promising clinical application future.
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Objective To prepare glycolic acid ethyl cellulose microspheres (GAECM) for hepatic artery embolization treatment and to investigate their in vitro release property. Methods GAECM was prepared using ethyl cellulose as a carrier, adding glycolic acid, by a double emulsion method. The appearance, particle size, drug loading, and encapsulation efficiency were used as indices to assess the roundness, smoothness, size uniformity, and drug contents of the microspheres. The in vitro release characteristics of the microspheres were determined by constant temperature oscillation dialysis. Results and conclusion The prepared GAECM has a smooth round appearance, with a mean diameter of (233.38±1. 62) μm, a mean drug loading of 114.32 μg/mg, an encapsulation efficiency of 78. 61%, and a release period of 72 h. The microspheres can meet the requirements of further animal experiments, which provides important reference for using chemical corrosion in drugs used for hepatic arterial embolization studies.
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Objective: To prepare visualized iodized oil-5-fluorouracil loaded polylactic acid(PLA) micropheres for hepatic artery embolism treatment. Methods: Biocompatible and biodegradable material PLA was used as vector and iodized oil was used as positive contrast agent to prepare 5-fluorouracil loaded microspheres using double emulsion method. The preparation technology of the microspheres was developed through optimization of appearance, size distribution, drug loading, and encapsulation efficiency by orthogonal-designing method. Results: The prepared PLA microspheres were round in shape and had a homogenous diameter distribution. Scanning electron microscope (SEM) showed a pored surface, with an average diameter of 100 μm. The encapsulation efficiency and drug content of microspheres were (63.34%±0.54%) and (10.78%±0.14%), respectively. Conclusion: We have successfully prepared the visualized iodized oil-5-fluorouracil PLA microspheres, which can release 5-fluorouracil in a controlled manner.
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<p><b>OBJECTIVE</b>To investigate the epidemiological characteristics of pathogens and their antimicrobial susceptibility in neonates with lower respiratory tract infection (LRTI).</p><p><b>METHODS</b>Sputum specimens for bacterial cultures were collected from 1173 neonates with LRTL between January 2005 and December 2006. Antibiotic susceptibility tests were performed after bacteria had been identified.</p><p><b>RESULTS</b>A total of 707 pathogenic strains (60.3%) were identified, including 521 (73.7%) Gram-negative bacilli, 106 (15.0%) Gram-positive bacilli, and 80 (11.3%) fungi. E Coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and enteric bacilli were common cultured Gram-negative bacilli. Most strains of Gram-negative bacilli were susceptible to meropenem, piperacillin/tazobactam, the fourth generation cephalosporin, cebfoperazone/sulbactam and amikacin. Staphylococcus aureus and coagula-negative staphylococci (CNS) were common in the cultured Gram-positive bacilli. Staphylococcus aureus and CNS were susceptible to vancomycin, ciprofloxacin and piperacillin/tazobactam but were resistant to Penicillin.</p><p><b>CONCLUSIONS</b>Gram-negative bacilli predominate the pathogens of LRTI in neonates. E Coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa are major pathogens.</p>